LOCUS       sequence-424506-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombine floxed alleles in newborn neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Williams MR, DeSpenza T Jr, Li M, Gulledge AT, Luikart BW
  TITLE     Hyperactivity of newborn Pten knock-out neurons results from 
            increased excitatory synaptic drive.
  JOURNAL   J Neurosci. 2015 Jan 21;35(3):943-59. doi: 
            10.1523/JNEUROSCI.3144-14.2015.
  PUBMED    25609613
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3144-14"; journalName: "J Neurosci"; date: 
            "2015-01-21- 21"; volume: "35"; issue: "3"; pages: "943-59"
COMMENT     Sequence Label: pRubiC-T2A-cre
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(96..115)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(1234..1650)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1250..1266)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1282..1304)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    1851..2050
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2113..2288
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(2289..2492)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2318..2338)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2493..2872
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2972..2990)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3058..3162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3163..3231,3232..4023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3381..3400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4194..4782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4683..4702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4936..4953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5034..5053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(5075..5404)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5089..5224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5389..5409
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     5545..5567
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             5670..6095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     6210..6226
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6211..6227
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6230..6818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6336..6347
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6745..6765
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6842..7870)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKCFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(7877..7930)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7931..8638)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPDDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8206..8225)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8475..8493
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8635..8644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(8680..8728,1..1163))
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gtctaacact gaaaattaca tattgaccca aatgattaca tttcaaaagg tgcctaaaaa
       61 acttcacaaa acacactcgc caaccccgag cgcatagttc aaaaccggag cttcagctac
      121 ttaagaagat aggtacataa aaccgaccaa agaaactgac gcctcactta tccctcccct
      181 caccagaggt ccggcgcctg tcgattcagg agagcctacc ctaggcccga accctgcgtc
      241 ctgcgacgga gaaaagccta ccgcacacct accggcaggt ggccccaccc tgcattataa
      301 gccaacagaa cgggtgacgt cacgacacga cgagggcgcg cgctcccaaa ggtacgggtg
      361 cactgcccaa cggcaccgcc ataactgccg cccccgcaac agacgacaaa ccgagttctc
      421 cagtcagtga caaacttcac gtcagggtcc ccagatggtg ccccagccca tctcacccga
      481 ataagagctt tcccgcatta gcgaaggcct caagaccttg ggttcttgcc gcccaccatg
      541 ccccccacct tgtttcaacg acctcacagc ccgcctcaca agcgtcttcc attcaagact
      601 cgggaacagc cgccattttg ctgcgctccc cccaaccccc agttcagggc aaccttgctc
      661 gcggacccag actacagccc ttggcggtct ctccacacgc ttccgtccca ccgagcggcc
      721 cggcggccac gaaagccccg gccagcccag cagcccgcta ctcaccaagt gacgatcaca
      781 gcgatccaca aacaagaacc gcgacccaaa tcccggctgc gacggaacta gctgtgccac
      841 acccggcgcg tccttatata atcatcggcg ttcaccgccc cacggagatc cctccgcaga
      901 atcgccgaga agggactact tttcctcgcc tgttccgctc tctggaaaga aaaccagtgc
      961 cctagagtca cccaagtccc gtcctaaaat gtccttctgc tgatactggg gttctaaggc
     1021 cgagtcttat gagcagcggg ccgctgtcct gagcgtccgg gcggaaggat caggacgctc
     1081 gctgcgccct tcgtctgacg tggcagcgct cgccgtgagg aggggggcgc ccgcgggagg
     1141 cgccaaaacc cggcgcggag gccagatctg gagccgacac gggttaatta aggttcgaag
     1201 tgtgtgcggc cgctgtgtga gatcttcaat tccggcgcct agagaaggag tgagggctgg
     1261 ataaagggag gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga
     1321 tggaggaaga ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc
     1381 aaaagccaag gcttcccagg tcacgatgta ggggacctgg tctgggtgtc catgcgggcc
     1441 aggtgaaaag accttgatct taacctgggt gatgaggtct cggttaaagg tgccgtctcg
     1501 cggccatccg acgttaaagg ttggccattc tgcagagcag aaggtaaccc aacgtctctt
     1561 cttgacatct accgactggt tgtgagcgat ccgctcgaca tctttccagt gacctaaggt
     1621 caaacttaag ggagtggtaa cagtctggcc ctaattttca gacaaataca gaaacacagt
     1681 cagacagaga caacacagaa cgatgctgca gcgctgcagc agacaagacg cgcggcttcg
     1741 gttccaaacc gaaagcaaaa attcagacgg aggcgggaac tgttttaggt tctcgtctcc
     1801 taccagaacc acatatcctg acggggtcgg attccacatc gactcccttc ctcaggtcgg
     1861 gccacaaaaa cggcccccaa agtccctggg acgtctccca gggttgcggc cgggtgttca
     1921 gaactcgtca gttccaccac gggtccgcca gatacagagc tagttagcta actagtacag
     1981 acgcaggcgc ataacatcaa acatagacac tagacaatcg gacagacaca gataagttgc
     2041 tggccagctt acctcccggt ggtgggtcgg tggtccctgg gcaggggtct ccaaatcccg
