LOCUS       sequence-42497-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RAB3B (3DZ8)
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             638..655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca attttgacta catgtttaaa ctgcttatca
      121 ttggcaacag cagtgttggc aagacctcct tcctcttccg ctatgctgat gacacgttca
      181 ccccagcctt cgttagcacc gtgggcatcg acttcaaggt gaagacagtc taccgtcacg
      241 agaagcgggt gaaactgcag atctgggaca cagctgggca ggagcggtac cggaccatca
      301 caacagccta ttaccgtggg gccatgggct tcattctgat gtatgacatc accaatgaag
      361 agtccttcaa tgctgtccaa gactgggcta ctcagatcaa gacctactcc tgggacaatg
      421 cacaagttat tctggtgggg aacaagtgtg acatggagga agagagggtt gttcccactg
      481 agaagggcca gctccttgca gagcagcttg ggtttgattt ctttgaagcc agtgcaaagg
      541 agaacatcag tgtaaggcag gcctttgagc gcctggtgga tgccatttgt gacaagatgt
      601 ctgattcgtg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      661 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc
//
