LOCUS       sequence-425937-        8808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human GEMIN5. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_GEMIN5:1-1508
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5871..5992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5882..5901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6107..6140)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6150..6197
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6229..7023
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6283..6302)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6893..6912
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(7181..7769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7261..7280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7895..7943
                     /note="synthetic polyadenylation signal"
     CDS             complement(7967..8365)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      8362..8371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8396..8753)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8410..8545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8562..8753
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8710..8730
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggggcagga
     1261 gccgcggacg ctgccgccct cccccaactg gtactgcgcc cgctgcagcg atgccgtgcc
     1321 cgggggcctc tttggcttcg ccgcgcggac ctccgtcttc cttgtccgcg tgggcccggg
     1381 cgcaggcgag agtccaggga cacccccgtt tcgagtcata ggagagttgg tgggacacac
     1441 cgaaagggtc tctggcttca cattttctca tcaccctggt cagtacaacc tctgtgccac
     1501 cagctccgac gatgggactg tgaaaatatg ggatgtagag acaaaaacag ttgtgacaga
     1561 acatgcactc catcagcata cgatatcaac attacattgg tctcctcgag taaaggactt
     1621 aatagtatct ggggatgaaa aaggagtagt tttctgttac tggtttaaca gaaatgacag
     1681 ccagcacctc tttatagaac ccaggacaat tttctgtctt acttgttcac ctcatcatga
     1741 agatttagta gccattggct acaaggatgg catagtggtg ataattgaca tcagtaagaa
     1801 aggagaagtt attcataggc ttcgaggcca tgatgatgaa atccactcca tagcctggtg
     1861 tcccctgcct ggtgaagatt gtttatctat aaaccaagag gaaacttcag aagaagctga
     1921 aattaccaac gggaatgctg tagcacaagc tccagtaaca aaaggttgct acttagccac
     1981 tggaagcaaa gatcaaacca ttcgaatctg gagctgttct agaggccgag gggtgatgat
     2041 tttgaaattg ccctttctga agagaagagg agggggtata gacccaactg ttaaagagcg
     2101 cctttggttg acactccatt ggcccagcaa tcaaccaaca cagctggtat ctagctgttt
     2161 tggaggtgaa ctgttgcaat gggatctcac tcaatcttgg agacggaaat acaccctctt
     2221 cagtgcctca tcagaagggc aaaatcattc aagaattgtg tttaatttat gtcctttaca
     2281 aacagaggat gacaaacagc tgttactttc tacatcaatg gatagagatg taaaatgttg
     2341 ggacatagcc accttggagt gcagctggac ccttccttcc cttggtgggt ttgcatacag
     2401 cctggctttc tcttctgtgg acataggctc tttggccata ggtgttgggg atggcatgat
     2461 ccgtgtatgg aatacactct ccataaagaa caactatgat gtgaaaaatt tttggcaagg
     2521 cgtgaagtcc aaggttacag cgctgtgctg gcacccaacc aaggaaggtt gcttagcttt
     2581 tggaactgat gatggaaaag tgggattgta tgacacctac tccaacaagc ctccacagat
     2641 ttctagcaca tatcataaga agactgtata tacgttagcc tgggggccac cagtaccccc
     2701 catgtcactt ggaggagaag gagacagacc ttcccttgct ttatacagct gtggaggaga
     2761 agggattgtc ttacagcata atccctggaa gcttagtgga gaagcctttg acatcaacaa
     2821 actcatcagg gacaccaatt caatcaaata caaattgcct gtacacacag agataagttg
     2881 gaaagcagat ggcaaaatca tggctcttgg caatgaagat ggatcaatag aaatatttca
     2941 gattcccaac ctgaaactga tctgtactat ccaacagcat cacaagcttg tgaataccat
     3001 tagctggcat catgagcatg gcagccagcc agaattgagc tatctgatgg cctctggctc
     3061 caacaatgca gtcatttacg tgcacaacct gaagactgtc atagagagca gccctgagtc
     3121 tccagtgacc attacagagc cctaccggac cctctcaggg catacggcca agattaccag
     3181 tgtggcgtgg agcccacatc atgatggaag gctggtatct gcttcctatg atggtacagc
     3241 ccaggtgtgg gatgctctcc gggaagagcc cctgtgcaat ttccgaggac atcaaggtcg
     3301 actgctttgt gtggcatggt ctcctttgga tccagactgc atctattcag gggcagatga
     3361 cttttgtgtg cacaagtggc tcacttccat gcaagatcat tcccggcctc ctcaaggcaa
     3421 aaaaagtatt gaattggaga aaaaacggct ctctcaacct aaggcaaagc ccaaaaagaa
     3481 gaaaaagccc accttgagaa ctcctgtaaa gctggaatcg attgatggaa atgaagaaga
     3541 aagcatgaag gagaactcag gacctgttga gaatggtgtg tcagaccaag aaggggagga
     3601 gcaagcacgg gagccggaat taccctgtgg ccttgctcca gcggtttcta gagaaccagt
     3661 tatctgcact ccagtttcct caggctttga aaagtcaaaa gtcaccatta ataacaaagt
     3721 cattttactg aaaaaggagc caccaaaaga gaagccagaa accttaatca agaagagaaa
     3781 agctcgttcc ttgcttcccc tgagtacaag cctggaccac agatccaaag aggagcttca
     3841 tcaggactgt ttggtactag caactgcaaa gcactccaga gagctgaatg aagatgtgtc
     3901 tgctgatgtt gaggaaagat ttcatctggg gcttttcaca gacagggcta ccctgtatag
     3961 aatgattgat attgaaggaa aaggtcactt agaaaatggc caccctgagt tatttcacca
     4021 gcttatgctt tggaaaggag atctcaaagg tgttctccag actgcagcag aaagagggga
