LOCUS       sequence-426760-        8499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT,
            CD81.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, 
            Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
  TITLE     Engineered CRISPR-Cas12a for higher-order combinatorial chromatin 
            perturbations.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
  PUBMED    38760567
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0."
COMMENT     Sequence Label: gCH198
            (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFO...
FEATURES             Location/Qualifiers
     source          1..8499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             792..972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1050..1171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1087..1106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1141..1160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1211..1346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1367..1385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1395..1412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1395..1411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1404..1426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1553..2008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1640..1659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1850..1871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2034..2138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2139..2207,2208..2999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2357..2376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3170..3758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3659..3678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3912..3929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4046..4067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4082..4087,4088..4105,4106..4112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4120..4136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4125..4147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4144..4160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4144..4160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4179..4199
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4181..4199
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4227..4453
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4454..4634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4681..4806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5299..5532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5717..5761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5942..6059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6143..6354
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6374..6383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6383..6406
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6407..6409,6410..6412,6413..7123)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6407..6409,6410..6412,6413..7123)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6452..6473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6713..6732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7060..7081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7133..7189
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7190..7789
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7190..7209)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7686..7706
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7805..8393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7858..7878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8276..8287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagt ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
      301 atttctactc ttgtagatac tggtattgcg gagccacgag gaatttctac tgtcgtagat
      361 atataagtgg aggcgtcgcg ctgaattcct actattgtag gtaggaatgc ccgccagcgc
      421 gacgcaattc ctcctcttgg aggtggctct gcaacaccgc ctagaccaat ttctactatt
      481 gtagatgctc cagacctggg gtccagttta attcctccta taggaggtcg ggaggccgca
      541 cccaccgcct gaatttctcc tataggagat ttcttcatcc ctagccagcc gccaattcct
      601 actctagtag gttctgcgtt ctgctcctac tgcttaattc ctactctcgt aggtagctct
      661 cgcccaagtg cagcgagaat ttctactcta gtagatggcg cgacccccag gaaggtctca
      721 atttctcctc taggagattt tttttaagct tggcgtaact agatcttgag acactgcttt
      781 ttgcttgtac tgggtctctc ttgttagacc agatctgagc ctgggagctc tctggctaac
      841 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      901 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      961 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     1021 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     1081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     1201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1321 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgtccta tagtgagtcg
     1381 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     1561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1981 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     2041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3961 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4021 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     4081 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     4141 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     4201 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     4261 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     4321 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     4381 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     4441 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4501 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4561 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4621 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     4681 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4741 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4801 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4861 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     4921 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     4981 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     5041 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     5101 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     5161 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5221 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5281 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5341 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5401 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5461 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5521 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5581 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5641 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5701 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5761 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5821 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5881 gcagggatat tcaccattat cgtttcagaa tcttgagaca aatggcagta ttcatccaca
     5941 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     6001 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     6061 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggtcg
     6121 agtggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6181 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6241 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6301 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg
     6361 tgacgcggga tccgccacca tggattacaa agacgatgac gataagatgg tgagcaaggg
     6421 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6481 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     6541 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     6601 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     6661 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     6721 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     6781 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6841 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6901 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     6961 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7021 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7081 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcagcg gcgccaccaa
     7141 cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta
     7201 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     7261 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     7321 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7381 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7441 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7501 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7561 cctggccacc gtcggcgtgt cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7621 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7681 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7741 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     7801 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7861 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7921 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7981 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8041 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8101 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8161 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8221 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8281 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     8401 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8461 ggactggaag ggctaattca ctcccaacga agacaagat
//
