LOCUS       sequence-426255-       12615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-tagged mouse Mili in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Arif A, Bailey S, Izumi N, Anzelon TA, Ozata DM, Andersson C, 
            Gainetdinov I, MacRae IJ, Tomari Y, Zamore PD
  TITLE     GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute 
            proteins.
  JOURNAL   Nature. 2022 Aug;608(7923):618-625. doi: 10.1038/s41586-022-05009-0.
            Epub 2022 Jun 30.
  PUBMED    35772669
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05009-0"; journalName: "Nature"; date: 
            "2022-08"; volume: "608"; issue: "7923"; pages: "618-625"
COMMENT     Sequence Label: pSCALPS-Flag-Mili-EGFP
FEATURES             Location/Qualifiers
     source          1..12615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..248
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     422..441
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        516..620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(621..689,690..1481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(839..858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1652..2240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2141..2160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             2311..2491
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3009..3597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3115..3126
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3524..3544
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4707..5420,5421..5423,5424..5426))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4752..4773)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5101..5120
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5360..5381
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5438..5990
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5600..5619)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5829..5846
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(7228..7239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8947..8967)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(8986..9531)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             complement(9544..9609)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(9616..9633)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(9651..10058)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    10195..10312
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10610..10654)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10838..11071
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11568..11693
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11740..11920
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(11938..12136)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11963..11983)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        12138..12517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(join(12529..12615,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12543..12615,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(join(12597..12615,1))
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agcgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
      301 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
      361 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
      421 gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga tctcccgatc
      481 cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      541 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      601 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      661 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      721 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      781 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      841 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      901 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      961 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1021 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1081 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1141 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1201 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1261 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1321 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1381 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1441 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1501 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1561 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1621 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1681 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1741 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1801 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1861 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1921 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1981 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2041 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2101 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2161 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2221 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2281 gctggccttt tgctcacatg aaacgggccc tgctagagat tttccacact gactaaaagg
     2341 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     2401 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     2461 aggctcagat ctggtctaac cagagagacc cagtacagtc cggatgcagc tctcgggcca
     2521 tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa tacttctctc tccgggtcat
     2581 ccatcccatg caggctcaca gggtgtaaca agcgggtgtt ctctccttca ttggcttctt
     2641 ctaccttctc ttgctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
     2701 tgatccctgg ccctggtgtg tagttctgcc aatcagggaa gtagccttgt gtgtggtaga
