LOCUS       sequence-425059-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO2s
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..582
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     483..502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     699..716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     849..865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     849..865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1188..1205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1188..1204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1523..2314
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAAYWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2315..2334
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2315..2333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2422..2440)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatatacac
      841 tccgctagca ggaaacagct atgaccatac ccgggtaagt gactagcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggctccga agagccgagt
      961 gtcttcggtt cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg
     1021 cgtactagtc tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc
     1081 tgagagaaga cacgtcgctc ttcgacgtaa gtaactgact cggtaccaaa ttccagaaaa
     1141 gaggcctccc gaaagggggg ccttttttcg ttttggtcct aagcttaact ggccgtcgtt
     1201 ttacaccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     1261 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     1321 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1381 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1441 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1501 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1561 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1621 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1681 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1741 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1801 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1861 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1921 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1981 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     2041 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2101 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgcct actgggcaat
     2161 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     2221 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     2281 gaaaggcgag atcaccaagg tagtcggcaa ataataatac gactcactat agggtaacta
     2341 attgaggcgc gcctaccacc gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt
     2401 caaccttcct aggtaagtga ccctttagtg agggttaatt
//
