LOCUS       sequence-426767-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA array targeting CD81, B2M, KIT, CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, 
            Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
  TITLE     Engineered CRISPR-Cas12a for higher-order combinatorial chromatin 
            perturbations.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
  PUBMED    38760567
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0."
COMMENT     Sequence Label: gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD8...
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             582..762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    840..961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(877..896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     931..950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1001..1136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1157..1175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1185..1202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1185..1201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1194..1216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1343..1798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1430..1449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1640..1661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1824..1928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1929..1997,1998..2789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2147..2166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2960..3548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3449..3468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3702..3719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3836..3857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3872..3877,3878..3895,3896..3902)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3910..3926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3915..3937
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3934..3950
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3934..3950
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3969..3989
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3971..3989
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4017..4243
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4244..4424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4471..4596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5089..5322
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5507..5551
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5732..5849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5933..6144
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6164..6173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6173..6196
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6197..6199,6200..6202,6203..6913)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6197..6199,6200..6202,6203..6913)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6242..6263)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6503..6522)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6850..6871
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6923..6979
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6980..7774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7034..7053)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7644..7663
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7790..8378
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7843..7863)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8261..8272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagt ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
      301 atttctactc ttgtagatac tggtattgcg gagccacgag gaatttctac tgtcgtagat
      361 atataagtgg aggcgtcgcg ctgaattcct actattgtag gttctgcgtt ctgctcctac
      421 tgcttaattc ctactctcgt aggtagctct cgcccaagtg cagcgagaat ttctactcta
      481 gtagatggcg cgacccccag gaaggtctca atttctcctc taggagattt tttttaagct
      541 tggcgtaact agatcttgag acactgcttt ttgcttgtac tgggtctctc ttgttagacc
      601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
      781 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
      841 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      901 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      961 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     1021 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1081 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1141 gacgtaccca attcgtccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     1201 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1261 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1321 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1381 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1441 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1501 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1561 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1621 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1681 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1741 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     1801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1921 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1981 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2041 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2101 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2161 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2221 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2281 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2341 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2401 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2461 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2521 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2581 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2641 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2701 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2761 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2821 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2881 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3001 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3061 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3721 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3781 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3841 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     3901 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     3961 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     4021 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     4081 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     4141 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     4201 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     4261 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4321 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4381 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     4441 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     4501 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     4561 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     4621 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     4681 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     4741 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     4801 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     4861 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     4921 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     4981 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     5041 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     5101 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     5161 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     5221 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     5281 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     5341 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     5401 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     5461 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     5521 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     5581 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     5641 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagaa
     5701 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     5761 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     5821 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     5881 tttggcgccg gctcgagggg gcccgggtcg agtggctccg gtgcccgtca gtgggcagag
     5941 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     6001 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6061 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     6121 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggattacaa
     6181 agacgatgac gataagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6241 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     6301 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6361 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     6421 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6481 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6541 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6601 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     6661 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     6721 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     6781 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     6841 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     6901 cgagctgtac aagggcagcg gcgccaccaa cttcagcctg ctgaagcagg ccggcgacgt
     6961 ggaggagaac cccggcccca tgcttgaaca agatggattg cacgcaggtt ctccggccgc
     7021 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7081 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7141 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7201 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7261 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7321 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7381 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7441 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7501 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7561 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7621 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7681 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7741 cgccttctat cgccttcttg acgagttctt ctgaacgcgt taagtcgaca atcaacctct
     7801 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7861 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7921 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7981 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8041 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8101 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8161 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     8221 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8281 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8341 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     8401 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8461 attcactccc aacgaagaca agat
//
