LOCUS       sequence-424642-        6115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of RAB14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Tu X, Chu TT, Jeltema D, Abbott K, Yang K, Xing C, Han J, Dobbs N, 
            Yan N
  TITLE     Interruption of post-Golgi STING trafficking activates tonic 
            interferon signaling.
  JOURNAL   Nat Commun. 2022 Nov 15;13(1):6977. doi: 10.1038/s41467-022-33765-0.
  PUBMED    36379959
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-15"; volume: "13(1):6977. doi"; pages: " 
            10.1038/s41467-022-33765-0"
COMMENT     Sequence Label: pcDNA3.1-mRAB14-HA
FEATURES             Location/Qualifiers
     source          1..6115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(210..1001,1002..1070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     833..852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1071..1175)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1250..1269)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1441..1820
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1821..2024
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1975..1995
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2069..2088
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2069..2087
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2812..2838
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2912..2929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2918..3142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3188..3616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3275..3294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3485..3506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3625..3645)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3630..3959
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3810..3945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3872..3891
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4026..4820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4080..4099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4690..4709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4994..5115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5031..5050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5085..5104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5164..5180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5164..5180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5177..5199)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5188..5204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5212..5218,5219..5236,5237..5242))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5257..5278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5395..5412)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5566..6115,1..39))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5646..5665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
       61 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      121 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1141 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1201 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1261 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1321 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1381 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1441 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1501 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1561 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1621 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1681 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1741 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     1801 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     1861 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     1921 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     1981 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     2041 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
     2101 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
     2161 ttcaccatgg caactgcacc gtacaactac tcttacatct ttaagtatat tattattggg
     2221 gatatgggag taggaaaatc ttgcttgctt catcaattta cagaaaaaaa atttatggct
     2281 gattgtcctc acacaattgg tgttgaattt ggtacaagaa taattgaagt tagtggtcaa
     2341 aaaatcaaac tgcagatttg ggatacagca gggcaggagc ggttcagagc ggttacacgg
     2401 agctactata gaggagctgc aggtgcgctc atggtgtatg acatcaccag aagaagtaca
     2461 tataaccact taagcagctg gttgacagac gcaaggaatc tcaccaatcc aaacactgta
     2521 ataattctca taggaaataa agcagacttg gaagcacaga gagatgttac ctatgaagaa
     2581 gccaaacagt ttgctgagga aaacggctta ttgttccttg aagcaagcgc aaaaacggga
     2641 gagaatgtag aagatgcttt tcttgaggct gccaagaaaa tctatcagaa cattcaggat
     2701 ggaagcctgg atctgaatgc tgccgagtct ggtgtacaac acaaaccctc agccccgcag
     2761 ggaggacggc taaccagtga accccaaccc cagagagaag gctgtggctg ctacccatac
     2821 gacgtaccag attacgctta agaattctgc agatatccag cacagtggcg gccgctcgag
     2881 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     2941 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3001 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3061 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3121 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3181 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3541 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3601 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3661 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3721 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3781 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3841 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3961 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4201 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4321 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4801 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4861 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4921 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4981 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5101 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5161 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5221 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5281 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6001 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtt
//
