LOCUS       sequence-426907-        9732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An expression vector for mouse LINE1 ORF1-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Zheng F, Kawabe Y, Murakami M, Takahashi M, Nishihata K, Yoshida S, 
            Ito A, Kamihira M
  TITLE     LINE-1 vectors mediate recombinant antibody gene transfer by 
            retrotransposition in Chinese hamster ovary cells.
  JOURNAL   Biotechnol J. 2021 Jul;16(7):e2000620. doi: 10.1002/biot.202000620. 
            Epub 2021 May 20.
  PUBMED    33938150
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2021-07"; volume: "16(7):e2000620. doi"; pages: " 
            10.1002/biot.202000620. Epub 2021 May 20"
COMMENT     Sequence Label: pORF1-2
FEATURES             Location/Qualifiers
     source          1..9732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..37)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        22..351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      202..337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     401..420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        401..419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(5469..6043,6044..6046,6047..6055)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5636..5653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5863..5882
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6056..6733
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6186..6204)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6236..6256)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6454..6473
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6648..6671
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6777..6794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6783..7007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7090..7678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7170..7189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7769..7787
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7825..7847)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7947..7966
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8033..8488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8120..8139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8330..8351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        8524..8827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8828..9031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8982..9002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9028..9052
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        9149..9196
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9215..9589
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    join(9603..9732,1..5)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9640..9659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9694..9713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggatcgatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
       61 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctctct
      361 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      421 agacccaagc tggctagcgc taccggactc agatctacca tggccaaggg caagcgccgc
      481 aacctgacca accgcaacca ggaccacagc cccagccccg agcccagcac ccccaccagc
      541 cccagccccg gcaaccccaa cacccccgag aacctggacc tggacctgaa ggcctacctg
      601 atgatgatgg tggaggacat caagaaggac ttcaacaaga gcctgaagga gatccaggag
      661 aacaccgcca aggagctgca ggtgctgaag gagaagcagg agaacaccat caagcaggtg
      721 gaggtgctga ccgagaagga ggagaagacc tacaagcagg tgatggagat gaacaagacc
      781 atcctggacc tgaagcgcga ggtcgacacc atcaagaaga cccagagcga ggccaccctg
      841 gagatcgaga ccctgggcaa gaagagcggc accatcgacc tgagcatcag caaccgcatc
      901 caggagatgg aggagcgcat cagcggcgcc gaggacagca tcgagaacat cggcaccacc
      961 atcaaggaga acggcaagtg caagaagatc ctgacccaga acatccagga gatccaggac
     1021 accatccgcc gccccaacgt gcgcatcatc ggcgtggacg agaacgagga cttccagctg
     1081 aagggccccg ccaacatctt caacaagatc atcgaggaga acttccccaa cctgaagaac
     1141 gagatgcaca tgaacatcca ggaggcctac cgcaccccca accgcctgga ccagaagcgc
     1201 aacagctcta gacacatcat catccgcacc agcaacgccc tgaacaagga ccgcatcctg
