LOCUS       sequence-424454-        3174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..3174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3127..3150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccaactt tgccgtgctc atggcccacc gcgaaatcta ccagggccgc
     1261 attaacgagc tcctgcttag tgtgatcaca tgggttggca ttgtgatcag cctggtgtgc
     1321 ctggccattt gtatctccac tttctgtttt ctcagaggac tgcagaccga caggaacacc
     1381 attcacaaga acctctgcat taacctgttc ctcgctgagc tcctgttcct ggttgggatc
     1441 gacaagaccc agtacgaaat cgcttgcccc atcttcgctg gtctcctgca ttattttttc
     1501 ctggctgcct tttcctggct gtgcctggag ggtgtccatc tctacttgct gttggtagag
     1561 gtctttgaga gcgagtattc ccgcaccaag tactattacc tgggtggcta ctgtttccca
     1621 gctctggttg tgggcatcgc cgcagccatc gattacagat cctacgggac agagaaggct
     1681 tgttggctcc gcgtcgataa ctatttcatt tggtccttca tcggtccagt ttcttttgtc
     1741 atcgtcgtga acttggtatt cctgatggtg acactgcaca agatgatccg cagcagttcc
     1801 gtgttgaagc ccgattccag ccgtctggat aacatcaagt cctgggcgct gggcgccatc
     1861 gcgcttttgt ttcttctggg gttgacctgg gcctttggcc tcctgttcat caataaagaa
     1921 agcgtcgtta tggcttatct gttcactacc ttcaacgcct tccagggcgt gttcattttc
     1981 gttttccatt gcgcgctgca gaaaaaggtc cacaaggaat attccaagtg cctgcgccat
     2041 agctattgtt gcattcggag cccgccaggt ggcacacacg gatctctgaa gacatccgcg
     2101 atgcgttcta acacccggta ctataccggt acccagtccc gcattcggcg tatgtggaac
     2161 gacaccgtgc gcaaacagac cgagtcctct tttatggctg gcgacatcaa ctcaaccccg
     2221 accctcaacc ggggtaccat gggcaaccat ctgcttacca acccagtact gcagccccgt
     2281 ggcggaacaa gtccctacaa cacccttatt gcggagtctg tggggttcaa cccgagctca
     2341 ccgcctgtct tcaatagccc cggttcttat cgggagccga agcatcccct gggtggcaga
     2401 gaagcctgcg gcatggacac cctgccgctt aacggtaact tcaataactc ctattccctg
     2461 cggagtggcg actttccgcc tggcgatggt ggcccagaac ctccaagggg tcgtaacctg
     2521 gctgatgctg ccgcgttcga aaagatgatc attagcgagt tggttcacaa caatctgaga
     2581 ggtagctctt ccgccgcgaa aggaccgcct ccgcctgagc ctcccgttcc acctgtgcct
     2641 ggtggaggcg gggaggaaga ggccggcggt ccaggcgggg ccgacagggc tgagatcgaa
     2701 ctgctctaca aggcccttga agagcccctg ctcctgcctc gcgcgcagtc tgtgctgtat
     2761 cagtccgacc tggatgagag tgaatcctgc accgctgaag atggagctac ctcccgtcct
     2821 ctgtccagtc ctcccggacg cgattccctg tatgctagtg gcgcaaacct gagggatagt
     2881 cccagctatc cagactcctc tcccgaggga ccatctgagg cactcccacc tccaccgcct
     2941 gctccacctg gcccaccgga aatctactat acgagtcgtc cgccagcgct ggtcgctcgc
     3001 aatccactgc aaggttatta ccaggtacgc cgtcctagcc atgagggcta tcttgccgca
     3061 cccggcctgg aggggcctgg ccctgacggt gatggacaga tgcagttggt cacctcactg
     3121 ggttctgact acaaagacga tgacgacaag tgaagatctg acaaaactca caca
//
