LOCUS       sequence-425007-       13224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A
            cDNA in mammilian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Klomp JE, Diehl JN, Klomp JA, Edwards AC, Yang R, Morales AJ, Taylor
            KE, Drizyte-Miller K, Bryant KL, Schaefer A, Johnson JL, Huntsman 
            EM, Yaron TM, Pierobon M, Baldelli E, Prevatte AW, Barker NK, 
            Herring LE, Petricoin EF 3rd, Graves LM, Cantley LC, Cox AD, Der CJ,
            Stalnecker CA
  TITLE     Determining the ERK-regulated phosphoproteome driving KRAS-mutant 
            cancer.
  JOURNAL   Science. 2024 Jun 7;384(6700):eadk0850. doi: 
            10.1126/science.adk0850. Epub 2024 Jun 7.
  PUBMED    38843329
REFERENCE   2  (bases 1 to 13224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-7"; volume: "384(6700):eadk0850. doi"; pages: " 
            10.1126/science.adk0850. Epub 2024 Jun 7"
COMMENT     Sequence Label: pInducer-myc-ERK2-R67S/S153D/Y263A
FEATURES             Location/Qualifiers
     source          1..13224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2375..2404
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3461..3496
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     promoter        3513..4724
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4561..4580
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4731..4764
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4771..5388,5389..5394,5395..5430,5431..5433,5434..5469,
                     5470..5472,5473..5508,5509..5517)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5566..6138
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5733..5750)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5960..5979
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6141..6743
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6640..6660
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6765..7353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6818..6838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7236..7247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7560..7793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7894..7911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7900..8124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8170..8598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8257..8276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8467..8488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8607..8627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8612..8941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8792..8927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8854..8873
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9764..9885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9934..9950)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9934..9950)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9947..9969)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9958..9974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9982..9988,9989..10006,10007..10012))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10027..10048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10165..10182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10336..10924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10416..10435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11095..11886,11887..11955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11718..11737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11956..12060)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12108..12242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12410..13043
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13090..13215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tatggagcag aagctgatct ccgaggagga
     2401 cctgggatct gggcatatgg cggcggcggc ggcggcgggc gcgggcccgg agatggtccg
     2461 cgggcaggtg ttcgacgtgg ggccgcgcta caccaacctc tcgtacatcg gcgagggcgc
     2521 ctacggcatg gtgtgctctg cttatgataa tgtcaacaaa gttcgagtag ctatcaagaa
     2581 aatcagcccc tttgagcacc agacctactg ccagagtacc ctgagggaga taaaaatctt
     2641 actgcgcttc agacatgaga acatcattgg aatcaatgac attattcgag caccaaccat
     2701 cgagcaaatg aaagatgtat atatagtaca ggacctcatg gaaacagatc tttacaagct
     2761 cttgaagaca caacacctca gcaatgacca tatctgctat tttctctacc agatcctcag
     2821 agggttaaaa tatatccatt cagctaacgt tctgcaccgt gacctcaagc ctgacaacct
     2881 gctgctcaac accacctgtg atctcaagat ctgtgacttt ggcctggccc gtgttgcaga
     2941 tccagaccat gatcacacag ggttcctgac agaatatgtg gccacacgtt ggtacagggc
     3001 tccagaaatt atgttgaatt ccaagggcta caccaagtcc attgatattt ggtctgtagg
     3061 ctgcattctg gcagaaatgc tttctaacag gcccatcttt ccagggaagc attatcttga
     3121 ccagctgaac cacattttgg gtattcttgg atccccatca caagaagacc tgaattgtat
     3181 aataaattta aaagctagga acgctttgct ttctcttcca cacaaaaata aggtgccatg
     3241 gaacaggctg ttcccaaatg ctgactccaa agctctggac ttattggaca aaatgttgac
     3301 attcaaccca cacaagagga ttgaagtaga acaggctctg gcccacccat atctggagca
     3361 gtattacgac ccgagtgacg agcccatcgc cgaagcacca ttcaagttcg acatggaatt
     3421 ggatgacttg cctaaggaaa agctcaaaga actaattttt gaagagactg ctagattcca
     3481 gccaggatac agatcttaaa cgcgtaagat ctggcctccg cgccgggttt tggcgcctcc
     3541 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     3601 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     3661 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     3721 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     3781 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     3841 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     3901 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     3961 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     4021 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     4081 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     4141 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     4201 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     4261 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     4321 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     4381 atgcagggtg gggccacctg ccggtaggtg tgtggtaggc ttttctccgt cgcaggacgc
     4441 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     4501 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     4561 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     4621 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     4681 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacggatcc ataacttcgt
     4741 ataatgtatg ctatacgaag ttatgccacc atgtctagac tggacaagag caaagtcata
     4801 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     4861 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     4921 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     4981 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     5041 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     5101 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     5161 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     5221 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     5281 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     5341 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     5401 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     5461 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaata
     5521 acttcgtata gcatacatta tacgaagtat ggcgcgccaa ttccgcccct ctccctcccc
     5581 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5641 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5701 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5761 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5821 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5881 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5941 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6001 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6061 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6121 ttgaaaaaca cgataatacc atggccaccg agtacaagcc cacggtgcgc ctcgccaccc
     6181 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6241 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6301 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6361 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggct
     6421 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6481 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6541 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6601 gcgctggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6661 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6721 tgacccgcaa gcccggtgcc tgattaatta agcgcgtctg gaacaatcaa cctctggatt
     6781 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6841 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6901 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6961 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7021 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7081 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7141 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7201 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7261 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7321 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     7381 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     7441 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     7501 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     7561 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7801 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7861 gaggccttga cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7921 catctgttgt ttgccccttc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7981 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     8041 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     8101 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     8161 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8221 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8281 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8341 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8401 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8461 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8521 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8581 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8881 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8941 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc
     9001 tgaactcacc gcgacgtgac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     9061 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     9121 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     9181 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     9241 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     9301 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     9361 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     9421 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     9481 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     9541 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     9601 acgccccagc actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac
     9661 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     9721 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     9781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9841 actgcattct agttgtggtt tgtccaaact aatcaatgta tcttatcatg tctgtatacc
     9901 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9961 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10021 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    10141 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10561 gtaggtcgtt cgctccaagc tgggctgtgt gcactaaccc cccgttcagc ccgaccgctg
    10621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    10801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12061 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
    12121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    12181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12241 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12301 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12361 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    12421 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    12481 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    12541 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    12601 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    12661 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    12721 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    12781 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    12841 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    12901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    13021 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    13081 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    13141 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    13201 gggtgcgaga gcgtcagtat taag
//