     2101 gacgagcccc caaatgaaag acccccgtcg tgggtagtca atcactcaga ggagaccctc
     2161 ccaaggaaca gcgagaccac gattcggatg caaacagcaa gaggctttat tgggaatacg
     2221 ggtacccggg cgacgcagtc tatcggaaga ctggcgcgcc gagtgagggg ttgtgggctc
     2281 ttttattgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     2341 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     2401 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     2461 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     2521 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     2581 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     2641 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     2701 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     2761 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     2821 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc
     2881 ggtaatgttg gacatgagcc aatataaatg tacatattat gatatggata caacgtatgc
     2941 aatggccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3001 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3061 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     3421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     3721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4321 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     4981 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     5041 tgctctgatg ccgcatagtt aagccagtaa gctttttgca aaagcctagg cctccaaaaa
     5101 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     5161 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     5221 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     5281 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     5341 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     5401 acacattcca cagccggatc tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5461 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5521 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5581 caccgaaacg cgcgaggcag ccagcttacc tcccggtggt gggtcggtgg tccctgggca
     5641 ggggtctccc gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat
     5701 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     5761 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     5821 tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag
     5881 aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc
     5941 ctggaaacat ctgatggttc tctagggtcg accctgttcc atctgttcct gaccttgatc
     6001 tgaacttctc tattctcagt tatgtatttt tccatgcctt gcaaaatggc gttacttaag
     6061 ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata
     6121 aaatctttta ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag
     6181 tcaaaactag agcctggacc actgatatct cgaggtcgac ggtatcgatg cggggaggcg
     6241 gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga
     6301 gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag
     6361 aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat
     6421 gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag
     6481 cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc
     6541 ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac
     6601 acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc
     6661 aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac
     6721 aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca
     6781 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
     6841 tctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga
     6901 tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg
     6961 aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg
     7021 ccaggtatct ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa
     7081 aaatcccttc cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat
     7141 tttttctgac ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa
     7201 tccatcgctc gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc
     7261 taaccagcgt tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga
     7321 tatctttaac cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc
     7381 ccagaaatgc cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa
     7441 cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa
     7501 cgttttcttt tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg
     7561 gcagcccgga ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta
     7621 ctgccagacc gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg
     7681 gaaagcattt ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa
     7741 gcattttcca ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct
     7801 tgcgaacctc atcactcgtt gcatcgaccg gtaatgcagg caaattttgg tgtacggtca
     7861 gtaaattgga tgtactaggg ccgggattct cctccacgtc accgcatgtt agaagacttc
     7921 ctctgccctc cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
     7981 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
     8041 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
     8101 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
     8161 cgtcatcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta
     8221 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
     8281 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
     8341 gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt
     8401 gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg
     8461 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
     8521 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
     8581 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgg
     8641 tggcgggatc ctctagagtc gacctgcagc ccaagcttcg tctaacaaaa aagccaaaaa
     8701 cggccagaat ttagcggaca atttacta
//
