LOCUS       sequence-42466-e        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Reijmers LG, Perkins BL, Matsuo N, Mayford M
  TITLE     Localization of a stable neural correlate of associative memory.
  JOURNAL   Science. 2007 Aug 31;317(5842):1230-3.
  PUBMED    17761885
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-08-31"; volume: "317(5842)"; pages: "1230-3"
COMMENT     Sequence Label: pTRE_tau-LacZ::tTA-H100Y
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1219..4266
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(1221..1243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(4322..4340)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     intron          4424..4489
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4619..4639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4911..5045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4948..4967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5002..5021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5475..5492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5646..6234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5726..5745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6405..7196,7197..7265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7028..7047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7266..7370)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7396..7851)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7533..7554)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7745..7764
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7983..8207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8196..8213
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8233..8251
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(8442..8465)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    join(9212..9230,9231..9253,9254..9272,9273..9295,9296..9314,
                     9315..9337,9338..9356,9357..9379,9380..9398,9399..9421,
                     9422..9440,9441..9463,9464..9482)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9515..9553
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9550..9574
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          9708..9937
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     9920..9939
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg ctgagccccg ccaggagttc gacgtgatgg aagatcatgc tcagggggac
       61 tacaccctgc aagaccagga gggtgacatg gaccccggcc tgaaagagtc tcccctgcag
      121 accccggccg atgatggatc tgaggaacca ggctctgaaa cctctgatgc taagagcact
      181 ccgacggcgg aagatgcgac agcaccctta gtggatgagg gagcccccgg tgagcaggcg
      241 gccgctcagg cccccgcgga gatcccagaa ggaaccgcag ctgaagaagc aggcattggc
      301 gacacgtcca acctggaaga ccaagctgcc ggacacgtga cccaagctcg catggtcagt
      361 aaaggcaaag atgggactgg acccgatgac aaaaaaacca agggggcgga tggtaagcct
      421 ggaacgaaga ttgccacacc ccggggagca gcccctccag gccagaaagg ccaggccaac
      481 gccacccgga ttccagcaaa aaccactccc accccgaaga cctcgccagc aaccatgcaa
      541 gtgcagaaaa aaccaccccc tgcaggggca aaatctgaga gaggtgaatc tgggaaatcc
      601 ggggaccgca gcggctacag cagccccggc tccccaggca ctccgggcag ccgctcccgc
      661 acaccctccc tgccgacccc gcccacccgg gagcccaaga aggtggcggt ggtccgcact
      721 ccccccaagt cgccgtctgc agccaagagc cgcctgcagg ccgctcccgg gcccatgcca
      781 gacctgaaga acgtcaagtc caaaatcggc tccacggaaa acctgaagca ccagccagga
      841 ggtggcaagg tgcagataat taataagaag ctggatctta gcaacgtcca gtccaagtgt
      901 ggctcaaagg ataatatcaa acacgtgcca ggaggcggca gtgtgcaaat agtctacaaa
      961 ccagtggatc tgagcaaggt gacctccaag tgtggctcat taggcaacat ccatcataag
     1021 ccaggaggtg gccaagtgga agtgaaatct gagaagctgg acttcaagga tagagtccag
     1081 tcgaagattg ggtccctgga caacatcaca cacgtccctg gcggagggaa taaaaagatc
     1141 gaaacccaca agctgacctt ccgcgagaac gccaaagcca agaccgacca cggggcggag
     1201 atcgtgtccg gggatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1261 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1321 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg
     1381 tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat
     1441 actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac
     1501 gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt
     1561 tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt
     1621 tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc
     1681 caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac
     1741 cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg
     1801 tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc
     1861 agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct
     1921 gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag
     1981 ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt
     2041 ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc
     2101 gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg
     2161 attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg
     2221 ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg
     2281 catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac
     2341 aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc
     2401 gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca
     2461 atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga
     2521 atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca
     2581 ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc
     2641 ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg
     2701 tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa
     2761 tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg
     2821 ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta
     2881 cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc
     2941 aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt
     3001 atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac
     3061 cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac
     3121 ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg
     3181 ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg
     3241 cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa
     3301 ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg
