LOCUS       sequence-426366-        9220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9220)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor
FEATURES             Location/Qualifiers
     source          1..9220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..425
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    199..217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    271..289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     334..358
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      432..441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(438..440,441..443,444..1154)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(483..504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(744..763)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1091..1112
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1200..1220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1233..1277
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     intron          2842..2907
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(3353..3370)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3359..3583
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3600..3721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3611..3630)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3665..3684
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3752..4351)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3835..3855)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4332..4351
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4358..4411)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4417..4442
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    4449..5252
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(5272..5860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5352..5371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6031..6822,6823..6891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6654..6673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6892..6996)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7033..7869
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    7877..7932
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(7961..8707)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             8730..8998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(join(9011..9220,1..2))
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ccactgacgg gcaccggaga gatcttttcg tcttcaacaa ttcttcgagt ttactcccta
       61 tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga
      121 ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag
      181 aacgtatatc cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta
      241 tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa cgtataagct
      301 ttaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
      361 ctggagcaat tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc
      421 gtaaagtcga cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      481 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      541 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      601 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      661 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      721 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      781 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      841 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      901 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      961 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1021 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     1081 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1141 acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc cgctctagag
     1201 agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac
     1261 gccagcacat ggacagcgga ggtggaggtc tcgagatggc agaccacaac cctgacagcg
     1321 attctacacc tcgtacactg ctgcgtcgtg tgctggatac tgctgatcct cgtactcctc
     1381 gtcgtcctcg tagcgctcgt gctggtgctc gccgtgccct gctggaaaca gcctctcctc
     1441 gtaaactgag tggccagacc cgtactattg cccgtgggcg ttcacatgga gcccgctcgg
     1501 tcggtcgtag tgctcacatt caagcctcgg gacacctgga agaacaaacc cctcgtaccc
     1561 tgctgaaaaa catcctgctg actgcccctg aaagctctat tctgatgccg gaaagcgtcg
     1621 ttaaaccggt tcctgctcct caagccgttc aaccttctcg tcaagagtcc tcctgtggat
     1681 ctctggagct gcaactgcct gaactggagc ctcctacaac actggcaccg gggctgctgg
     1741 cgcctggtcg tcgtaaacaa cgtctgcgtc tgagcgtgtt tcaacaaggt gttgatcagg
     1801 gcctgagtct gtctcaagaa cctcaaggca acgctgatgc ttctagtctg acccgttctc
     1861 tgaacctgac ctttgctact ccactgcaac ctcaaagcgt ccaacgtcct ggactggctc
     1921 gccgccctcc tgctcgccgt gccgttgatg tgggtgcttt tctgcgtgac ctgcgtgata
     1981 caagcctggc acctccgaat attgtgctgg aggatacaca ggaattcccg cggggaagtg
     2041 gaagtggatc cggaggcagc ggtggctccg ggggctctgg aggcagcatg aagatggagg
     2101 agctgttcaa gaagcacaag atcgtggccg tgctgagagc caacagcgtg gaggaggcca
     2161 agaagaaggc cctggccgtg ttcctgggcg gcgtgcacct gatcgagatc accttcaccg
     2221 tgcccgacgc cgacaccgtg atcaaggagc tgagcttcct gaaggagatg ggcgccatca
     2281 tcggcgccgg caccgtgacc agcgtggagc agtgcagaaa ggccgtggag agcggcgccg
     2341 agttcatcgt gagcccccac ctggacgagg agatcagcca gttctgcaag gagaagggcg
     2401 tgttctacat gcccggcgtg atgaccccca ccgagctggt gaaggccatg aagctgggcc
     2461 acaccatcct gaagctgttc cccggcgagg tggtgggccc ccagttcgtg aaggccatga
     2521 agggcccctt ccccaacgtg aagttcgtgc ccaccggcgg cgtgaacctg gacaacgtgt
     2581 gcgagtggtt caaggccggc gtgctggccg tgggcgtggg cagcgccctg gtgaagggca
     2641 cccccgtgga ggtggccgag aaggccaagg ccttcgtgga gaagatcaga ggctgcaccg
     2701 agtgatagct cactcattag gcaccccagg ctttacactt tatgcttccg acctgcagcc
     2761 ccttggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     2821 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     2881 ctgattctaa ttgtttgtgt attttagatc acagtcccaa ggctcatttc aggcccctca
     2941 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg
     3001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattcctt
     3061 aagtacgtac cgcgggctag cggatccatg catacatgtg atatcactag tgattttagg
     3121 gagagtttag tatccctggt tcagaactcc agaaaactac agaacattag agacaatgtg
     3181 gaaaaggaat cgaaacgact gaaaatatct taacccggga tccaccggat ctagataact