     2761 tccacagatc aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct
     2821 tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta
     2881 cctgaggtgt gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat
     2941 cagcattggt agctgctgta ttgctacttg tgattgctcc atgtttttct aggtctcgac
     3001 gcgattaagc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     3061 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     3121 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     3181 cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag
     3241 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     3301 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     3361 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     3421 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     3481 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     3541 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattagc
     3601 gatcgctcag gcgccgggtt tgcgtgtcat gcaccaggtc ctaggtcctt cggggacctc
     3661 cacatctgcg gtcacagtaa agcccagccg ttcatagaag ggcaggtttc gtggagcaga
     3721 ggtctccaga aatgcaggga ctccagctcg ttctgcagcc tccactcctg gcaggaccac
     3781 agcacttccc agtcctttgc cctgatggtc aggagagacc cccacggtag ccaggaacca
     3841 tgctggttcc ttaggcctgt gtggggccag cagtccctcc atctgctgct gtgctgccag
     3901 cctggagccg ctcagttctg ccattctagg gccgatctct gcaaagacag ctccggcttc
     3961 cacgctctct ggtgtggtcc agacagccac agctgctcca tcgtctgcga cccagacctt
     4021 cccaatgtcc aggcccactc gggtcaggaa cagttcctgc agctcagtca ctctctcgat
     4081 atgccgatct gggtccacag tgtggcgtgt ggcagggtaa tcggcaaaag cggcggccag
     4141 tgttctcaca gcccttggga catcatctct tgtagccagc ctcacggtag gtttgtattc
     4201 ggtcatggcg gcacgcgtgg ctaatagtac acggttttcc tcggcggtgg cgtctgcaaa
     4261 acggcacgag cctggcctcc cgcccctttt ataccacgtt cggccccgcc cgcaacccgg
     4321 tctcgcgcct ccgtcccggc caatcgggtc tgcgacttcg ccagagtgac agtgcatctg
     4381 gcctatagac ggaagccggc gctgatgggg acgacacatg aggcgcgcgt gcgcgctaac
     4441 gcgcggaggc aggttcccgc ctgcgggctc cgtcgcggtg gctgcggcat gggactcggg
     4501 gcggggcggc agtacgggac gcggggcacg ggggagggtc agtgcggaac gggggtgggc
     4561 agcatggcgc gcgcacgcgg gagacggaag cgcggcggct cttcggccgt tgtcaagcga
     4621 tgaggcgcgt ctccccattg cacagagggg gcaactgagg cgaacgcagg cctcaaggtc
     4681 gcgccgccag tcaaggtcga gaattcttac ttgtacagct cgtccatgcc gagagtgatc
     4741 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     4801 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     4861 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
     4921 cggatcttga agttcgcctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
     4981 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     5041 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     5101 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     5161 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
     5221 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     5281 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccagacacg
     5341 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     5401 aacagctcct cgcccttgct caccatggtt gtggccatat tatcatcgtg tttttcaaag
     5461 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     5521 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     5581 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     5641 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     5701 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     5761 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     5821 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     5881 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
     5941 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt gttaacccgc
     6001 ggcttacagg aagaacaggt tcccacacag ctggatggct ggctcatgat gcaaaatctg
     6061 tccggacagg aaagctagct tgtgggcata cttgcaagga gctggaactc ggatggtacc
     6121 aggccaattc cagtacatgt ggcatagttt gaaagtcaac ctctgcatgt gatcagggct
     6181 cagatttgca gtgttcagaa cacagatgta gtgtgtaggt atgccacagc cctgtcgcac
     6241 atgatgggca agaaggtaga aatccaccca ctcacagctg gttatggtat gatcaaccac
     6301 agtcccgggg gagggggtta cgaagtgatc aggagcagca aggtacagat tggtgctgat
     6361 tttcttctga actacaaaca ccaccatctt ggggtggtag ttatcaaagg cttcaaaaca
     6421 cttctgcagc tgagggatct cgtagttggc aactgtcttt agctggccat cagacactcc
     6481 atctcggtac accacaattt tctctgggag acaatggttc acctcataat actttttcaa
     6541 ggaacccacc aggcagagct tcaggctgtc cacaatctcc tgatgtggca tctggaacac
     6601 caccctcgag taccatttgg tgagtgtgag atttatgctg gccacgaagc cgaccacaga
     6661 gcgcatgcct ctgctggggt catggtacac atccattcca atcaccatta gttgtttcag
     6721 cggaatatcc actccccaga gctcaccacc cagtttacag ttcatctgaa gtaaaatttt
     6781 ctgagccacg ctccgaagcc tggtgggctg accaatggtt cggacattga tgacctgtga
     6841 gggcactggg gactgcacgc agcacagctt cttgatggct ccatagagat catcacgtgt
     6901 gcccatgatg atgcaaacga ccatttgtat cttcccctca actcccagta aggactgaat
     6961 ggtcctgata taggtctcta ttcggtcatc cttcagctca acccaggctg gggggcttat
     7021 gcgcatgcca atgggcccgg caatcttttc caacatgtta accagttctc tggcttggtc
     7081 cattgctctc tttggataaa agagtgccca gaaatgcatg ggaatagtta gaatggaagc
     7141 atctctggtc acttccttaa cccagttcag gtcctccgat gtgacaaatg aagtgttcct
     7201 taagttgatc ctctccattg gcagaagccg accttcaatc ttgtggacat ctttatgcag
     7261 actgagcccc cagcgggtca gctcattgct ggctgtctcg ttttgtgaaa ttctctgcag
     7321 caggcattcc aaagcaccgt ggtgctgctt ggggctcagg ttaatctgct gagtcaagtc
     7381 cttcatggcc ctgaagtcct tcttcatctt ctcagggatc cccgtcatga aggagagctc
     7441 gggcagcagc aggatctcgc ccttcagcaa catgccatgg ttattctgtc tctcactggg
     7501 ccggtggatc agcagcggct ggtcatcttc cttgactgtg atcccatagt ttttgctgta
     7561 gtattccagg aatgtgattt ccttcccgtc cgacatgaca aagctgtctt taggggtctt
