LOCUS       sequence-424442-        2298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRF1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2251..2274
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaacctcctt ctccatattg atgtcacctt ttgtcccctc tacaatcttc
     1261 cccgttgtaa aatggatcac ctatgtggga ctgggtatct ccattggaag tctcatttta
     1321 tgcctgatca tcgaggcttt gttttggaag cagattaaaa aaagccaaac ctctcacaca
     1381 cgtcgtattt gcatggtgaa catagccctg tccctcttga ttgctgatgt ctggtttatt
     1441 gttggtgcca cagtggacac cacggtgaac ccttctggag tctgcacagc tgctgtgttc
     1501 tttacacact tcttctacct ctctttgttc ttctggatgc tcatgcttgg catcctgctg
     1561 gcttaccgga tcatcctcgt gttccatcac atggcccagc atttgatgat ggctgttgga
     1621 ttttgcctgg gttatgggtg ccctctcatt atatctgtca ttaccattgc tgtcacacaa
     1681 cctagcaata cctacaaaag gaaagatgtg tgttggctta actggtccaa tggaagcaaa
     1741 ccactcctgg cttttgttgt ccctgcactg gctattgtgg ctgtgaactt cgttgtggtg
     1801 ctgctagttc tcacaaagct ctggaggccg actgttgggg aaagactgag tcgggatgac
     1861 aaggccacca tcatccgcgt ggggaagagc ctcctcattc tgacccctct gctagggctc
     1921 acctggggct ttggaatagg aacaatagtg gacagccaga atctggcttg gcatgttatt
     1981 tttgctttac tcaatgcatt ccagggattt tttatcttat gctttggaat actcttggac
     2041 agtaagctgc gacaacttct gttcaacaag ttgtctgcct taagttcttg gaagcaaaca
     2101 gaaaagcaaa actcatcaga tttatctgcc aaacccaaat tctcaaagcc tttcaaccca
     2161 ctgcaaaaca aaggccatta tgcattttct catactggag attcctccga caacatcatg
     2221 ctaactcagt ttgtctcaaa tgaaggttct gactacaaag acgatgacga caagtgaaga
     2281 tctgacaaaa ctcacaca
//
