LOCUS       sequence-425184-        5073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of iCre in striatal Pvalb neurons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP13450: pAAV-AiE0140h-minBG-iCre-4X2C-WPRE3-...
FEATURES             Location/Qualifiers
     source          1..5073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             544..564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1900..1920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2107..2218
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2336..2476
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2336..2465
                     /label=AAV2 ITR
     rep_origin      2551..3006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2638..2657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2848..2869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3023..3042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3142..3164
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3202..3220)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3288..3392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3393..3461,3462..4253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3611..3630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4424..5012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4913..4932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggttgt gtgtgaagat taagcccttt cttgctctat
      181 gtatttgagc ctagaaaaag acacactccc aagaaattag aataaaggga aaaatgactt
      241 tgagatgtac tttcttaaaa taactgaagt aatgaccgca tgagccagct gatgggtttt
      301 taaatggata cgtttctatc agcctgctgc ttgatgccag agccaaatat atatggagtg
      361 tattatatca tatcctgtag gcaggagact gttggggcat tgggggactt agagaggtga
      421 agtggcaggc ttggcacagg aattaacagc agagctcggg ctgggcataa aagtcagggc
      481 agagccatct attgcttaca tttgcttctg ggatccagat ctttcgaagc tagcaccatg
      541 gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa cctgcctgcc
      601 ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac
      661 aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct
      721 gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga tgtgagggac
      781 tacctcctgt acctgcaagc cagaggcctg gctgtgaaga ccatccaaca gcacctgggc
      841 cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc caatgctgtg
      901 tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag agccaagcag
      961 gccctggcct ttgaacgcac tgactttgac caagtcagat ccctgatgga gaactctgac
     1021 agatgccagg acatcaggaa cctggccttc ctgggcattg cctacaacac cctgctgcgc
     1081 attgccgaaa ttgccagaat cagagtgaag gacatctccc gcaccgatgg tgggagaatg
     1141 ctgatccaca ttggcaggac caagaccctg gtgtccacag ctggtgtgga gaaggccctg
     1201 tccctggggg ttaccaagct ggtggagaga tggatctctg tgtctggtgt ggctgatgac
     1261 cccaacaact acctgttctg ccgggtcaga aagaatggtg tggctgcccc ttctgccacc
     1321 tcccaactgt ccacccgggc cctggaaggg atctttgagg ccacccaccg cctgatctat
     1381 ggtgccaagg atgactctgg gcagagatac ctggcctggt ctggccactc tgccagagtg
     1441 ggtgctgcca gggacatggc cagggctggt gtgtccatcc ctgaaatcat gcaggctggt
     1501 ggctggacca atgtgaacat tgtgatgaac tacatcagaa acctggactc tgagactggg
     1561 gccatggtga ggctgctcga ggatggggac taatgaggcg cgccgcggcc ttaaagagac
     1621 cggttcactg tgacagtaaa agagaccggt tcactgtgag aatgaaagag accggttcac
     1681 tgtgatcgga aaagagaccg gttcactgtg agcggccttg aaacccagca gacaatgtag
     1741 ctcagtagaa acccagcaga caatgtagct gaatggaaac ccagcagaca atgtagcttc
     1801 ggagaaaccc agcagacaat gtagctgtcg acgaattcga tatcataatc aacctctgga
     1861 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     1921 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     1981 ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct
     2041 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg ctcgagagat
     2101 cttcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2161 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2221 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2281 aggattggga agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa
     2341 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     2401 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     2461 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2521 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     2581 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     2641 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2701 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2761 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2821 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2881 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     2941 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     3001 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3061 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3121 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3181 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3241 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3361 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3421 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3481 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3541 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3601 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3661 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3721 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3781 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3841 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3901 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3961 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4021 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4081 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4141 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4201 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4261 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4321 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4381 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4561 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5041 tcctggcctt ttgctggcct tttgctcaca tgt
//
