LOCUS       sequence-424348-       12594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted knockdown in neurons. 
            CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 
            promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Liau WS, Zhao Q, Bademosi A, Gormal RS, Gong H, Marshall PR, 
            Periyakaruppiah A, Madugalle SU, Zajaczkowski EL, Leighton LJ, Ren 
            H, Musgrove M, Davies J, Rauch S, He C, Dickinson BC, Li X, Wei W, 
            Meunier FA, Fernandez-Moya SM, Kiebler MA, Srinivasan B, Banerjee S,
            Clark M, Spitale RC, Bredy TW
  TITLE     Fear extinction is regulated by the activity of long noncoding RNAs 
            at the synapse.
  JOURNAL   Nat Commun. 2023 Nov 22;14(1):7616. doi: 10.1038/s41467-023-43535-1.
  PUBMED    37993455
REFERENCE   2  (bases 1 to 12594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-11-22"; volume: "14(1):7616. doi"; pages: " 
            10.1038/s41467-023-43535-1"
COMMENT     Sequence Label: CIRTS-Gas5
FEATURES             Location/Qualifiers
     source          1..12594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3777..3798)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4038..4057)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4385..4406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4458..4511
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5028..5054
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5658..5681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6715..6963
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6715..6735
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6886..6905
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7286..7874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7339..7359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7757..7768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7877..7893)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7878..7894)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8399..8579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8605..8622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8611..8835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8881..9309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8968..8987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9178..9199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9318..9338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9323..9652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9503..9638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9565..9584
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9700..9747
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9766..10140
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10270..10391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10307..10326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10361..10380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10440..10456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10440..10456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10453..10475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10464..10480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10488..10494,10495..10512,10513..10518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10533..10554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10671..10688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10842..11430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10922..10941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11601..12392,12393..12461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12224..12243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12462..12566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3901 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4201 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4441 tgtacaaggg ctccggcgag ggcaggggaa gtctactaac atgcggggac gtggaggaaa
     4501 atcccggccc aaccggtatg ggaattatca acacccttca aaaatactac tgtcgcgtcc
     4561 gtgggggtcg ttgcgcagtc ctgtcgtgcc tgcctaaaga ggagcagatc ggtaaatgct
     4621 caacccgtgg acgtaagtgc tgtcgtcgca agaaaggcgg ttctggaggc tcagggggga
     4681 gcggagggtc cggtggatca ggaggctcca tggcagttcc cgagacccgc cctaaccaca
     4741 ctatttatat caacaacctc aatagcaaga tcaagaagga tgagctaaaa aagtccctgt
     4801 acgccatctt ctcccagttt ggccagatcc tggatatcct ggtaccgcgg cagcgtaccc
     4861 cgaggggcca ggcctttgtc atcttcaagg aggtcagcag cgccaccaac gccctgcgct
     4921 ccatgcaggg tttccctttc tatgacaaac ctatgcgtat ccagtatgcc aagaccgaca
     4981 aacgtatccc ggccaagatg aaaggcacct tcgtgggatc ccttcaactg cctccacttg
     5041 aaagactgac actgggcggt agtggaggaa gcggggggag cggagggagt ggtggatccg
     5101 gcggctccat ggaattggag atccgtccgc ttttcctggt tcccgatacc aatgggttca
     5161 ttgaccactt agcgagctta gcccgtctgc ttgagagccg taagtacatt ctggtggttc
     5221 ctcttattgt catcaacgag ttagacggcc ttgcgaaagg ccaggaaaca gaccatcgtg
     5281 cgggaggcta tgcgcgtgtc gtgcaagaaa aggctcgcaa gagcattgag tttttggagc
     5341 aacgtttcga gtcgcgtgac tcctgtttac gtgcacttac cagccgtgga aatgaacttg
     5401 agtcaatcgc atttcgctct gaagatatca ctgggcagtt aggtaacaat gacgatttga
     5461 ttttgtcatg ctgcctgcac tattgtaaag acaaggcaaa ggactttatg cccgcgtcga
     5521 aggaagagcc gatccgtttg ttgcgtgagg tggtattgct tactgatgat cgcaacctgc
     5581 gcgttaaggc tcttacacgc aacgtccctg tgcgtgacat cccagctttc ttaacatggg
     5641 cccaggtcgg gggatccgat tacaaggacg acgatgacaa gtaggctagc gggagcctct
     5701 gccctcctcc tgcagcccgg atggctctcc tctgtttttg gtttgcttcc ttttgtttgc
     5761 ccttcttttg ggtgaccggg gtggccacca ttcctgtccc tgcccagtgg ggaggggagc
     5821 aaggccttct gccaagagga ggagcatgtc ccctgccact gttgcatgca ccccggccct
     5881 gtgactctgt gtgcaagccc agcagcctgt ggggcagggt gccaagaggg gacgggacgg
     5941 acgttccagg acagagggag tcgaggatgt gatccaggac gggtgggggg tggaagggtg
     6001 ggaaagggca ggaaggcctt ggaagctaac atgctttcac tactggaggg ggacggatag
     6061 agcggatggc actcgggtct cactgacgct gttctgaaac catctggctg gctttctgag
     6121 gtcaggctgg gtagggggct gtcatcggcc atactgctga tcacacttgc cctcatccgt
     6181 ccagcgatct cacgctgttc tgtaaatacc tggtgctaac atcccatgcc gctcccccat
     6241 ggcaccccca gcctcctgcc cagttcaggc tggtccagga atggatgtgg gggatggtcg
     6301 ctgtgtaacg tgctggtgat ctttttcccc tacccctttt atgaccctta aaactttgat
     6361 tttgtcctaa acatgccggg ccaactaaag ggtggggaga ggagagacga gccccaccat
     6421 gctcacaaga accaacctgc aataaacact tctgtgggcc gccgcgcaga gcacgcagcg
     6481 ggcacctcct gcccctccat ggcgacttgg catcgctttc tgcccgcctc tgccctcgcc
     6541 ctgcctgccc caccccccag cggagagcat gatccgcgcc cttgcttctg actctagcct
     6601 ctgggacaca tcagtcaacg tgggcagttc agtctgggtt cgttcctttc tctgttctca
     6661 tctggccccc cagggcgatc ttgttgcttt ttgctgggac ctgcccagtc tagagagggc
     6721 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     6781 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     6841 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     6901 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     6961 accggccaga tctgagcctg ggagctctct ggccttatta gcaagccagc caaatgaaca
     7021 agcatgcaat tttttagcaa gccagccaaa tgaacaagca tgcaattttt tagcaagcca
     7081 gccaaatgaa caagcatgca attttttagc aagccagcca aatgaacaag catgcaattt
     7141 tttagcaagc cagccaaatg aacaagcatg caatttttta gcaagccagc caaatgaaca
     7201 agcatgcaat tttttagcaa gccagccaaa tgaacaagca tgcaattttt tgagacgtct
     7261 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     7321 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7381 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7441 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7501 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7561 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7621 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7681 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7741 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7801 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7861 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     7921 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7981 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     8041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8101 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     8161 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     8221 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     8281 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     8341 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     8401 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8461 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8521 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8581 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8761 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8821 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8881 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8941 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     9001 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9061 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     9121 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9181 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     9241 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9301 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     9601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9661 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     9721 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     9781 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     9841 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     9901 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9961 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
    10021 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
    10081 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
    10141 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10201 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10261 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10381 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    10441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10501 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    10681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    11461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    11521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    12181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
