LOCUS       sequence-426232-       10853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. No Cas9 control
            expression cassette. LT1 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: LT1
FEATURES             Location/Qualifiers
     source          1..10853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..86)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(320..339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1042..1066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1119..1141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_RNA        1815..1890
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3452..3454,3455..3457,3458..4168)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3497..3518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4105..4126
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5353..6063
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5498..5516)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5766..5785
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    6307..6354
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    6466..6490
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6570..7201
                     /label=oriV
                     /note="incP origin of replication"
     CDS             8612..9406
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             9705..10853
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact gagatcctct
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg gttggcatgc
      961 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     1021 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     1081 aaactgaagg cgggaaacga caatctgcta gtggatctcc cagtcacgac gttgtaaaac
     1141 gggcgccccg cggaaagctt gctagccaat tggggcccaa cgttctcgag tttttctaga
     1201 aggccttgga tccacccggg agaattcgtc gactttgcgg ccgcatcgat actgcaggag
     1261 ctcggtacct tttactagtg atatcgatca tgagcgacct gtattcagtc gactggtacc
     1321 aacatcagct ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca
     1381 ttttcttgaa ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat
     1441 cttgtttcat agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga
     1501 ttgaataaaa catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat
     1561 ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg
     1621 cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc
     1681 tgagtttata tacagctaga gtcgaagtag tgattggaac aaagcaccag tggtctagtg
     1741 gtagaatagt accctgccac ggtacagacc cgggttcgat tcccggctgg tgcagtcccc
     1801 tccaccccac agtggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1861 caacttgaaa aagtggcacc gagtcggtgc tttttttttt tttctgcttt ctgcccttgc
     1921 caatccatat ctgaagaaga aaaaaaaaca caaaattacg atttagatcc gatataacaa
     1981 aatttgaatc gcacagatcg atctctttgg agattctata cctagaaaat ggagacgatt
     2041 ttcaaatctc tgtaaaaatt ctggtttctt cttgacggaa gaagacgacg actccaatat
     2101 ttcggttagt actgaaccgg aaagtttgac tggtgcaacc aatttaatgt accgtacgta
     2161 acgcaccaat cggattttgt attcaatggg ccttatctgt gagcccatta attgatgtga
     2221 cggcctaaac taaatccgaa cggtttattt cagcgatccg cgacggtttg tattcagcca
     2281 atagcaatca attatgtagc agtggtgatc ctcgtcaaac cagtaaagct agatctggac
     2341 cgttgaattg gtgcaagaaa gcacatgttg tgatattttt acccgtacga ttagaaaact
     2401 tgagaaacac attgataatc gataaaaacc gtccgatcat ataaatccgc tttaccatcg
     2461 ttgcctataa attaatatca atagccgtac acgcgtgaag actgacaata ttatcttttt
     2521 cgaattcgga gctcaagttt gaaattcgga gaagctagag agttttctga ggtacgattc
     2581 ttcgatcctc tttgattttc ctggaaatat tttttcggtg atcgtgaaac tactggaatc
     2641 gctcgatagg tggtacgaaa ttaggcgaga ttagtttcta ttcttggcca ttatcttgtt
     2701 tcttcgccga atgatcttcc ggataaagat tttaggttag agatgaatcg tatagctaga
     2761 tttcatcacc agatagtttc tttgtctaga atctctgaaa ttctcgatag ttttcacatg
     2821 tgtaaataga ttgttcttat tcggcgattg ttgattaggg ttttgatttt cttgattatg
     2881 cgattgcaat tagggatttt ctttggtttt gtgttgatct tacgatacat tccggcaatt
     2941 gaatacgtat ggatctaaat cttgttaatt tgttgaacag gacgatggtc ccctccaccc
     3001 cacagtgggg gcaccaagag ccgagaagaa gcccgccgag aaaaaaaccg ccgctgagag
     3061 gccggtggaa gagaacaagg ctgctgaaaa ggctccggcg gagaagaaac ccaaggcggg
     3121 gaagaaattg ccaccaaagg aagccggtga caagaagaag aagcgatcta agaaaaacgt
     3181 cgaaacctac aagatctaca tcttcaaggt gctgaagcag gttcatccag atatcggaat
     3241 ttcaagcaaa gccatgggga ttatgaacag tttcatcaac gacatattcg agaagctagc
     3301 tcaggaatct tcgaaactcg ctaggtacaa caagaagccg accattacat ctcgcgagat
     3361 ccagacggct gtgagactcg tcctccccgg tgaactcgct aaacacgccg tctctgaagg
     3421 taccaaggcg gttaccaagt ttactagctc catggtgagc aagggcgagg agctgttcac
     3481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3541 ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     3601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc
     3661 ctgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3721 cgaaggctac gtccaggagc gcaccatctc tttcaaggac gacggtacct acaagacccg
     3781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
     3901 cgtctatatc acggccgaca agcagaagaa cggcatcaag gctaacttca agatccgcca
     3961 caacgttgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc catcagtccg ccctgagcaa
     4081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4141 tacacatggc atggacgagc tgtacaagta atcaggtgga tcctagataa cctttacctt
     4201 cattccctgt aaattggtac cctgcatctc gtctctgtta tgcttaagaa gttcaatgtt
     4261 tcgtttcatg taaaactttg gtggtttgtg ttttggggcc ttgtataatc cctgatgaat
     4321 aagtgttcta ctatgtttcc gttcctgtta tctctttctt tctaatgaca agtcgaactt
     4381 cttctttatc atcgcttcgt ttttattatc tgtgcttctt ttgtttaata cgcctgcaaa
     4441 gtgactcgac tctgtttagt gcagttctgc gaaacttgta aatagtccaa ttgttggcct
     4501 ctagtaatag atgtagcgaa agtgttgagc tgttgggttc taaggatggc ttgaacatgt
     4561 taatctttta ggttctgagt atgatgaaca ttcgttgttg ctaagaaatg cctgtaatgt
     4621 cccacaaatg tagaaaatgg ttcgtacctt tgtccaagca ttgatatgtc tgatgagagg
     4681 aaactgcaag atactgagct tggtttaacg aaggagaggc agtttcttcc ttccaaagca
     4741 tttcatttga caatgccttg atcatcttaa gtagagtttc tgttgtggaa agtttgaaac
     4801 tttgaagaaa cgactctcaa gtaaattgat gatcacaagt gaaagtgtat gttactaact
