LOCUS       sequence-426729-        8613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T,
            Qi Y, Zhang Y
  TITLE     High performance TadA-8e derived cytosine and dual base editors with
            undetectable off-target effects in plants.
  JOURNAL   Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
  PUBMED    38877035
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-14"; volume: "15(1):5103. doi"; pages: " 
            10.1038/s41467-024-49473-w"
COMMENT     Sequence Label: pYPQ265E6
FEATURES             Location/Qualifiers
     source          1..8613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..105
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             145..165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             766..786
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(811..834,835..837,838..4911)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4824..4832
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4912..4959
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4996..5244
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5275..5523
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5566..5586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5608..5860
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5905..6028
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6045..6064)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6046..6064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6069..6085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6069..6085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6516..7307
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7400..7988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7889..7908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8142..8159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8318..8345)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8437..8523)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8572..8594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8586..8603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8587..8603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
       61 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccgat gaaacggaca
      121 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      181 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgaaggc
      241 gcggggcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      301 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      361 ggcctggtca tgcagaacta cagactcttt gacgccaccc tgtacgtgac attcgagcct
      421 tgcgtgatgt gcgccggcgc catgatcaac tctaggatcg gccgcgtggt gtttggcgtg
      481 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      541 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      601 gatttctatc ggatgcctag acaggtgttc aacagccaga agaaggccca gagctccatc
      661 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
      721 gcaacacctg aaagcagcgg gggcagcagc ggggggtcaa tggctccgaa gaagaagagg
      781 aaggttggca tccacggggt gccagctgct gacaagaagt actcgatcgg cctcgctatt
      841 gggactaact ctgttggctg ggccgtgatc accgacgagt acaaggtgcc ctcaaagaag
      901 ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga agaatctcat tggcgctctc
      961 ctgttcgaca gcggcgagac ggctgaggct acgcggctca agcgcaccgc ccgcaggcgg
     1021 tacacgcgca ggaagaatcg catctgctac ctgcaggaga ttttctccaa cgagatggcg
     1081 aaggttgacg attctttctt ccacaggctg gaggagtcat tcctcgtgga ggaggataag
     1141 aagcacgagc ggcatccaat cttcggcaac attgtcgacg aggttgccta ccacgagaag
     1201 taccctacga tctaccatct gcggaagaag ctcgtggact ccacagataa ggcggacctc
     1261 cgcctgatct acctcgctct ggcccacatg attaagttca ggggccattt cctgatcgag
     1321 ggggatctca acccggacaa tagcgatgtt gacaagctgt tcatccagct cgtgcagacg
     1381 tacaaccagc tcttcgagga gaaccccatt aatgcgtcag gcgtcgacgc gaaggctatc
     1441 ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc tgatcgccca gctgccgggc
     1501 gagaagaaga acggcctgtt cgggaatctc attgcgctca gcctggggct cacgcccaac
     1561 ttcaagtcga atttcgatct cgctgaggac gccaagctgc agctctccaa ggacacatac
     1621 gacgatgacc tggataacct cctggcccag atcggcgatc agtacgcgga cctgttcctc
     1681 gctgccaaga atctgtcgga cgccatcctc ctgtctgata ttctcagggt gaacaccgag
     1741 attacgaagg ctccgctctc agcctccatg atcaagcgct acgacgagca ccatcaggat
     1801 ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg agaagtacaa ggagatcttc
     1861 ttcgatcagt cgaagaacgg ctacgctggg tacattgacg gcggggcctc tcaggaggag
     1921 ttctacaagt tcatcaagcc gattctggag aagatggacg gcacggagga gctgctggtg
     1981 aagctcaatc gcgaggacct cctgaggaag cagcggacat tcgataacgg cagcatccca
     2041 caccagattc atctcgggga gctgcacgct atcctgagga ggcaggagga cttctaccct
     2101 ttcctcaagg ataaccgcga gaagatcgag aagattctga ctttcaggat cccgtactac
     2161 gtcggcccac tcgctagggg caactcccgc ttcgcttgga tgacccgcaa gtcagaggag
     2221 acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg gcgctagcgc tcagtcgttc
     2281 atcgagagga tgacgaattt cgacaagaac ctgccaaatg agaaggtgct ccctaagcac
     2341 tcgctcctgt acgagtactt cacagtctac aacgagctga ctaaggtgaa gtatgtgacc
     2401 gagggcatga ggaagccggc tttcctgtct ggggagcaga agaaggccat cgtggacctc
     2461 ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca aggaggacta cttcaagaag
     2521 attgagtgct tcgattcggt cgagatctct ggcgttgagg accgcttcaa cgcctccctg
     2581 gggacctacc acgatctcct gaagatcatt aaggataagg acttcctgga caacgaggag
     2641 aatgaggata tcctcgagga cattgtgctg acactcactc tgttcgagga ccgggagatg
     2701 atcgaggagc gcctgaagac ttacgcccat ctcttcgatg acaaggtcat gaagcagctc
     2761 aagaggagga ggtacaccgg ctgggggagg ctgagcagga agctcatcaa cggcattcgg
     2821 gacaagcagt ccgggaagac gatcctcgac ttcctgaaga gcgatggctt cgcgaaccgc
     2881 aatttcatgc agctgattca cgatgacagc ctcacattca aggaggatat ccagaaggct
     2941 caggtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc tggctcgcca
     3001 gctatcaaga aggggattct gcagaccgtg aaggttgtgg acgagctggt gaaggtcatg
