LOCUS       sequence-426238-        5867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human WDR73. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR73:1-378
FEATURES             Location/Qualifiers
     source          1..5867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1157..1291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1194..1213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1248..1267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1320..1485
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1669..2123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1756..1775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1965..1986
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2149..2253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2254..2322,2323..3114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2472..2491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3285..3873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3774..3793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4027..4044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4178..4402
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4469..5002)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5172..5177,5178..5194,5195..5200))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5552..5643
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5568..5585
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5702..5719
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5732..5752
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttgttgctgg ctatgaaagc ctgaaaaaga atgaaattct tcatctgaaa ttacctctca
       61 gactttctgt aaaggaaaac aagggcttat tcccagaaag agatttcaaa gtgcgccatg
      121 gaggattttc agacaggtct atctttgatc taaagcatgt gccacatacc agattgctgg
      181 ttaccagtgg ccttccaggt tgttatctgc aggtgtggca ggttgcagag gacagtgatg
      241 tcattaaagc tgtcagcacc attgctgtgc atgagaaaga ggagagtctc tggcctaggg
      301 tggccgtctt ctccacattg gcacccggag tcctccatgg ggcgaggctc cgcagtctgc
      361 aggtcgttga tctggagtcc cggaagacca cgtacacctc agatgtcagt gacagtgagg
      421 agctgagtag cctgcaggtc ctagatgcag acacctttgc cttctgctgt gcttcgggcc
      481 ggctggggct tgttgacacc cggcagaagt gggcaccgtt ggagaatcgc agccctggcc
      541 ctgggtctgg tggagagaga tggtgtgctg aagttgggag ctggggccag ggccctgggc
      601 ccagcattgc cagccttggc tcagatgggc gtctttgtct tcttgacccc cgggatctct
      661 gccatcctgt gagctcagtc cagtgcccag tatccgtacc tagccctgac ccagagctgc
      721 tgcgagtgac ttgggcccca ggcctgaaga attgcttggc catctcaggt tttgatggta
      781 cagtccaggt ctatgatgcc acatcttggg atggaacacg gagccaagat ggaacacgga
      841 gccaagtaga acctctcttc actcacagag gtcacatctt cctagatgga aatgggatgg
      901 accctgctcc tttggtcacc acccacacct ggcatccctg cagaccaagg actttgttat
      961 cagcaacaaa tgatgcctct ctgcatgtgt gggactgggt ggacctttgt gccccccgct
     1021 gatgacgaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     1081 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1141 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1261 actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta ggagatccga
     1321 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     1381 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     1441 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct
     1501 tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg
     1561 ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg
     1621 taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac gcgccctgta
     1681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1921 agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac tcttgttcca
     1981 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2041 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2101 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     2161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4021 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4081 acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc aaaatcggtt
     4141 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     4201 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     4261 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     4321 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     4381 gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac tccgcggccg
     4441 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
     4501 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
     4561 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgagcagat
     4621 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga
     4681 tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa
     4741 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     4801 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     4861 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     4921 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     4981 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     5041 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     5101 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     5161 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     5221 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt
     5281 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct
     5341 tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa
     5401 gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc
     5461 tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg
     5521 gttggctacg tatactccgg aatattaata gatcatggag ataattaaaa tgataaccat
     5581 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
     5641 aatattccgg attattcata ccgtcccacc atcgggcgcg gatctcggtc cgaaaaccat
     5701 gcatcatcat catcatcaca gcagcggcag agaaaacttg tatttccagg gcatggatcc
     5761 tggggacgac tggctggtgg aatccttgcg cttgtaccag gatttctatg cattcgacct
     5821 gtcaggagcc actcgagtcc ttgaatggat tgatgacaaa ggagtct
//