     7621 gttccagtcc acatcatcga ttcggtaggt acgattgttg tagcgcgtga tgacaatgct
     7681 gccaaccaga agcttgctgc actcgtcctg gaagtgctcc ttgttctgct ggtagatagc
     7741 atgcatgaca tccagcacag agtcgttccg aatgacctta tgagagacat cagcgagcag
     7801 gaagagaccc ccgtctgtcc tccggatact agccgcatag ccaggccaga tctgcaatct
     7861 gtgttgctgc agtaccatgg cacttgtagg gtcatagaag tttctcccca caagcttcat
     7921 atccagaagt ttcattaccc gccggaagac aacattgtag aaggggatgc acaggtcaga
     7981 acacggctcc aggatctttg tcagctgaat cttgatactg atctcggcat cgtcagtttt
     8041 cctctgactt tttaactcaa caacttgttg aagcttaaca ggaagataaa gaatagagcc
     8101 atcaaaagca gtgacgtttc cagtgacaga ctggtggtcc ttcaacatgc caaacctcat
     8161 gcttttgcat tccacactgg ggctgaaagt cacatggtac tgataaactg cttcgttatg
     8221 acactggatt ttgatgaggt tcagtcccaa agactgagga gttccttttg atccttgctt
     8281 gaccaaaagc tctttttcct ttcgttccat agtcaggacc ggagggggat cggcagagtg
     8341 cagtgaagtt ggagacagag ctggaggctg tggcagccgt gggggatccc gagctgtgag
     8401 gccaaaggca ctgggaggtt tgtccattcc tctgcctata ctactggctg ctcgtcccag
     8461 tggtaacaga gagacctcag aactacctct tcccagcatt ctactccaac caacagaagc
     8521 ctctgccagt ttgctgtccc cagctgccag caccgaagga tcaggcaaag aggaacgggg
     8581 ttcttctctg tccttgcgga ccatgttagc agacaagcct cgacctagaa ctcctctgcc
     8641 taaaggaggc acttctcgtt ttgaaggagg ccggaatgct gtgtcaagac ccatgccacg
     8701 gaacatggac accaaaccta cagaatcctt ttgtactggc tggagtggtg ggctggagtc
     8761 ttctggtttt ctaaacacaa ggcctctgcc tgcaggtcct gccctacccc aagctggttc
     8821 caaaggtcta ggagcttgag gccaacagcc tggcatccgc acacactgag atgggtggac
     8881 tggggtgggc cccctgaaca acggcctgac aggatccatt ccagcatcgc cagagccgcc
     8941 agagcctccc tggaaataca ggttttcgcc agagccgcca gagccaccca gcccaggctt
     9001 gcccagtctg tggccctcgt gggccagcag ccactctttc acggcgagcc cgccctcgta
     9061 gccccccacg tccaggtcgc cctgcaccac ccggtggcag gggatcagaa tgggcacggg
     9121 atttccgctc agggcggttt tcacggcggc ggtggcggcg ggattgccgg ccagggcggc
     9181 caggtggctg tagctgatga cctctccgaa cttcaccact ttcagcagtt tccacagcac
     9241 ctggcgggta aagctctcct gctggaacac tgggtggtgc agggctggca cagggaactc
     9301 ctcgatggcc tcaggctggt gaaagtaggc gttgagccag gcggtggcct gcatcagtgg
     9361 ctctggtccg cccagcacgg cggctggggc aggcacttcc acggcgtcgg cggcagatgt
     9421 tcctttgccc aggaagatga tacggtgcag gccctgttcg cacccagaca gttccagctt
     9481 gcccagaggg ctatccaggg tggtgcgctt catttcgcag tctttgtcca tgccgccaga
     9541 gcccttgtcg tcgtcgtcct tgtagtcgat gtcgtggtcc ttgtagtcac cgtcgtggtc
     9601 cttgtagtcg ccaccatgat ggtggtgatg gtgcatgatg cttctagacc cccgggcgac
     9661 tcagtctgtc ggaggactgg cgcgccgagt gaggggttgt gagctctttt atagagctcg
     9721 ggaagcagaa gcgcgcgaac agaagcgaga agcaggctga ttggttaatt caaataaggc
     9781 gcagggtcat ttcaggtcct tgggggagcc tggaaacatc tgatgggtct taagaaactg
     9841 ctgagggttg ggccatatct ggggaccatc tgttcttggc ctcgggccgg ggccgaaact
     9901 gcggtgacca tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg cagatatcct
     9961 gtttggccca acgttagcta ttttcatgta cccgcccttg atctgaactt ctctattctt
    10021 ggtttggtat ttttccatgc cttgcaaaat ggcgttactg cagctagctt gccaaaccta
    10081 caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt ttattttatc
    10141 ggggctgcag ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
    10201 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
    10261 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
    10321 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
    10381 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
    10441 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
    10501 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
    10561 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
    10621 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
    10681 ttaaggagtg tattaagctt gtgtaattgt taattctctg tcccactcca tccaggtcgt
    10741 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
    10801 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
    10861 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
    10921 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
    10981 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
    11041 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
    11101 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
    11161 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
    11221 cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
    11281 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
    11341 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
    11401 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
    11461 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
    11521 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
    11581 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
    11641 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
    11701 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
    11761 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
    11821 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
    11881 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
    11941 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
    12001 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
    12061 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
    12121 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
    12181 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
    12241 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
    12301 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
    12361 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
    12421 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
    12481 attgattact attaataact agtcaataat caatgtcaac gcgagctttt tgcaaaagcc
    12541 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
    12601 ggcctctgca taaat
//