     1261 aaggccgtgc gcgagaaggg ccaggtgacc tacaagggca agcccatccg catcaccccc
     1321 gacttcagcc ccgagaccat gaaggcccgc cgcgcctgga ccgacgtgat ccagaccctg
     1381 cgcgagcaca agctgcagcc ccgcctgctg taccccgcca agctgagcat catcatcgag
     1441 ggcgagacca aggtgttcca cgacaagacc aagttcaccc actacctgag caccaacccc
     1501 gccctgcagc gcatcatcac cgagaagaac cagtacaaga acggcaacaa cgccctggag
     1561 aagacccgcc gctaatccct caacaaacct taattaagac agccacaaga acagaatgcc
     1621 ccccctgacc accaagatca ccggcagcaa caactacttc agcctgatca gcctgaacat
     1681 caacggcctg aacagcccca tcaagcggca ccgcctgacc aactggctgc acaagcagga
     1741 ccccaccttc tgttgcctcc aggagaccca cctgcgcgag aaggaccggc actacctgcg
     1801 gatgaagggc tggaagacca tctttcaggc caacggcatg aagaagcagg ctggcgtggc
     1861 catcctgatc agcgacaaga tcgacttcca gcccaaggtg atcaagaagg acaaggaggg
     1921 ccacttcatc ctgatcaagg gcaagatcct gcaggaggag ctgagcattc tgaacatcta
     1981 cgcccccaac acccgcgccg ccaccttcac caaggagacc ctcgtgaagc tgaaggccca
     2041 catcgctccc cacaccatca tcgtcggcga cttcaacacc cccctgagcc ccatggacag
     2101 atcttggaag cagaagctga accgcgacac cctgaagctg accgaggtga tgaagcagat
     2161 ggacctgacc gacatctacc gcaccttcta ccccaagacc aagggctaca ccttcttcag
     2221 cgctccccac ggcaccttca gcaagatcga ccacatcatc ggccacaaga gcgggctgaa
     2281 ccggctgaag aacatcgaga tcgtgccctg catcctgagc gaccaccatg ccctgcgcct
     2341 gatcttcaac aacaagatta acaaccgcaa gcccaccttc acctggaagc tgaacaacac
     2401 cctgctgaac gacaccctgg tgaaggaagg catcaagaag gagatcaagg acttcctgga
     2461 gttcaacgag aacgaggcca ccacctaccc taacctgtgg gacaccatga aggccttcct
     2521 gcggggcaag ctgatcgcca tgagcgcctt caagaagaag cgggagcgcg cccacactag
     2581 tagcctgacc acccacctga aggccctgga gaagaaggag gctaactccc ccaagcgctc
     2641 ccggcggcag gagatcatca agctgcgcgg cgagatcaac caggtggaga cccggcgcac
     2701 catccaacgg atcaaccaga cccggtcttg gttcttcgag aagatcaaca agatcgacaa
     2761 gcccctggct cgcctgacca agggccaccg cgacaagatc ctgatcaaca agatccgcaa
     2821 cgagaagggc gacatcacca ccgaccccga ggagatccag aacaccatcc gcagcttcta
     2881 caagcgcctg tacagcacca agctggagaa cctggacgag atggacaagt tcctggaccg
     2941 ctatcaggtg cccaagctga accaggacca ggtggacctg ctgaacagcc ccatctcccc
     3001 caaggaaatc gaggccgtga tcaacagcct gcccgccaag aagagccccg gccccgacgg
     3061 cttcagcgct gagttctacc agaccttcaa ggaggacctg acccccgtgc tgcacaagct
     3121 tttccacaag atcgaggtcg agggcatcct ccccaacagc ttctacgagg ccaccatcac
     3181 cctgatcccc aagccccaga aggaccccac caagatcgag aacttccgcc ccatcagcct
     3241 gatgaacatc gacgccaaga tcctgaacaa gatcctcgcc aaccgcatcc aggagcacat
     3301 caaggccatc atccaccctg accaggtcgg cttcatcccc ggcatgcagg gctggttcaa
     3361 catccgcaag agcatcaacg tgatccacta catcaacaag ctgaaggaca agaaccacat
     3421 gatcatcagc ctggacgccg agaaggcctt cgacaagatc cagcacccct tcatgatcaa
     3481 ggtcctggag cgcagcggca ttcagggcca gtacctcaac atgatcaagg ccatctacag
     3541 caagcccgtc gccaacatca aggtgaacgg ggagaagctg gaggccatcc ctctgaagag
     3601 cggcacgcgt cagggctgtc ctctgtcccc ctacctgttc aacatcgtgc tggaggtgct
     3661 ggcccgcgct atccggcagc agaaggagat caagggcatc cagatcggca aggaggaagt
     3721 gaagatcagc ctgttcgccg acgacatgat cgtgtacatc agcgacccca agaacagcaa
     3781 ccgcgagctc ctgaacctga tcaacagctt cggcgaggtg gctggctaca agatcaacag
     3841 caacaagagc atggccttcc tgtacaccaa gaacaagcag gccgagaagg agatccgcga
     3901 gaccaccccc ttcagcatcg ccaccaacaa catcaagtac ctgggcgtga ccctgaccaa
     3961 ggaggtgaag gacctgtacg acaagaactt caagagcctg aagaaggaga tcaaggagga
     4021 cctgcgccgc tggaaggacc tgccctgcag ctggatcggc cgcaccaaca tcgtgaagat
     4081 ggccatcctg cccaaggcta tctaccgctt caacgccatc cccatcaaga tccccaccca
     4141 gttcttcaac gagctcgagg gcgccatctg caagttcatc tggaacaaca agaagccccg
     4201 catcgccaag accctgctga aggacaagcg caccagtggg gggatcacca tgcccgacct
     4261 gaagctgtac taccgcgcca tcgtgatcaa gaccgcctgg tactggtacc gcgaccgcca
     4321 ggtggaccag tggaaccgca tcgaggaccc cgagatgaac ccccacacct acggccacct
     4381 gatcttcgac aagggcgcca agaccatcca gtggaagaag gacagcatct tcaacaactg
     4441 gtgctggcac aactggctgc tgagctgccg ccgcatgcgc atcgacccct acctaagccc
     4501 ctgcaccaag gtgaagagca agtggatcaa ggagctgcac atcaagcccg agactctgaa