     4081 gctgacagac aaccttgtgg ctatggcacc agcagctggc taccatgtgt ggctatgggc
     4141 tgtggaagct tttgccaaac agctgtgttt tcaggatcag tatgtcaagg ctgcttctca
     4201 cctactttcc atccacaaag tgtatgaagc ggtggagctg ctcaagtcaa accattttta
     4261 cagggaagct attgcgattg ccaaggcccg gctgcgcccg gaggacccag tcctgaagga
     4321 cttgtacctc agctggggaa ccgtcctaga aagagatggc cactatgctg tagctgccaa
     4381 atgctattta ggggccactt gtgcttatga tgcagccaaa gttttggcca aaaaggggga
     4441 tgcggcatca cttagaacgg ctgcagagtt ggctgccatc gtaggagagg atgagttgtc
     4501 tgcttccctg gctctcagat gtgcccaaga gctgcttctg gccaacaact gggtgggagc
     4561 ccaggaagcc ctgcagctgc atgaaagtct acagggtcag agattggtgt tttgccttct
     4621 ggagctactg tccaggcatc tggaggaaaa gcagctttca gagggcaaaa gctcctcctc
     4681 ttaccacact tggaacacgg gcaccgaagg gcctttcgtg gagagggtga ctgcagtgtg
     4741 gaagagcatc ttcagccttg acacccctga gcagtatcag gaagcctttc agaagctgca
     4801 gaacatcaag tacccatctg ctacaaataa cacacctgcc aaacagctcc tgcttcacat
     4861 ttgccatgac ttgaccctgg cagtgctgag ccaacagatg gcctcctggg acgaggctgt
     4921 gcaggcgctc cttcgggcgg tggtccggag ctatgactca gggagcttca ccatcatgca
     4981 ggaagtgtac tcagcctttc tccctgatgg ctgtgaccac ctaagagaca agttggggga
     5041 ccatcaatcc cctgccacac cagctttcaa aagtttggag gccttttttc tttatgggcg
     5101 tctgtatgaa ttctggtggt ctctctccag accttgccca aattccagtg tctgggtaag
     5161 ggctggtcac agaacactct ctgttgagcc aagccagcag ttagacactg ccagcactga
     5221 agaaacggac cctgaaactt ctcagccaga gccaaacagg ccttcagaac tagacttgag
     5281 actcacagaa gaaggtgagc gaatgctgag tacttttaag gagctctttt cagaaaagca
     5341 tgccagtctc caaaactcac agagaactgt tgctgaagtc caagagacct tggcagaaat
     5401 gatccgacaa caccaaaaga gtcaactctg taaatccaca gcaaatggtc ctgataagaa
     5461 tgaaccggaa gtagaagcag agcagcccct ctgcagttct cagagccagt gtaaagaaga
     5521 aaaaaatgag ccactttctc tgcctgagtt aaccaaaagg cttaccgagg caaatcagag
     5581 aatggcaaaa tttcctgaga gcattaaggc ctggcccttc ccagatgtgc tggagtgctg
     5641 cctcgtcctg cttctcatca ggtcccactt tcctggctgt ctggcccagg aaatgcagca
     5701 gcaggcccaa gagctccttc agaaatacgg caacacgaaa acttacagaa gacactgcca
     5761 gaccttctgt atgtaatcta gaggtcaagc ttcgggggtt taaacctgca ggcatgcaag
     5821 ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga taagatacat
     5881 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5941 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     6001 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt ttttaagcaa
     6061 gtaaaacctc tacaaatgtg gtaaaatctt aattaagact tctaatataa cttcgtatag
     6121 catacattat acgaagttat ggttccactg ttgacaatta atcatcggca tagtatatcg
     6181 gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat gattgaacaa
     6241 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6301 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6361 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6421 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6481 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6541 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6601 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6661 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6721 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6781 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6841 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6901 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6961 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7021 tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa
     7081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7741 agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg
     7801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7861 tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac acacagatgt
     7921 aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag ccctcccaca
     7981 cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca tcactgtcct
     8041 tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg
     8101 ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg ttgccagctg
     8161 ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca gtaaaatgat
     8221 atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg gcgacgctgt
     8281 agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg agggtggatt
     8341 cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg agctttttgc
     8401 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     8461 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     8521 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     8581 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     8641 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     8701 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct cagaaggtac
     8761 ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgc
//
