LOCUS       sequence-42660-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     pHyVec Cloning Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHyVec1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(523..543)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(523..541)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(562..578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(562..578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(575..597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(610..616,617..634,635..640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    655..676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(793..810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actatacaaa tagcattcgt agaattcaca attcgattat atttatactg gactattttt
       61 acatctgttc ggttattttc acatttattt ttctatatat atcttataaa cgttttaaaa
      121 cccatgtaat ttttgttaag ctgtaatata aaagacgtcc taacaaactt cttttattac
      181 tgaatttcct ttaattataa taaataacaa gttttaaaat aaattcaggc aattaaggcg
      241 ctcctgaggt actaaaatta atgtaaacat ttaaaattaa cttggatggt cttaagtact
      301 gtactcgtga ttttgttata ctttattatt agaaaagtcg tctattaact ttttgttcct
      361 taatttactt gattaaattg tcgcttaatt tatcaaatca ggttttgcgc gttattttag
      421 agaaaaactt attagaaaaa tgaataagca aagtttaggc taacatgttt ttttattatt
      481 ttaaatagtt caagtcaatg acgtataaac cagcttttgt tccctttagt gagggttaat
      541 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcgg
//