     3361 gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc
     3421 agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca
     3481 ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat
     3541 cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac
     3601 cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg
     3661 ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg
     3721 cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt
     3781 caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt
     3841 ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg
     3901 caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg
     3961 tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc
     4021 gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac
     4081 agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca
     4141 tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca
     4201 gtatcggcgg aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa
     4261 aaataaatcc atccatcaca ctggcggccg gccgctcgag catgcatcta gagggcccta
     4321 ttctatagtg tcacctaaat gctagaggat ctttgtgaag gaaccttact tctgtggtgt
     4381 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     4441 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     4501 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     4561 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     4621 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     4681 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     4741 aaaagctgca ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac
     4801 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4861 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5041 ggatcatccc gccatggtat caacgccata tttctattta cagtagggac ctcttcgttg
     5101 tgtaggtacc gctgtattcc tagggaaata gtagaggcac cttgaactgt ctgcatcagc
     5161 catatagccc ccgctgttcg acttacaaac acaggcacag tactgacaaa cccatacacc
     5221 tcctctgaaa tacccatagt tgctagggct gtctccgaac tcattacacc ctccaaagtc
     5281 agagctgtaa tttcgccatc aagggcagcg agggcttctc cagataaaat agcttctgcc
     5341 gagagtcccg taagggtaga cacttcagct aatccctcga gccgcggccg atttaaatgt
     5401 ttaaacgggc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5461 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5521 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5581 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6061 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6361 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6481 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6541 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6601 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6721 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6781 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6841 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6901 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7021 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7081 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7141 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7201 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7261 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7321 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7381 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7441 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7501 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7561 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7621 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7681 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     7741 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     7801 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     7861 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7921 agggggttta aacatttaaa ttcgaaccct cgagccccag ctggttcttt ccgcctcaga
     7981 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
     8041 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     8101 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     8161 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
     8221 gcgagctcta gcatttaggt gacactatag aatagggccc accggttact agatgcatgc
     8281 tcgagcggcc ggccgccagt gtgatggatg gatgatcctt atcacagcat gtccaggtcg
     8341 aagtcgtcca gggcgtcggc cggcagcatg tccaggtcga agtcgtccag ggcgtcggcc
     8401 ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggccg gcttgtcgtc gtcgtccttg
     8461 tagtcgaccc ttgcgcttct tcttggggga gccggactcg cacttcagct gcttctccag
     8521 gccgcagatg atcagctcca ggccgaacag gaaggcgggc tcggcgccct ggtggtcgaa
     8581 cagctcgatg gcctggcgca gcaggggggg catggagtcg gtggtggggg tctcgcgctc
     8641 ctccttggcc acctggtgct cctggtcctc cagcacgcag cccagggtga agtggcccac
     8701 ggcggacagg gcgtacaggg cgttctccag ggagaagccc tgctggcaca ggaaggccag
     8761 ctggttctcc agggtctcgt actgcttctc ggtggggcgg gtgcccaggt acaccttggc
     8821 gccgtcgcgg tgggacagca gtgcgcagcg gaaggacttg gcgttgttgc gcaggaagtc
     8881 ctgccaggac tcgccctcca gggggcagaa gtgggtgtgg tggcggtcca gcatctcgat
     8941 ggccagggcg tccagcaggg cgcgcttgtt cttcacgtgc cagtacaggg tgggctgctc
     9001 cacgcccagc ttctgggcca gcttgcgggt ggtcaggccc tcgatgccca cctcgttcag
     9061 cagctccagg gcggagttga tcaccttgga cttgtccagt cgcgacataa gctagcgtgt
     9121 tcgaggccac acgcgtcacc ttaatatgcg aagtggacct gggaccgcgc cgccccgact
     9181 gtacacggcc gccaactctc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     9241 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     9301 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     9361 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     9421 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     9481 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     9541 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     9601 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tcaccacgtg
     9661 gccgcatcga ttctagaatt cgctgtctgc gagggccagc tgttggggtg agtactccct
     9721 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
     9781 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa
     9841 gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg
     9901 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     9961 cagcccaagc ggatcctgcg gat
//
