LOCUS       sequence-424975-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector control - contitutive expression of mRuby33 alone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Sweeney KM, Chantarawong S, Barbieri EM, Cajka G, Liu M, Spruce L, 
            Fazelinia H, Portz B, Copley K, Lapidot T, Duhamel L, Greenwald P, 
            Saida N, Shalgi R, Shorter J, Shalem O
  TITLE     CRISPR screen for protein inclusion formation uncovers a role for 
            SRRD in the regulation of intermediate filament dynamics and 
            aggresome assembly.
  JOURNAL   PLoS Genet. 2024 Feb 5;20(2):e1011138. doi: 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb.
  PUBMED    38315730
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2024-02-5"; volume: "20(2):e1011138. doi"; pages: " 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb"
COMMENT     Sequence Label: TRE-mRuby3
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..76
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(195..215)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        200..529
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      380..515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     442..461
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        577..624
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             643..1017
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1147..1268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1184..1203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1238..1257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1317..1333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1317..1333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1330..1352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1341..1357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1365..1371,1372..1389,1390..1395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1410..1431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1548..1565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1719..2307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1799..1818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2478..3269,3270..3338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3101..3120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3339..3443)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3518..3537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3709..4088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4090..4288
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4306..4486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4533..4658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5151..5384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5569..5613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5911..6028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6123..6437
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    6131..6149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6167..6185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6202..6220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6238..6256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6274..6292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6309..6327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6345..6363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6410..6434
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6454..7164
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     CDS             7214..7270
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7271..8071
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7331..7350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7941..7960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        8127..8338
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(8363..8980,8981..8986,8987..9022,9023..9025,9026..9061,
                     9062..9064,9065..9100,9101..9109)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9149..9169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9567..9578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9783..10016
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10042..10059)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10048..10272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10318..10560,1..186)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10405..10424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
       61 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     1021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3481 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3541 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3601 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3661 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3721 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3781 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3841 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3901 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3961 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4021 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4081 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4141 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4201 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4261 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     6061 ttggttaatc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     6121 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     6181 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     6241 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     6301 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     6361 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     6421 tgaaccgtca gatcgcctgg agaattggca ccgatggtgt ctaagggcga agagctgatc
     6481 aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa
     6541 tgcacaggtg aaggagaagg cagaccgtac gagggagtgc aaaccatgag gatcaaagtc
     6601 atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt catgtatggc
     6661 agccgtacct ttatcaagta cccggccgac atccctgatt tctttaaaca gtcctttcct
     6721 gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt caccgtcacg
     6781 caggacacca gccttgagga tggcgagctc gtctacaacg tcaaggtcag aggggtaaac
     6841 tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc taatacagag
     6901 atgatgtatc cagcagatgg tggtctgaga ggatacactg acatcgcact gaaagttgat
     6961 ggtggtggcc atctgcactg caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg
     7021 aacatcaaga tgcccggtgt ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt
     7081 gacaatgaaa cctacgtagt gcaaagagaa gtggcagttg ccaaatacag caaccttggt
     7141 ggtggcatgg acgagctgta caagggatcc ggaagtggct caagcggagg agacggacgt
     7201 ctctggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     7261 tcctggaccg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7321 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7381 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7441 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7501 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7561 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7621 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7681 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7741 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7801 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7861 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7921 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7981 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8041 cttctatcgc cttcttgacg agttcttctg agaattcgct agctaggtct tgaaaggagt
     8101 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     8161 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     8221 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     8281 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
     8341 ccggttctag agcgctgcca ccatgtctag actggacaag agcaaagtca taaacggagc
     8401 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     8461 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     8521 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     8581 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     8641 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     8701 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     8761 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     8821 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     8881 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     8941 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     9001 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     9061 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     9121 tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9181 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9241 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9301 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9361 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9421 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9481 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     9541 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9601 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     9661 tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt
     9721 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     9781 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     9841 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9901 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9961 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
    10021 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    10081 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    10141 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    10201 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    10261 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
    10321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    10381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    10441 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    10501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
//
