LOCUS       sequence-426788-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian
            cells from an inducible lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: FLAG-D1-IRES-SS-D2-IRES-HA-D3
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     39..61
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     58..74
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     58..74
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     93..113
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        95..113
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        141..367
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             368..548
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    595..720
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1213..1446
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1631..1675
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1941..2058
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2067..2087
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2093..2111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2129..2147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2165..2183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2237..2255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2273..2291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2309..2327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2372..2396
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2463..2487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2488..2497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2509..2532
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3155..3164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(3443..4017,4018..4020,4021..4029)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             join(4042..4047,4048..4071,4072..4107,4108..4131)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     protein_bind    complement(5008..5032)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5033..5607,5608..5610,5611..5619)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5632..5658
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6559..6579)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6592..7180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6645..6665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7063..7074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7211..7710
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7246..7269)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7643..7662
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7727..8326
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7727..7746)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8223..8243
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8398..8631
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8703..8824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8740..8759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8794..8813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8864..8999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9019..9038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9020..9038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9048..9065)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9048..9064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9057..9079)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9206..9661
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9293..9312)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9503..9524
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9687..9791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9792..9860,9861..10652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10010..10029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10823..11411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11312..11331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11565..11582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11699..11720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11735..11739,1,2..19,20..26)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
       61 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
      121 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
      181 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      241 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      301 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      361 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      541 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      601 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      661 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      721 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      781 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      841 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      901 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      961 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1021 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1081 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1141 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1201 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1261 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1321 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1381 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1441 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1501 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1561 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1621 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1681 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1741 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1801 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1861 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1921 atctcgacgg tatcgctagc ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     1981 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2041 acaaaaattc aaaattttac tagtatcaac tttgtataga aaagttgttt actccctatc
     2101 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     2161 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     2221 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     2281 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     2341 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     2401 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     2461 aaacaagttt gtacaaaaaa gcaggctgcc accatggtcg acggcggaga ctacaaggac
     2521 gacgatgaca agggcggagg cagcggagga ggatcagaac accagctcct gtgctgcgaa
     2581 gtggaaacca tccgccgcgc gtaccccgat gccaacctcc tcaacgaccg ggtgctgcgg
     2641 gccatgctga aggcggagga gacctgcgcg ccctcggtgt cctacttcaa atgtgtgcag
     2701 aaggaggtcc tgccgtccat gcggaagatc gtcgccacct ggatgctgga ggtctgcgag
     2761 gaacagaagt gcgaggagga ggtcttcccg ctggccatga actacctgga ccgcttcctg
     2821 tcgctggagc ccgtgaaaaa gagccgcctg cagctgctgg gggccacttg catgttcgtg
     2881 gcctctaaga tgaaggagac catccccctg acggccgaga agctgtgcat ctacaccgac
     2941 aactccatcc ggcccgagga gctgctgcaa atggagctgc tcctggtgaa caagctcaag
     3001 tggaacctgg ccgcaatgac cccgcacgat ttcattgaac acttcctctc caaaatgcca
     3061 gaggcggagg agaacaaaca gatcatccgc aaacacgcgc agaccttcgt tgccctctgt
     3121 gccacagatg tgaagttcat ttccaatccg ccctccatgg tggcagcggg gagcgtggtg
     3181 gccgcagtgc aaggcctgaa cctgaggagc cccaacaact tcctgtccta ctaccgcctc
     3241 acacgcttcc tctccagagt gatcaagtgt gacccggact gcctccgggc ctgccaggag
     3301 cagatcgaag ccctgctgga gtcaagcctg cgccaggccc agcagaacat ggaccccaag
     3361 gccgccgagg aggaggaaga ggaggaggag gaggtggacc tggcttgcac acccaccgac
     3421 gtgcgggacg tggacatctg agcccctctc cctccccccc ccctaacgtt actggccgaa
