LOCUS       sequence-424366-       11235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of hMyo19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Jiao H, Jiang D, Hu X, Du W, Ji L, Yang Y, Li X, Sho T, Wang X, Li 
            Y, Wu YT, Wei YH, Hu X, Yu L
  TITLE     Mitocytosis, a migrasome-mediated mitochondrial quality-control 
            process.
  JOURNAL   Cell. 2021 May 27;184(11):2896-2910.e13. doi: 
            10.1016/j.cell.2021.04.027.
  PUBMED    34048705
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04"; journalName: "Cell"; date: "2021-05-27- 
            27"; volume: "184"; issue: "11"; pages: "2896-2910.e13"
COMMENT     Sequence Label: pB-Neo-GFP-hMyo19 iso2
FEATURES             Location/Qualifiers
     source          1..11235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          658..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1779..1781,1782..1784,1785..2495)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1779..1781,1782..1784,1785..2495)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1824..1845)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2085..2104)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2432..2453
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5430..5495
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5520..5540
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5544..5588
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(5597..5614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5603..5827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5912..6029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6075..6108
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6279..6341
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6365..6383)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6404..6420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6428..6444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6452..6458,6459..6476,6477..6482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6497..6518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6635..6652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6806..7394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6886..6905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7565..8356,8357..8425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8188..8207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8426..8530)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8556..9011)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8693..8714)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8905..8924
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9138..9160
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9152..9169
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9153..9169
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9176..9195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9176..9194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9213..9247
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9528..9548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9533..9862
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9713..9848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9775..9794
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9929..10723
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9983..10002)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10593..10612
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10899..11020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10936..10955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10990..11009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11069..11085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11093..11109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11117..11123,11124..11141,11142..11147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11162..11183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    11187..11220
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgccr ggsggggsgg gggggggggg gggggggggs
      541 ggggsgrggs ggrgrggkgs ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggkggg ggtgccgggc ggggcggggc
     1261 cgccycgggc cggggggggc kcgggggggg ggcgcggcgg cccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcctc gacggtatcg
     1741 ataagctatc acaagtttgt acagccacca tgcccgggat ggtgagcaag ggcgaggagc
     1801 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1861 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1921 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1981 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2041 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2101 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2161 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2221 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2281 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2341 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2401 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2461 ccgggatcac tctcggcatg gacgagctat acaagggatc cacgcgtatg ctccagcagg
     2521 tcaatggcca caatccgggg tctgatggcc aagccaggga gtacctcaga gaagacctgc
     2581 aggagttcct gggtggggag gtcctgctgt acaaactgga tgacctcacc agggtgaatc
     2641 ctgtgacact agagacagtc ctgaggtgcc tgcaggcccg gtacatggca gacacattct
     2701 acaccaatgc tggctgcacc ctggtagcct tgaacccctt caagcctgtt cctcagctct
     2761 actcgcccga gctaatgaga gagtaccatg ctgcgcctca gccccagaaa ctgaagcccc
     2821 atgtgttcac tgtgggtgaa cagacctaca ggaatgtcaa gagcctgatt gaaccagtca
     2881 accagtctat tgttgtcagt ggagagagtg gtgctggaaa gacatggacg tctcgctgcc
     2941 tgatgaagtt ctatgctgtg gtggccacct cacctgcatc ttgggagagc cacaagattg
     3001 cagagaggat agaacagagg atcctgaact ccaaccctgt catggaagct tttgggaatg
     3061 cgtgtacact gaggaataac aacagcagtc gctttgggaa gttcatccag ctccagctga
     3121 acagggctca gcaaatgact ggagccgcag tccagaccta cctcctagag aaaactcgag
     3181 tggcctgtca ggcttccagt gagaggaact tccacatctt ctatcagatt tgcaaaggag
     3241 ccagtgagga cgagaggctc cagtggcacc ttcctgaggg agctgccttc tcctggctgc
     3301 ccaacccaga gaggagctta gaagaggatt gttttgaggt gaccagagag gccatgctcc
     3361 atttgggcat tgacacccct acccagaaca acatctttaa ggtcctagct ggactgctgc
