LOCUS       sequence-424436-        2247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2200..2223
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccagttt cgcaatcctg ctccaaatct atgaggtcca acgcggccct
     1261 gaggaagagt ctcttctgcg cactttgtct tttgtggggt gcggcgtcag tttctgtgcg
     1321 ctgaccacta cctttctcct gttccttgtg gccggagtac ctaagtctga acgcacgacc
     1381 gtgcacaaga acctgacctt ttccttggct tctgccgagg gctttctcat gacgtccgaa
     1441 tgggccaaag caaacgaggt tgcctgtgtg gcagtgactg tggcgatgca tttcctgttc
     1501 ctggtggcgt tcagttggat gcttgtggaa ggcctgcttt tgtggcggaa ggtagtcgcc
     1561 gtgagcatgc accccggacc tggaatgcgt ctgtaccacg ctaccggctg gggagtgcca
     1621 gttggcattg tggccgtgac gctggctatg ctgccccatg actacgtcgc tccgggccac
     1681 tgctggctga atgtgcatac caatgccatt tgggctttcg tcggacccgt gctgttcgtc
     1741 ctgaccgcta acacctgtat tctggcccgt gttgtcatga tcacggtgag ctcagcccgc
     1801 cggcgcgccc gcatgttgtc tccccagccg tgtctccagc aacagatctg gacccagatc
     1861 tgggcgaccg tcaagcccgt gctggtgctg ctccccgtgt tgggtctcac ctggctggca
     1921 ggcatcctgg tgcacctgtc ccccgcctgg gcgtacgcgg ctgttggcct gaactccatc
     1981 caggggctgt acatttttct ggtgtacgca gcttgcaatg aggaagtgcg ctccgctctg
     2041 cagcgtatgg cagaaaagaa agtcgcagaa gtgttgagag ccctgggcgt gtggggcggt
     2101 gcggccaagg agcacagcct gcccttcagt gttctgcctc tgttcctgcc tcccaagcct
     2161 agcacccctc gccatccgct caaggctccc gccggttctg actacaaaga cgatgacgac
     2221 aagtgaagat ctgacaaaac tcacaca
//
