LOCUS       sequence-426198-        6524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human COPB2 fragment M1 to A597. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_COPB2:1-597
FEATURES             Location/Qualifiers
     source          1..6524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(623..628,629..645,646..651))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1003..1094
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1019..1036
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1153..1170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1183..1203
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3132..3266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3169..3188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3223..3242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3295..3460
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3644..4098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3731..3750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3940..3961
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4124..4228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4229..4297,4298..5089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4447..4466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5260..5848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5749..5768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6002..6019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6153..6377
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(6444..6524,1..453))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
       61 cttgagcaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
      121 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
      181 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
      241 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
      301 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
      361 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
      421 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      481 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      541 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      601 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
      661 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga
      721 ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc
      781 attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca
      841 ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt
      901 ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta
      961 tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga gataattaaa
     1021 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1081 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatctcggt
     1141 ccgaaaacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
     1201 ggcatgcctc tgcgacttga tatcaaaaga aagctaactg ctagatctga tcgagttaag
     1261 agtgtggatc tgcatcctac agagccatgg atgttggcaa gtctttacaa tggcagtgtg
     1321 tgtgtttgga atcatgaaac acagacactg gtgaagacat ttgaagtatg tgatcttcct
     1381 gttcgagctg caaagtttgt tgcaaggaag aattgggttg tgacaggagc ggatgacatg
     1441 cagattagag tgttcaatta caatactctg gagagagttc atatgtttga agcacactca
     1501 gactacattc gctgtattgc tgttcatcca acccagcctt tcattctaac tagcagtgat
     1561 gacatgctta ttaagctctg ggactgggat aaaaaatggt cttgctcaca agtgtttgaa
     1621 ggacacaccc attatgttat gcagattgtg atcaacccca aagataacaa tcagtttgcc
     1681 agtgcctctt tggacaggac tatcaaggtg tggcagttgg gctcttcgtc accaaacttc
     1741 actttggaag gacatgagaa aggcgtgaat tgcattgatt actacagtgg tggggacaag
     1801 ccatacctca tttcaggtgc agatgaccgt cttgttaaaa tatgggatta tcagaataaa
     1861 acatgtgtgc agacactgga aggacatgcc caaaatgtgt cttgtgccag ctttcatcct
     1921 gagttgccaa tcattatcac aggttcagaa gatggaacag tacgtatttg gcattcaagc
     1981 acctaccggc ttgagagcac actgaattat ggaatggaga gggtatggtg cgtggccagt
     2041 ctaagagggt caaacaatgt cgctttgggc tatgatgaag ggagcatcat tgttaagctt
     2101 ggtcgggagg aacctgccat gtccatggat gccaatggaa agataatttg ggccaagcat
     2161 tcagaagtcc agcaggccaa cctaaaagca atgggagatg ctgaaattaa agatggtgaa
     2221 agattgccac tggcagtaaa ggatatgggc agttgtgaaa tataccctca gactattcag
     2281 cacaatccta atgggcggtt tgtggtggtg tgtggtgatg gggagtatat catctacaca
     2341 gcaatggcat tgagaaacaa gagctttgga tctgctcagg agtttgcatg ggcccacgat
     2401 tcttcagagt atgcaataag agagagcaac agcattgtaa agatatttaa gaactttaag
     2461 gaaaaaaaat catttaaacc agattttgga gcagaaagta tctacggcgg cttcttattg
     2521 ggagtcagat ctgtaaatgg cttagccttc tatgactggg acaatacaga actcatacga
     2581 agaattgaaa ttcagcccaa acatattttc tggtctgact ctggagagct agtctgtatt
     2641 gctactgagg aatcattttt tatccttaag tatctgtcag aaaaagtctt ggctgcacag
     2701 gaaacacatg agggagttac tgaagatggc attgaagatg cctttgaggt tcttggtgag
     2761 attcaggaaa ttgtgaaaac agggctttgg gtaggcgatt gcttcattta cacaagttct
     2821 gtgaacagat taaattatta tgttggagga gaaatagtca ccattgccca cttggacagg
     2881 acgatgtatc tcctaggcta cattcctaaa gacaacaggc tttatctggg ggataaagaa
     2941 ttgaacatca ttagctattc cctgctggtt tcagtcctgg aataccagac agcttgatga
     3001 cgaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     3061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3241 tcaatgtatc ttatcatgtc tggatctgat cactgatatc gcctaggaga tccgaaccag
     3301 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     3361 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     3421 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     3481 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     3541 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     3601 gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3661 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3721 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3781 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3841 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3901 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     3961 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4021 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4081 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4441 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4741 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5401 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5941 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6001 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     6061 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     6121 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     6181 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     6241 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     6301 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     6361 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     6421 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     6481 acttcttccc gtatgcccaa ctttgtatag agagccactg cggg
//
