LOCUS       sequence-42598-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, 
            with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    198..231
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttctcgag tggatcatct gcagaattcg
       61 cccttttcag tgtttcgaac ctcttcgagg gacataactt cgtataatgt atgctatacg
      121 aagttatggg tcgatggtga gatctggact agagggtcga tggtgatgct tggatcaaaa
      181 taggaagaat cccaatgata acttcgtata aagtatccta tacgaagtta tactaggggc
      241 tagcaagctt gaattcacca tgcgctgttc tccaggcggc gtgtggctcg ccctggctgc
      301 ttcccttctg cacgttagcc tgcagggtga gttccagcgc aaactgtata aggagcttgt
      361 taagaattat aaccccctgg agcggccggt cgcaaatgat tcccagccac tgacagtgta
      421 cttcagcctc tccttgctgc agatcatgga cgtggatgaa aagaaccagg tgctgaccac
      481 taatatttgg ttgcagatgt cctggaccga tcactacttg cagtggaatg tgagcgaata
      541 cccaggtgta aagactgtaa gattccctga cggccaaatc tggaaaccag atatcctgct
      601 gtacaacagc gcagacgaaa ggtttgatgc aacatttcac accaacgtgt tggtcaattc
      661 ttcaggccac tgccagtact tcccccctgg aatcttcaag tcctcatgct atatcgacgt
      721 ccgctggttt cccttcgacg tccagcactg caaactcaaa ttcgggagct ggagctacgg
      781 cggatggagc ctgg
//
