LOCUS       sequence-424472-        2187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     CDS             2140..2163
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aagcgcttac ttctcaagaa gaggaccctg tgctgaccgt tatcacctac
     1261 gtaggactct cagtctccct cttgtgcctt ctcctggctg ccctcacttt cctcctgtgt
     1321 aaggcaattc gcaacacttc taccagcctg catctccagc tgtctctgtg cctgttcctc
     1381 gcccacctcc tgttcctggt tgggatcgat cgtactgaac ctaaggttct gtgttccatt
     1441 atcgccggtg ctctccatta tctttatctg gctgccttta cctggatgct tctggagggc
     1501 gtgcacctgt tcctgaccgc taggaacctg actgtggtta actacagctc tatcaacaga
     1561 ctcatgaagt ggattatgtt ccctgtgggc tatggggtgc ctgccgtgac tgtcgccatc
     1621 agtgctgcat catggccaca cctgtacggc acagccgacc ggtgctggct gcacctggac
     1681 cagggattta tgtggagctt tctggggccc gtttgcgcta ttttctctgc taacctggtg
     1741 ctgttcattt tggtcttctg gatcctcaaa cgtaagctta gctctctgaa ctctgaagtg
     1801 tccacgatcc agaatacccg catgctggcc ttcaaagcca cagcccagct gtttatcctg
     1861 ggctgtacct ggtgtctggg cttgctccag gtcggaccgg cagcccaggt gatggcctac
     1921 ctgtttacta ttatcaacag cttgcagggt tttttcatct ttcttgtgta ttgcttgctg
     1981 tcccaacagg tgcagaagca ataccagaag tggttccggg agatcgtaaa gtccaagagc
     2041 gagtccgaaa cctatacact gtctagcaag atgggccctg actctaaacc atccgaggga
     2101 gatgtttttc ccggccaggt aaaacgcaag tacggttctg actacaaaga cgatgacgac
     2161 aagtgaagat ctgacaaaac tcacaca
//
