LOCUS       sequence-424582-        5766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR37. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_WDR37:1-494
FEATURES             Location/Qualifiers
     source          1..5766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2829..2950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2840..2859)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3108..3155
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3187..3981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3241..3260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3851..3870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4139..4727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4219..4238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4853..4901
                     /note="synthetic polyadenylation signal"
     CDS             complement(4925..5323)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5320..5329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5354..5711)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5368..5503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5520..5711
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5668..5688
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgcccacaga
     1261 aagcgcaagt tgttcgactg ctcgccaaac aaaacagaag cgcaaatccc atagcctttc
     1321 tatacgaaga actaacagct cggagcagga gaggacggga ctgccaagag acatgttaga
     1381 aggacaagat tctaaactgc cttcctcggt tcgcagtaca cttctggaac tgtttggtca
     1441 aatagaaaga gaatttgaaa acctttatat cgaaaactta gaattacgta gagaaatcga
     1501 cactcttaat gaacgtttag ctgctgaagg acaagcgatt gatggagcag agctgagtaa
     1561 gggccaactc aaaacaaaag ccagtcacag caccagccag ctctcccaga aactgaagac
     1621 cacttacaag gcttccacca gcaagattgt ctccagcttt aagaccacga catcgagagc
     1681 tgcctgccag ctcgtgaagg agtacatcgg ccaccgggac ggcatctggg atgtcagcgt
     1741 ggccaagaca cagccagtgg tgctcgggac tgcatcagcc gatcacacgg ctttgctgtg
     1801 gagcatagag acagggaagt gcctagtcaa gtacgcaggc cacgtgggct cagtaaattc
     1861 tatcaaattt catccctcag agcagttggc tctcactgct tctggagatc agactgctca
     1921 tatctggaga tacgcggtgc agctgccgac accccagcct gttgctgaca ctagtatatc
     1981 tggggaagat gaagtagagt gctctgacaa ggacgagccc gacctcgatg gggatgtgtc
     2041 cagcgactgc cccaccatcc gcgtcccact gacatccctc aagagccacc agggcgtggt
     2101 catcgcctcc gactggctgg ttggggggaa gcaggctgtg actgcctcct gggaccggac
     2161 ggcaaacctg tacgacgtgg agacgtccga gctcgttcac tctctgacag ggcacgacca
     2221 ggagttgacg cactgctgca cacaccccac ccagcggctc gtggtgacct cctcccgtga
     2281 cacgactttc cgcctctggg atttcaggga cccctccatc cactcggtga atgtttttca
     2341 gggacacacg gacactgtga cttctgccgt gttcaccgtg ggagacaacg tggtttcagg
     2401 cagcgatgac cgcacggtga aagtctggga cttgaaaaat atgagatccc ccattgcaac
     2461 tattcgcacg gactctgcca ttaacaggat caatgtatgt gtcggccaaa aaatcatagc
     2521 cctcccccat gacaaccgac aagtgagact gtttgatatg tcaggagtgc gcctggcgcg
     2581 gcttccccgg agcagccgac agggccacag gagaatggta tgctgctcgg catggagtga
     2641 agaccacccc gtgtgcaatc tgttcacctg tgggtttgac cggcaagcca ttggttggaa
     2701 catcaacatc cctgcattgc tacaagaaaa ataatctaga ggtcaagctt cgggggttta
     2761 aacctgcagg catgcaagct gatttggctg ctgccaccgc tgagcaaggc cgcttcgagc
     2821 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2881 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2941 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     3001 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcttaa ttaagacttc
     3061 taatataact tcgtatagca tacattatac kragwkatss yyycaytgtt gacaattaat
     3121 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa acccaggagg
     3181 cagatcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3361 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3961 cttcttgacg agttcttctg attcgaaatg accgaccaag cgacgcccaa ccggtatcag
     4021 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4561 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atttcaagaa gatcctttga
     4741 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4801 tgagattatc aaaaaggatc ttcacctaga tccttttata gtccggaatc gtcacacaaa
     4861 aaaccaacac acagatgtaa tgaaaataaa gatattttat tgcggccatc gtgatggcta
     4921 gagtttagcc ctcccacaca taaccagagg gcagcaattc acgaatccca actgccgtcg
     4981 gctgtccatc actgtccttc actatggctt tgatcccagg atgcagatcg agaagcacct
     5041 gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc gcgacgatac
     5101 aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt tctgcacaag
     5161 gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg ctagagagag
     5221 ctgcgctggc gacgctgtag tcttcagaga tggggatgct gttgattgta gccgttgctc
     5281 tttcaatgag ggtggattct tcttgagaca aaggcttggc catggtggcg ctagtgtcag
     5341 aagaatcgag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     5401 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     5461 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     5521 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     5581 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     5641 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
     5701 ttccgcctca gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg
     5761 ctgcgc
//