     3421 accttggcaa tatccagttt gctgcctccg aggatgaagc ccagccctgc cagccgatgg
     3481 atgatgccaa gtactctgtc aggacggcag cctcgctgct ggggctccca gaggacgtgc
     3541 tgctggagat ggtgcagatt agaaccatca gggcaggcag acagcagcag gtgttccgga
     3601 agccctgcgc ccgagccgag tgtgacaccc gtagagactg cctggccaaa ctgatctatg
     3661 cgcggttgtt tgactggctg gtatcagtga tcaacagcag catctgtgca gacaccgact
     3721 cgtggaccac tttcataggc ctgctggatg tgtatggatt tgaatcattt cctgacaaca
     3781 gtctggaaca gttgtgcatc aactacgcca atgagaagct gcagcagcat tttgtggctc
     3841 actacctaag ggcccagcag gaggaatacg cagttgaggg cctggagtgg tcattcatca
     3901 actaccagga caaccagccc tgtttggatc tcattgaggg aagccccatc agcatctgct
     3961 ccctcataaa tgaggaatgc cgcctcaatc gacccagcag cgcagcccag ctccagacac
     4021 gcattgagac tgccctggca ggcagcccct gcctgggcca caataagctc agccgggagc
     4081 ccagcttcat tgtggtgcat tatgcggggc ctgtgcggta ccacacagca ggcctggtgg
     4141 agaagaacaa ggaccctatc ccacctgagc tgaccaggct cctgcagcaa tcccaggacc
     4201 ccctgctcat ggggctgttt cctactaacc ccaaagagaa gacccaggag gaaccccctg
     4261 gccagagcag ggcccctgtg ttgaccgtgg tgtccaagtt caaggcctca ctggagcagc
     4321 ttctgcaggt cctacacagc accacgcccc actacattcg ctgcatcaag cccaacagcc
     4381 agggccaggc gcagaccttt ctccaagagg aggtcctgag ccagctggag gcctgtggcc
     4441 tcgtggagac catccatatc agtgctgctg gcttccccat ccgggtctct caccgaaact
     4501 ttgtagaacg atacaagtta ctaagaaggc ttcatccttg cacatcctct ggccccgaca
     4561 gcccatatcc tgccaaaggg ctccctgaat ggtgtccaca cagcgaggaa gccacgcttg
     4621 aacctctcat ccaggacatt ctccacactc tgccggtcct aactcaggca gcagccataa
     4681 ctggtgactc ggctgaggcc atgccagccc ccatgcactg tggcaggacc aaggtgttca
     4741 tgactgactc tatgctggag cttctggaat gtgggcgtgc ccgggtgctg gagcagtgtg
     4801 cccgctgcat ccagggtggc tggaggcgac accggcaccg agagcaggag cggcagtggc
     4861 gggccgtcat gctcatccag gcagccattc gttcctggtt aactcggaaa cacatccaga
     4921 ggctgcatgc agctgccaca gtcatcaagc gtgcatggca gaagtggaga atcagaatgg
     4981 cctgccttgc tgctaaagag ctggatggtg tggaagaaaa acacttctct caagctccct
     5041 gttccctgag cacctcgccg ctgcagacca ggctcctgga ggcaataatc cgcctctggc
     5101 ccctgggact ggtcctggcc aatacggcta tgggtgtagg cagctttcag aggaaattag
     5161 tggtctgggc ttgcctccag ctccccaggg gcagccccag tagctacact gtccagacag
     5221 cacaagacca ggctggtgtc acgtccatcc gagcgctgcc tcagggatcg ataaagtttc
     5281 actgcagaaa gtctccactg cggtatgctg acatctgccc tgaaccttca ccctacagca
     5341 ttacaggctt taatcagatt ctgctggaaa gacacaggct gatccacgtg acctcttctg
     5401 ccttcactgg gctggggtga gctagcatgg actacaaaga ccatgacggt gattataaag
     5461 atcatgatat cgattacaag gatgacgatg acaagggatc tgcaagaaga gcatctgtgg
     5521 aaaacctgta ttttcagggg tccggcctga acgacatctt cgaggctcag aaaatcgaat
     5581 ggcacgaata atctagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5641 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5701 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5761 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5821 tctatggctt ctgaggcggt taattaaaat aaaactagag cgggcccggt actaaccaaa
     5881 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     5941 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     6001 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga
     6061 gcggccgcgt cgagataact tcgtataatg tatgctatac gaagttatgt tttgttactt
     6121 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     6181 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     6241 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     6301 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaagagc tccagctttt
     6361 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     6421 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6481 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6541 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6661 tcgttcggct gcggcgagag gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8521 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     8581 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8641 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8701 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8761 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8821 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8881 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8941 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9001 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     9181 cgactcacta tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg
     9241 acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc
     9301 atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa
     9361 gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt
     9421 acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac
     9481 gtgataactt attatatata tattttcttg ttatagatta attctgtgga atgtgtgtca
     9541 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9781 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9841 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9901 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     9961 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
    10021 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    10081 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
    10141 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    10201 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    10261 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    10321 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    10381 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    10441 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
    10501 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    10561 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    10621 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    10681 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
    10741 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    10801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    10921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    11041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    11101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11161 aatgagtgag ctaactcaca ttaatcataa cttcgtataa tgtatgctat acgaagttat
    11221 gcggccctcg aggtc
//
