LOCUS       sequence-426715-        5713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV reporter genome plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes 
            C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G,
            Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen
            AT, Barry AJ, Eid FE, Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human transferrin receptor 
            mediates brain-wide gene delivery.
  JOURNAL   Science. 2024 Jun 14;384(6701):1220-1227. doi: 
            10.1126/science.adm8386. Epub 2024 May 16.
  PUBMED    38753766
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adm8386"; journalName: "Science"; date: 
            "2024-06-14- 14"; volume: "384"; issue: "6701"; pages: "1220-1227"
COMMENT     Sequence Label: pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA
FEATURES             Location/Qualifiers
     source          1..5713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        210..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..792
                     /label=chicken beta-actin promoter
     regulatory      808..817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2460..2936
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2659..2678)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2976..3116
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2976..3105
                     /label=AAV2 ITR
     rep_origin      3191..3646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3278..3297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3488..3509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3663..3682)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3782..3804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3842..3860)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3928..4032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4033..4101,4102..4893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4251..4270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5064..5652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5553..5572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgcggcccaa cggtaccgcc accatggagt tcagctcccc ctccagggag
      841 gagtgcccta aaccactgtc aagagtctct atcatggccg gatccctcac cggcctgctg
      901 ctgctgcagg ccgtgagctg ggcctctggc gctagaccat gcatccctaa gtccttcggc
      961 tactcctccg tggtgtgcgt gtgcaacgcc acctactgcg actccttcga ccctcctact
     1021 ttccccgctc tggggacctt cagccggtac gagtccaccc gaagtggcag gcgcatggag
     1081 ctgagcatgg ggcctatcca ggctaaccac acaggaacag gcctgctgct gaccctgcag
     1141 cccgaacaga agttccagaa ggtgaagggc tttggaggcg ccatgaccga cgctgctgcc
     1201 ctgaacattc tcgccctgtc cccccccgct cagaacctgc tcctgaagag ctacttcagc
     1261 gaagaaggga tcgggtacaa catcatcagg gtgcccatgg ccagctgcga cttttccatt
     1321 agaacataca cctatgctga tactcccgat gactttcagc tgcacaactt tagcctgcca
     1381 gaagaggaca caaagctgaa gatccccctg atccacaggg ccctgcagct cgctcagaga
     1441 cctgtgtccc tgctggccag cccatggaca agtcccacct ggctgaaaac caacggcgcc
     1501 gtgaacggca agggttccct gaaggggcag cccggagaca tctaccatca gacctgggca
     1561 aggtactttg tgaaattcct ggacgcctat gccgagcaca aactgcagtt ctgggctgtg
     1621 actgctgaga acgagccttc cgcaggactg ctgagcggat accctttcca gtgtctgggc
     1681 tttacccctg agcaccagcg cgacttcatc gctcgggacc tgggacccac cctggctaat
     1741 tccacccacc acaacgtgag actgctgatg ctggacgatc agagactgct gctgccacac
     1801 tgggccaagg tggtgctgac tgacccagag gccgcaaagt acgtgcacgg catcgctgtg
     1861 cactggtacc tggattttct ggctccagcc aaggctaccc tgggcgaaac acaccggctg
     1921 ttccctaaca caatgctgtt cgcctccgag gcctgcgtgg gctccaagtt ctgggagcag
     1981 agcgtgagac tgggtagctg ggacagaggc atgcagtaca gccactcaat cattacaaat
     2041 ctcctgtacc acgtggtggg gtggaccgat tggaatctgg ctctgaaccc cgagggagga
     2101 ccaaactggg tgcgcaactt cgtggatagc ccaatcatcg tggacatcac caaagatacc
     2161 ttctataagc agcctatgtt ttatcacctg ggccattttt ccaagttcat cccagagggc
     2221 tcacagcggg taggcctggt tgccagccag aagaacgacc tggacgccgt ggcactcatg
     2281 caccccgatg ggtctgccgt ggtggtggtg ctgaacagat cctctaagga cgtgcccctg
     2341 actattaagg acccagccgt ggggttcctg gagacaatct ctccaggcta cagcatccac
     2401 acctacctgt ggcggagaca gtaagaattc gatatcaaag cgctgctcga gagatctacg
     2461 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     2521 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     2581 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     2641 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2701 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2761 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2821 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     2881 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     2941 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3001 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3061 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3121 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3181 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3241 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     3301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3481 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     3541 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     3601 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3661 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3721 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3781 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3841 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3901 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4141 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4201 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4261 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4321 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4381 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4441 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4501 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4561 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4621 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4681 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4741 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4801 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4861 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4921 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4981 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5701 tttgctcaca tgt
//