     3241 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3301 cctccccctg aacctgaaac ataaaatgaa tgcaattaaa cccgctgatc agcctcgact
     3361 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3421 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3481 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3541 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggggtacca cagacatgat
     3601 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3661 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3721 tggggtgggc gaagaactcc agcatacgcg ttcaggcacc gggcttgcgg gtcatgcacc
     3781 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
     3841 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg
     3901 ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg
     3961 agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc
     4021 cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga
     4081 tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccactgcgg
     4141 cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt
     4201 cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg
     4261 ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg
     4321 cgaggcgcac cgtgggcttg tactcggtca tatcgatagg gccgggattc tcctccacgt
     4381 caccgcatgt tagaagactt cctctgccct ctccgccctg tgggaggaag agaagaggtc
     4441 aggcgcgtgc cccactgtgg ggtggagggg acagataaaa gtacccagaa ccagagccac
     4501 attaaccggc cctgggaata taaggtggtc ccagctcggg gacacaggat ccctggaggc
     4561 agcaaacatg ctgtcctgaa gtggacatag gggcccgggt tggaggaaga agactagctg
     4621 agctctcgga cccctggaag atgccatgac agggggctgg aagagctagc acagactaga
     4681 gaggtaaggg gggtagggga gctgcccaaa tgaaaggagt gagaggtgac ccgaatccac
     4741 aggagaacgg ggtgtccagg caaagaaagc aagaggatgg agaggtggct aaagccaggg
     4801 agacggggta ctttggggtt gtccagaaaa acggtgatga tgcaggccta caagaagggg
     4861 aggcgggacg caagggagac atccgtcgga gaaggccatc ctaagaaacg agagatggca
     4921 caggccccag aaggagaagg aaaagggaac ccagcgagtg aagacggcat ggggttgggt
     4981 gagggaggag agatgcccgg agaggaccca gacacgggga ggatccgctc agaggacatc
     5041 acgtggtgca gcgccgagaa ggaagtgctc cggaaagagc atccttgggc agcaacacag
     5101 cagagagcaa ggggaagagg gagtggagga agacggaacc tgaaggaggc ggcagggaag
     5161 gatctgggcc agccgtagag gtgacccagg ccacaagctg cagacagaaa gcggcacagg
     5221 cccaggggag agaatgctgg tcagagaaag caggcgcgcc ttgctggcgt ttttccatag
     5281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5701 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5821 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5881 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5941 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6001 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6061 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6121 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6181 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6241 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6301 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6361 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6421 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6481 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6541 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6601 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6661 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6721 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6781 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6841 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6901 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6961 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgcttaa
     7021 gcccttgccc ttagagcaga gccaggaacc cctgtaggga aggggcagga gagccagggg
     7081 catgagatgg tggacgagga agggggacag ggaagcctga gcgcctctcc tgggcttgcc
     7141 aaggactcaa acccagaagc ccagagcagg gccttaggga agcgggaccc tgctctgggc
     7201 ggaggaatat gtcccagata gcactgggga ctctttaagg aaagaaggat ggagaaagag
     7261 aaagggagta gaggcggcca cgacctggtg aacacctagg acgcaccatt ctcacaaagg
     7321 gagttttcca cacggacacc cccctcctca ccacagccct gccaggacgg ggctggctac
     7381 tggccttatc tcacaggtaa aactgacgca cggaggaaca atataaattg gggactagaa
     7441 aggtgaagag ccaaagttag aactcaggac caacttattc tgattttgtt tttccaaact
     7501 gcttctcctc ttgggaagtg taaggaagct gcagcaccag gatcagtgaa acgcaccaga
     7561 cagccgcgtc agagcagctc aggttctggg agagggtagc gcagggtggc cactgagaac
     7621 cgggcaggtc acgcatcccc cccttccctc ccaccccctg ccaagctctc cctcccagga
     7681 tcctctctgg ctccatcgta agcaaacctt agaggttctg gcaaggagag agatggctcc
     7741 aggaaatggg ggtgtgtcac cagataagga atctgcctaa caggaggtgg gggttagacc
     7801 caatatcagg agactaggaa ggaggaggcc taaggatggg gcttttctgt caccaatcct
     7861 gtccctagta agagcttgag agacacaaaa aattccaaca cactattgca atgaaaataa
     7921 atttccttta ttccggaata aaggaaattt agtacccggg ttaccctggg agcatgtcaa
     7981 ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat
     8041 cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc
     8101 actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt
     8161 cggctccctg ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg
     8221 tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc
     8281 ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt
     8341 gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg
     8401 tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg
     8461 cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat
     8521 gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc
     8581 agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt
     8641 cgataccgac tccattgagt aattccagag cagagtttat gactttgctc ttgtccagtc
     8701 tagacatggt gcttaattaa cacagcttgg atctggaacg gcgcagaaca gaaaacgaaa
     8761 caaagacgta gagttgagca agcagggtca ggcaaagcgt ggagagccgg ctgagtctag
     8821 gtaggctcca agggagcgcc ggacaaaggc ccggtctcga cctgagcttt aaacttacct
     8881 agacggcgga cgcagttcag gaggcacctc aggcgggagg cggcagaacg cgactcaacc
     8941 ggcgtggatg gcggcctcag gtagggcggc gggcgcgtga aggagagatg cgagcccctc
     9001 gaagcttcag ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     9061 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     9121 cactttccca gtttaccccg cgccaccttc tctaggcacc cgttcaattg ccgacccctc
     9181 cccccaactt ctcggggact gtgggcgatg tgcgctctgc
//
