LOCUS       sequence-42686-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL 25Q+ProCFPp303
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     315..331
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     316..332
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(397..838)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(403..426)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    721..838
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cgctctcgcc cttgctccca gggatccagg tcggtgcaga ggctcctcag ccacagctgg
       61 gcctggagga ggtggaggtg gaggtggagg aggttgtggc tgaggcagca gaggctgtgc
      121 ctgtggagga ggttgaggaa gttgaggagg tgggggtgga gggggaggtg gtggcggttg
      181 ttgctgttgc tgctgttgtt gctgttgctg ctgttgttgc tgttgctgct gttgttgctg
      241 ttgctgctgt tggaagcttt tgagggactc gaaggccttc atcagctttt ccagggtcgc
      301 catggcggtc gacctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
      361 cgggggatcc actagttcta gaatccgggg ttttttctcc ttgacgttaa agtatagagg
      421 tatattaaca attttttgtt gatactttta ttacatttga ataagaagta atacaaaccg
      481 aaaatgttga aagtattagt taaagtggtt atgcagtttt tgcatttata tatctgttaa
      541 tagatcaaaa atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat
      601 cgcattatca tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg
      661 ttactgccaa tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt
      721 cggagcagtg cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga
      781 cgcacggagg agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta
      841 ctaaagctcc aattcgccct atagtgagtc gtatt
//