     3061 ggcaggcaca agcctgagaa catcgtcatt gagatggccc gggagaatca gaccacgcag
     3121 aagggccaga agaactcacg cgagaggatg aagaggatcg aggagggcat taaggagctg
     3181 gggtcccaga tcctcaagga gcacccggtg gagaacacgc agctgcagaa tgagaagctc
     3241 tacctgtact acctccagaa tggccgcgat atgtatgtgg accaggagct ggatattaac
     3301 aggctcagcg attacgacgt cgatcatatc gttccacagt cattcctgaa ggatgactcc
     3361 attgacaaca aggtcctcac caggtcggac aagaaccggg gcaagtctga taatgttcct
     3421 tcagaggagg tcgttaagaa gatgaagaac tactggcgcc agctcctgaa tgccaagctg
     3481 atcacgcagc ggaagttcga taacctcaca aaggctgaga ggggcgggct ctctgagctg
     3541 gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac ggcagatcac taagcacgtt
     3601 gcgcagattc tcgactcacg gatgaacact aagtacgatg agaatgacaa gctgatccgc
     3661 gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg acttcaggaa ggatttccag
     3721 ttctacaagg ttcgggagat caacaattac caccatgccc atgacgcgta cctgaacgcg
     3781 gtggtcggca cagctctgat caagaagtac ccaaagctcg agagcgagtt cgtgtacggg
     3841 gactacaagg tttacgatgt gaggaagatg atcgccaagt cggagcagga gattggcaag
     3901 gctaccgcca agtacttctt ctactctaac attatgaatt tcttcaagac agagatcact
     3961 ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga cgaacggcga gacgggggag
     4021 atcgtgtggg acaagggcag ggatttcgcg accgtcagga aggttctctc catgccacaa
     4081 gtgaatatcg tcaagaagac agaggtccag actggcgggt tctctaagga gtcaattctg
     4141 cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg actgggatcc gaagaagtac
     4201 ggcgggttcg acagccccac tgtggcctac tcggtcctgg ttgtggcgaa ggttgagaag
     4261 ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg ggatcacgat tatggagcgc
     4321 tccagcttcg agaagaaccc gatcgatttc ctggaggcga agggctacaa ggaggtgaag
     4381 aaggacctga tcattaagct ccccaagtac tcactcttcg agctggagaa cggcaggaag
     4441 cggatgctgg cttccgctgg cgagctgcag aaggggaacg agctggctct gccgtccaag
     4501 tatgtgaact tcctctacct ggcctcccac tacgagaagc tcaagggcag ccccgaggac
     4561 aacgagcaga agcagctgtt cgtcgagcag cacaagcatt acctcgacga gatcattgag
     4621 cagatttccg agttctccaa gcgcgtgatc ctggccgacg cgaatctgga taaggtcctc
     4681 tccgcgtaca acaagcaccg cgacaagcca atcagggagc aggctgagaa tatcattcat
     4741 ctcttcaccc tgacgaacct cggcgcccct gctgctttca agtacttcga cacaactatc
     4801 gatcgcaaga ggtacacaag cactaaggag gtcctggacg cgaccctcat ccaccagtcg
     4861 attaccggcc tctacgagac gcgcatcgac ctgtctcagc tcgggggcga caagcggcca
     4921 gcggcgacga agaaggcggg gcaggcgaag aagaagaagg caggctccgg tgggagcggc
     4981 ggttccggcg gctcgacgaa tttgtcagac attattgaaa aggagacggg gaagcaactc
     5041 gtgattcaag aatcgatact catgttgccc gaagaagttg aggaggtgat tgggaacaag
     5101 cctgagtcgg atatactggt ccacaccgct tatgacgaat ccaccgacga gaacgtcatg
     5161 cttctcacct cagacgcacc agagtataag ccttgggcac ttgtcataca agatagtaac
     5221 ggggagaata agatcaaaat gttgagcgga ggtagcggtg gaagtggagg ctccacgaat
     5281 ttgagcgaca taatagaaaa agaaacgggg aaacagctgg tgatacagga gtcaattttg
     5341 atgcttcccg aggaggtgga agaggtcatc ggtaataagc ccgagagtga tatactggtg
     5401 cataccgcgt acgatgaaag tactgatgag aacgtcatgt tgctcacaag cgatgctcct
     5461 gagtacaagc catgggcctt ggtcattcaa gattcgaacg gggaaaataa gataaagatg
     5521 ctgagtgggg ggagtaagcg cacggcagac ggatcagaat ttgagcctaa gaaaaagaga
     5581 aaagtttaat ggcggccgcc gacgtccgat cgttcaaaca tttggcaata aagtttctta
     5641 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     5701 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     5761 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     5821 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga
     5881 cagcttccgg aaagggcgaa ttcgcaactt tgtatacaaa agttgaacga gaaacgtaaa
     5941 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     6001 gtaaaacaca acatatccag tcactatgcc atccagctga tatcccctat agtgagtcgt
     6061 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     6121 acattgcaca agataaaaat atatcatcat gcctcctctg gaccagccag gacagaaatg
     6181 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     6241 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     6301 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     6361 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     6421 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     6481 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     6541 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     6601 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     6661 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     6721 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     6781 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     6841 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     6901 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     6961 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     7021 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     7081 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     7141 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     7201 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     7261 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     7321 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     7381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7981 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8041 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8101 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8161 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8221 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     8281 cgcgtaccgc gagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     8341 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     8401 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     8461 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     8521 ttgatgcctg gcagttccct actctcgcgt taacgcttgc atggatgttt tcccagtcac
     8581 gacgttgtaa aacgacggcc agtcttaagc tcg
//