     4561 gctgatcgag gagaaggtgg gcaagagcct ggaggacatg ggcaccggcg aaaagttcct
     4621 gaaccgcacc gccatggcct gtgccgtgcg cagccggatc gataagtggg acctgatgaa
     4681 gctgcagtcc ttctgtaagg ccaaggatac cgtgtacaag accaagcgcc ccccgaccga
     4741 ctgggagcgc atcttcacct accccaagag cgaccgcggc ctgatcagca acatctacaa
     4801 ggagctgaag aaggtggacc tgcgcaagag caacaacccg ctgaagaagt ggggctccga
     4861 gctgaacaag gagttctccc ccgaggagta ccgcatggcc gagaagcacc tgaagaagtg
     4921 cagcaccagc ctgatcatcc gcgagatgca gatcaagacc accctgcgct tccacctgac
     4981 ccccgtgcgg atggccaaga tcaagaacag cggcgactcg cgatgctggc ggggctgcgg
     5041 cgagcgcggc accctgctgc actgctggtg ggactgtcgc ctggtccagc ccctgtggaa
     5101 gagcgtgtgg cggttcctgc ggaagctgga catcgtgctg cccgaggacc ccgctatccc
     5161 cctgctgggc atctaccctg aggaggcccc caccggcaag aaggacacct gcagtaccat
     5221 gttcatcgcc gctctgttca tcatcgcccg caactggaag gagccccgct gccccagcac
     5281 cgaggagtgg attcagaaga tgtggtacat ctacaccatg gagtactaca gcgccatcaa
     5341 gaagaacgag ttcatgaagt tcctggccaa gtggatggac ctggagagca tcatcctgag
     5401 cgaggtgacc cagagccagc gcaacagcca caacatgtac agcctgatca gcggctacta
     5461 gggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5521 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5581 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5641 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5701 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5761 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5821 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5881 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5941 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc
     6061 ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt
     6121 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     6181 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     6241 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
     6301 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     6361 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggctccttca tctacaaggt
     6421 gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg
     6481 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     6541 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ctatctatat
     6601 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gactccaagc tggacatcac
     6661 ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcgccgagg gccgccacca
     6721 cctgttcctg tagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     6781 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6841 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6901 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6961 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7021 aagaaccagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7741 gattttggtc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     7801 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7861 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7921 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7981 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggacgcgccc
     8041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8461 tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgaac tagatctaga
     8521 gtccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8821 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8881 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8941 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9001 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     9061 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     9121 ccgggaacgg tgcattggaa cggaccgtgt tgacaattaa tcatcggcat agtatatcgg
     9181 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     9241 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9301 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9361 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9421 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9481 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9541 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac actcgacctc
     9601 gaaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9721 cttatcatgt ct
//
