LOCUS       sequence-424915-        3245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of Ypet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM8734
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             478..1191
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     terminator      1217..1303
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1340..1357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1553..1572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2056..2150)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2180..2971,2972..3040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2803..2822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3041..3145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3213..3231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
      541 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac gtacggtaaa
      601 ttgaccttaa aattactctg tactactggt aaattgccag ttccatggcc aaccttagtc
      661 actactttag gttatggtgt tcaatgtttt gctagatacc cagatcatat gaaacaacat
      721 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
      781 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
      841 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
      901 gaatacaact ataactctca caatgtttac atcactgctg acaaacaaaa gaatggtatc
      961 aaagctaact tcaaaattag acacaacatt gaagatggtg gtgttcaatt agctgaccat
     1021 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
     1081 tcctatcaat ctgccttatt caaagatcca aacgaaaaga gagaccacat ggtcttgtta
     1141 gaatttttga ctgctgctgg tattaccgag ggtatgaatg aattgtacaa atgataagcg
     1201 gccgctctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1261 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta
     1321 ggcggagata ttccgcttcc tcgctcactg actcgctccg ctcggtcgtt cggctgcggc
     1381 gagcggtacc ggcttactgg cggggcggaa atttcctgga agatgccagg aagaaactta
     1441 acagggaagc gataaggcca cggcagagcc gtttttccat aggctccgcc cccctgacaa
     1501 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac ttaaagatac
     1561 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1621 ggtgtcactc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcggttccgg
     1681 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg
     1741 cgcctgttcc ggtaactatc gacttgagtc caagccggaa agacacgaca aagcgccact
     1801 ggcagcagcc actggtaaga gggtgtacaa gagattactg atgcagagtt cttgaagtca
     1861 gagccaaact acggctaaac tggaaggacg aatttggtgg cagcggtctt gtacaccctg
     1921 ttaccacggt tcagaagttc cccaacttac tgaaccttcg aaaaaccacc tccccaggtg
     1981 gttttttcgt tttcagagca agagattacg acgcaacgga aaggatctca agaggttcat
     2041 cttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2101 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2161 cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3181 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     3241 ttcac
//