     4861 attgaaacca aattaacata gggtttttat ttaaataaaa gtttaacctt ctttttaaaa
     4921 aattgttcat agtgtcatgt cagaacaaga gctacaaatc acacatagca tgcataagcg
     4981 gagctatgat gagtggtatt gttttgttcg tcacttgtca ctcttttcca acacataatc
     5041 ccgacaacaa cgtaagagca tctctctctc tccacacaca ctcatgcatg catgcattct
     5101 tacacgtgat tgccatgcaa atctcctttc tcacctataa atacaaacca acccttcact
     5161 acactcttca ctcaaaccaa aacaagaaaa catacacaaa tagcaaaaca agggcgaatt
     5221 ctcaacacaa catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg
     5281 cagcaattta aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta
     5341 cgaacgatag ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5401 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5461 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5521 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5581 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5641 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5701 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5761 gacggccccg taatgcagaa gaagacgatg ggctgggagg cctcctccga gcggatgtac
     5821 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5881 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5941 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6001 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6061 tagcttggct gcaggtcgac ctgcaggcat gcccgctgaa atcaccagtc tctctctaca
     6121 aatctatctc tctctataat aatgtgtgag tagttcccag ataagggaat tagggttctt
     6181 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     6241 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagggg taccgtatga
     6301 tctagggaag ttcctattcc gaagttccta ttctctgaaa agtataggaa cttctttgcg
     6361 tattgggcgc tcttggcctt tttggccacc ggtcgtacgg ttaaaaccac cccagtacat
     6421 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     6481 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     6541 cgatacaggc agcccatcag tccactagag acgctcaccg ggctggttgc cctcgccgct
     6601 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     6661 agacaccgca gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     6721 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     6781 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     6841 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     6901 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     6961 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     7021 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     7081 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     7141 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     7201 tctcgcgttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt cgatactatg
     7261 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaagttttag
     7321 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagggaaggt gcgaataagc
     7381 ggggaaattc ttctcggctg actcagtcat ttcatttctt catgtttgag ccgatttttt
     7441 ctcccgtaaa tgccttgaat cagcctattt agaccgtttc ttcgccattt aaggcgttat
     7501 ccccagtttt tagtgagatc tctcccactg acgtatcatt tggtccgccc gaaacaggtt
     7561 ggccagcgtg aataacatcg ccagttggtt atcgtttttc agcaacccct tgtatctggc
     7621 tttcacgaag ccgaactgtc gcttgatgat gcgaaatggg tgctccaccc tggcccggat
     7681 gctggctttc atgtattcga tgttgatggc cgttttgttc ttgcgtggat gctgtttcaa
     7741 ggttcttacc ttgccggggc gctcggcgat cagccagtcc acatccacct cggccagctc
     7801 ctcgcgctgt ggcgcccctt ggtagccggc atcggctgag acaaattgct cctctccatg
     7861 cagcagatta cccagctgat tgaggtcatg ctcgttggcc gcggtggtga ccaggctgtg
     7921 ggtcaggcca ctcttggcat cgacaccaat gtgggccttc atgccaaagt gccactgatt
     7981 gcctttcttg gtctgatgca tctccggatc gcgttgctgc tctttgttct tggtcgagct
     8041 gggtgcctca atgatggtgg catcgaccaa ggtgccttga gtcatcatga cgcctgcttc
     8101 ggccagccag cgattgatgg tcttgaacaa ttggcgggcc agttgatgct gctccagcag
     8161 gtggcggaaa ttcatgatgg tggtgcggtc cggcaaggcg ctatccaggg ataaccgggc
     8221 aaacagacgc atggaggcga tttcgtacag agcatcttcc atcgcgccat cgctcaggtt
     8281 gtaccaatgc tgcatgcagt gaatgcgtag catggtttcc agcggataag gtcgccggcc
     8341 attaccagcc ttggggtaaa acggctcgat gacttccacc atgttttgcc atggcagaat
     8401 ctgctccatg cgggacaaga aaatctcttt tctggtctga cggcgcttac tgctgaattc
     8461 actgtcggcg aaggtaagtt gatgactcat gatgaaccct gttctatggc tccagatgac
     8521 aaacatgatc tcatatcagg gacttgttcg caccttcctt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcaccttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacacc ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatattggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc
     9421 gcaacgatgc tggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct
     9481 ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca
     9541 agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg
     9601 ccgataaggt ggattatctg gacaccaagg caccaggcgg atcaaatcag gaataagggc
     9661 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg
     9721 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa
     9781 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga
     9841 tggcccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc
     9901 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt
     9961 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg
    10021 ccggcgagga cctggcaaaa caggtcagcg aggccaagca agccgcgttg ctgaaacaca
    10081 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca
    10141 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga
    10201 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga
    10261 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt
    10321 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt
    10381 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt
    10441 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg
    10501 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg
    10561 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat
    10621 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc
    10681 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca
    10741 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg
    10801 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tag
//