     3481 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     3541 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     3601 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3661 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3721 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3781 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3841 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3901 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3961 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     4021 gccacaacca tgggaggagg cagcgcctgg agccaccctc agttcgagaa gggaggagga
     4081 agcggcggag gcagcggagg aagcgcctgg agccacccgc agttcgagaa aggaggagga
     4141 gagctgctgt gccacgaggt ggacccggtc cgcagggccg tgcgggaccg caacctgctc
     4201 cgagacgacc gcgtcctgca gaacctgctc accatcgagg agcgctacct tccgcagtgc
     4261 tcctacttca agtgcgtgca gaaggacatc caaccctaca tgcgcagaat ggtggccacc
     4321 tggatgctgg aggtctgtga ggaacagaag tgcgaagaag aggtcttccc tctggccatg
     4381 aattacctgg accgtttctt ggctggggtc ccgactccga agtcccatct gcaactcctg
     4441 ggtgctgtct gcatgttcct ggcctccaaa ctcaaagaga ccagcccgct gaccgcggag
     4501 aagctgtgca tttacaccga caactccatc aagcctcagg agctgctgga gtgggaactg
     4561 gtggtgctgg ggaagttgaa gtggaacctg gcagctgtca ctcctcatga cttcattgag
     4621 cacatcttgc gcaagctgcc ccagcagcgg gagaagctgt ctctgatccg caagcatgct
     4681 cagaccttca ttgctctgtg tgccaccgac tttaagtttg ccatgtaccc accgtcgatg
     4741 atcgcaactg gaagtgtggg agcagccatc tgtgggctcc agcaggatga ggaagtgagc
     4801 tcgctcactt gtgatgccct gactgagctg ctggctaaga tcaccaacac agacgtggat
     4861 tgtctcaaag cttgccagga gcagattgag gcggtgctcc tcaatagcct gcagcagtac
     4921 cgtcaggacc aacgtgacgg atccaagtcg gaggatgaac tggaccaagc cagcacccct
     4981 acagacgtgc gggatatcga cctgtgaacc cagctttctt gtacaaagtg ggcccctctc
     5041 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5101 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5161 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5221 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5281 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5341 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5401 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5461 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5521 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5581 ttttcctttg aaaaacacga tgataatatg gccacaacca tgggaggcgg atacccatac
     5641 gacgtcccag actacgctgg cggaggcagc ggaggaggat cagagctgct gtgttgcgaa
     5701 ggcacccggc acgcgccccg ggccgggccg gacccgcggc tgctggggga ccagcgtgtc
     5761 ctgcagagcc tgctccgcct ggaggagcgc tacgtacccc gcgcctccta cttccagtgc
     5821 gtgcagcggg agatcaagcc gcacatgcgg aagatgctgg cttactggat gctggaggta
     5881 tgtgaggagc agcgctgtga ggaggaagtc ttccccctgg ccatgaacta cctggatcgc
     5941 tacctgtctt gcgtccccac ccgaaaggcg cagttgcagc tcctgggtgc ggtctgcatg
     6001 ctgctggcct ccaagctgcg cgagaccacg cccctgacca tcgaaaaact gtgcatctac
     6061 accgaccacg ctgtctctcc ccgccagttg cgggactggg aggtgctggt cctagggaag
     6121 ctcaagtggg acctggctgc tgtgattgca catgatttcc tggccttcat tctgcaccgg
     6181 ctctctctgc cccgtgaccg acaggccttg gtcaaaaagc atgcccagac ctttttggcc
     6241 ctctgtgcta cagattatac ctttgccatg tacccgccat ccatgatcgc cacgggcagc
     6301 attggggctg cagtgcaagg cctgggtgcc tgctccatgt ccggggatga gctcacagag
     6361 ctgctggcag ggatcactgg cactgaagtg gactgcctgc gggcctgtca ggagcagatc
     6421 gaagctgcac tcagggagag cctcagggaa gcctctcaga ccagctccag cccagcgccc
     6481 aaagcccccc ggggctccag cagccaaggg cccagccaga ccagcactcc tacagatgtc
     6541 acagccatac acctgtgaca actttattat acatagttga tcaattccga taatcaacct
     6601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6961 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     7021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7141 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc
     7201 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     7261 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     7321 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     7381 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     7441 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     7501 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     7561 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     7621 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     7681 ttctcctctt cctcatctcc gggcctttcg acctcacgtg gccaccatga ccgagtacaa
     7741 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7801 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7861 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7921 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7981 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8041 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8101 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8161 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8221 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8281 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggta cctttaagac
     8341 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8401 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8641 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8701 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8821 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     8881 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8941 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9001 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9061 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9181 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9241 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9301 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9361 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9421 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9481 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9541 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9601 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9661 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9841 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9901 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9961 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10021 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10081 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10141 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10201 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10261 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10321 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10381 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10441 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10501 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10561 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10621 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10681 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10741 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10801 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10861 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10921 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10981 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11041 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11101 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11161 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11221 aaagcgccac gcttcccgaa gagagaaagg cggacaggta tccggtaagc ggcagggtcg
    11281 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11341 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11401 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11461 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11521 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11581 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11641 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11701 atgtgagtta gctcactcat taggcacccc aggctttac
//
