LOCUS       sequence-426082-        5771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human COP1 fragment A386 to V731. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_COP1:386-731
FEATURES             Location/Qualifiers
     source          1..5771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      957..1545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1446..1465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1699..1716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  1850..2074
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2141..2674)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2844..2849,2850..2866,2867..2872))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3224..3315
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3240..3257
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3374..3391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3404..3424
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    4600..4734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4637..4656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4691..4710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4763..4928
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5112..5566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5199..5218)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5408..5429
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5592..5696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5697..5765,5766..5771,1..786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
       61 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      121 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      181 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      241 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      301 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      361 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      421 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      481 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      541 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      601 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      661 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      721 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      781 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
      841 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1741 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     1801 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     1861 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     1921 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     1981 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2041 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     2101 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2161 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2221 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2281 gcttgagcag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     2341 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     2401 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     2461 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     2521 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     2581 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     2641 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     2701 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     2761 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     2821 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     2881 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     2941 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3001 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3061 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3121 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3181 atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa
     3241 aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata
     3301 aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatctcgg
     3361 tccgaaaacc atgcatcatc atcatcatca cagcagcggc agagaaaact tgtatttcca
     3421 gggcgcaagc cagttggatg aatttcagga atgcttgtcc aagtttactc gatataattc
     3481 agtacgacct ttagccacat tgtcatatgc tagtgatctc tataatggtt ccagtatagt
     3541 ctctagtatt gaatttgacc gggattgtga ctattttgcg attgctggag ttacaaagaa
     3601 gattaaagtc tatgaatatg acactgtcat ccaggatgca gtggatattc attaccctga
     3661 gaatgaaatg acctgcaatt cgaaaatcag ctgtatcagt tggagtagtt accataagaa
     3721 cctgttagct agcagtgatt atgaaggcac tgttatttta tgggatggat tcacaggaca
     3781 gaggtcaaag gtctatcagg agcatgagaa gaggtgttgg agtgttgact ttaatttgat
     3841 ggatcctaaa ctcttggctt caggttctga tgatgcaaaa gtgaagctgt ggtctaccaa
     3901 tctagacaac tcagtggcaa gcattgaggc aaaggctaat gtgtgctgtg ttaaattcag
     3961 cccctcttcc agataccatt tggctttcgg ctgtgcagat cactgtgtcc actactatga
     4021 tcttcgtaac actaaacagc caatcatggt attcaaagga caccgtaaag cagtctctta
     4081 tgcaaagttt gtgagtggtg aggaaattgt ctctgcctca acagacagtc agctaaaact
     4141 gtggaatgta gggaaaccat actgcctacg ttccttcaag ggtcatatca atgaaaaaaa
     4201 ctttgtaggc ctggcttcca atggagatta tatagcttgt ggaagtgaaa ataactctct
     4261 ctacctgtac tataaaggac tttctaagac tttgctaact tttaagtttg atacagtcaa
     4321 aagtgttctc gacaaagacc gaaaagaaga tgatacaaat gaatttgtta gtgctgtgtg
     4381 ctggagggca ctaccagatg gggagtccaa tgtgctgatt gctgctaaca gtcagggtac
     4441 aattaaggtg ctagaattgg tatgatgacg aagcttgtcg agaagtacta gaggatcata
     4501 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4561 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4621 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4681 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     4741 ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
     4801 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     4861 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     4921 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
     4981 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
     5041 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
     5101 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5161 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5221 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5281 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5341 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5401 cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5461 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5521 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5581 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5641 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5701 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5761 ttgctcaccc a
//
