LOCUS       sequence-42570-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, 
            with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..76)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(87..106)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          89..221
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 caattcgggt cgatggtgaa gcattggtct tcctattttg atataacttc gtataggata
       61 ctttatacga agttatgggg atcatcacct gtggagagaa aggcaaagtg gatgtcagta
      121 agaccaatag gtgcctatca gaaacgcaag agtcttctct gtctcgacaa gcccagtttc
      181 tattggtctc cttaaacctg tcttgtaacc ttgatactta cctgatccaa cggctgcgac
      241 ttggggcagg gggtcctagg ggtggtctgg ccaggagccg cgggggcgga ctgcgcggtg
      301 cccaggagca cagctgcgct ctcaggcacg acacgactcc tccgctgccc accgcagact
      361 gaggcagcgc tgagtcgccg gcgccgcagc gcagatggtc gcgcccgtgc ccccctatct
      421 cgcgcctcgc gtggtgcggt ccggctgggc cggcggcggc gcggacgcga ccaaggtggc
      481 cgggaagggg agtttgcggg ggaccggcga gtgacgtcag cgcgccttca gtgctgaggc
      541 ggcggtggcg cgcgccgcca ggcgggggcg aaggcactgt ccgcggtgct gaagctggca
      601 gtgcgcacgc gcctcgccgc atcctgtttc ccctccccct ctctgatagg ggatgcgcaa
      661 tttggggaat
//
