LOCUS       sequence-424318-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human PHGDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI C-FLAG PHGDH T57A
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4338..4361
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4430..4659
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4642..4661
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4836..5424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4889..4909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5307..5318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5831..6011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6037..6054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6043..6267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6313..6741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6400..6419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6610..6631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6750..6770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6755..7084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6935..7070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6997..7016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7132..7179
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7198..7572
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7702..7823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7739..7758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7793..7812
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7872..7888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7872..7888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7885..7907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7896..7912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7920..7926,7927..7944,7945..7950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7965..7986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8103..8120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8274..8862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8354..8373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9033..9824,9825..9893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9656..9675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9894..9998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10073..10092)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10264..10456,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaact
     2701 ctagagaatt cggatccatg gcttttgcaa atctgcggaa agtgctcatc agtgacagcc
     2761 tggacccttg ctgccggaag atcttgcaag atggagggct gcaggtggtg gaaaagcaga
     2821 accttagcaa agaggagctg atagcggagc tgcaggactg tgaaggcctt attgttcgct
     2881 ctgccgccaa ggtgaccgct gatgtcatca acgcagctga gaaactccag gtggtgggca
     2941 gggctggcac aggtgtggac aatgtggatc tggaggccgc aacaaggaag ggcatcttgg
     3001 ttatgaacac ccccaatggg aacagcctca gtgccgcaga actcacttgt ggaatgatca
     3061 tgtgcctggc caggcagatt ccccaggcga cggcttcgat gaaggacggc aaatgggagc
     3121 ggaagaagtt catgggaaca gagctgaatg gaaagaccct gggaattctt ggcctgggca
     3181 ggattgggag agaggtagct acccggatgc agtcctttgg gatgaagact atagggtatg
     3241 accccatcat ttccccagag gtctcggcct cctttggtgt tcagcagctg cccctggagg
     3301 agatctggcc tctctgtgat ttcatcactg tgcacactcc tctcctgccc tccacgacag
     3361 gcttgctgaa tgacaacacc tttgcccagt gcaagaaggg ggtgcgtgtg gtgaactgtg
     3421 cccgtggagg gatcgtggac gaaggcgccc tgctccgggc cctgcagtct ggccagtgtg
     3481 ccggggctgc actggacgtg tttacggaag agccgccacg ggaccgggcc ttggtggacc
     3541 atgagaatgt catcagctgt ccccacctgg gtgccagcac caaggaggct cagagccgct
     3601 gtggggagga aattgctgtt cagttcgtgg acatggtgaa ggggaaatct ctcacggggg
     3661 ttgtgaatgc ccaggccctt accagtgcct tctctccaca caccaagcct tggattggtc
     3721 tggcagaagc tctggggaca ctgatgcgag cctgggctgg gtcccccaaa gggaccatcc
     3781 aggtgataac acagggaaca tccctgaaga atgctgggaa ctgcctaagc cccgcagtca
     3841 ttgtcggcct cctgaaagag gcttccaagc aggcggatgt gaacttggtg aacgctaagc
     3901 tgctggtgaa agaggctggc ctcaatgtca ccacctccca cagccctgct gcaccagggg
     3961 agcaaggctt cggggaatgc ctcctggccg tggccctggc aggcgcccct taccaggctg
     4021 tgggcttggt ccaaggcact acacctgtac tgcaggggct caatggagct gtcttcaggc
     4081 cagaagtgcc tctccgcagg gacctgcccc tgctcctatt ccggactcag acctctgacc
     4141 ctgcaatgct gcctaccatg attggcctcc tggcagaggc aggcgtgcgg ctgctgtcct
     4201 accagacttc actggtgtca gatggggaga cctggcacgt catgggcatc tcctccttgc
     4261 tgcccagcct ggaagcgtgg aagcagcatg tgactgaagc cttccagttc cacttcctcg
     4321 agccatggaa gcttatggac tacaaagacg atgacgacaa ggatatctaa ctgactgaac
     4381 cggtggtacc gaggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc
     4441 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat
     4501 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa
     4561 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact
     4621 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac
     4681 tgcaggtcga gcatgcatct agggcggcca attccgccga tcctggaaaa acatggagca
     4741 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     4801 gaggaggagg tgggttttcc agtcacacct cagacaatca acctctggat tacaaaattt
     4861 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4921 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4981 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5041 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5101 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5161 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5221 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     5281 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5341 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5401 tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac
     5461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5521 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5581 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     5641 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     5701 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5761 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     5821 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6001 aatctctagc agggccgctt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6061 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6121 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6181 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6241 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6301 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6721 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7021 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7081 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     7141 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     7201 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     7261 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     7321 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     7381 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     7441 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     7501 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     7561 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7621 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7681 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7801 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10021 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    10081 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    10141 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    10201 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
    10261 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
    10321 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    10381 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
    10441 ggactttcca ttgacg
//
