LOCUS       sequence-426935-        9380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For integration of MYOD1 WT T2A mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Naderi J, Magalhaes AP, Kibar G, Stik G, Zhang Y, Mackowiak SD, 
            Wieler HM, Rossi F, Buschow R, Christou-Kent M, Alcoverro-Bertran M,
            Graf T, Vingron M, Hnisz D
  TITLE     An activity-specificity trade-off encoded in human transcription 
            factors.
  JOURNAL   Nat Cell Biol. 2024 Aug;26(8):1309-1321. doi: 
            10.1038/s41556-024-01411-0. Epub 2024 Jul 5.
  PUBMED    38969762
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01411-0"; journalName: "Nat Cell Biol"; date: 
            "2024-08"; volume: "26"; issue: "8"; pages: "1309-1321"
COMMENT     Sequence Label: pPB-TetON-mEGFP-MYOD1_WT
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   147..209
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        658..1036
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    666..684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    702..720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    738..756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    774..792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    810..828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    846..864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    882..900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     945..969
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1049..1058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1058..1123
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2084..2137
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2144..2146,2147..2857)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2144..2146,2147..2857)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2186..2207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2447..2466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2794..2815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2944..2963)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2988..3036
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3267..3478
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3491..3759
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3793..4536
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4537..4590
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4591..5190
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4591..4610)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5087..5107
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5223..5344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5260..5279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5314..5333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6107..6141
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6632..6648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6632..6648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6645..6667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6656..6672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6680..6686,6687..6704,6705..6710))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6725..6746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6863..6880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7034..7622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7114..7133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7793..8584,8585..8653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8416..8435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8654..8758)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8784..9239)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8921..8942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9133..9152
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9366..9380,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9380,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
       61 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
      121 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
      181 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
      241 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
      301 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
      361 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
      421 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
      481 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
      541 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
      601 ggggatacgg ggaaaaggcc tccacggcca ctagtctttc gtcttcaaga attcctcgag
      661 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
      721 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
      781 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
      841 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
      901 acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
      961 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     1021 ttcctaccct cgtaaaggtc tagagctagc caccatggac tacaaagacc atgacggtga
     1081 ttataaagat catgacatcg attacaagga tgacgatgac aagatggagc ttctctcacc
     1141 gcccctgagg gatgtggatt tgaccgctcc tgatggatcc ttgtgtagct tcgctacgac
     1201 agatgacttt tacgatgatc cttgctttga tagtcctgat cttcggttct ttgaggattt
     1261 ggatcctaga ctcatgcatg ttggagccct tcttaagcct gaggaacatt cccattttcc
     1321 ggctgccgtc catcccgcgc ccggagctag ggaagatgaa cacgttagag ccccttctgg
     1381 acatcatcaa gccgggagat gtttgttgtg ggcgtgtaaa gcctgtaaaa ggaaaactac
     1441 aaatgctgat aggcgcaaag cggctacaat gagggaacga cggcgactta gtaaggttaa
     1501 cgaagctttc gaaaccctga aacgatgtac ttccagtaac cccaatcaac gactgcctaa
     1561 agttgaaatt ctcagaaatg ctattcggta cattgaagga cttcaagcct tgttgcgaga
     1621 tcaagatgct gcaccccctg gagcagcggc agcgttttac gccccagggc cactcccgcc
     1681 cggtaggggt ggggaacatt attcaggtga ttctgatgcc agttctccaa gatcaaattg
     1741 ttctgatggg atgatggatt attccgggcc gccctctggt gcacgcagga gaaattgtta
     1801 tgagggtgca tattataatg aagccccgtc agagcctcgc cctggcaaat ctgccgctgt
     1861 atcctcactt gattgtcttt caagtattgt cgaaagaatt agcactgaaa gtcccgcagc
     1921 accggcactg ttgctcgccg atgtaccatc cgaaagccca cctcgaaggc aagaagccgc
     1981 ggctccatca gaaggagaaa gttccggtga tcctactcaa tcaccggacg ccgccccgca
     2041 gtgccctgcg ggtgcgaacc ccaacccgat ataccaggtg ctcgagggca gaggaagtct
     2101 tctaacatgc ggtgacgtgg aggagaatcc cggccctagt accgtgagca agggcgagga
     2161 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2221 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2281 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2341 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2401 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2461 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2521 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2581 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2641 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2701 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2761 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2821 cgccgggatc actctcggca tggacgagct gtacaagacc ggttgaccgg ttagtaatga
     2881 gtttaaacgg gggaggaaga ccggttgacc ggttagtaat gagtttaaac gggggaggct
     2941 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     3001 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     3061 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     3121 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     3181 gggcggcagg ccctgccata gcagatctgc gaaatcggat ccagctgaat ctaagtggat
     3241 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3301 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     3361 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3421 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     3481 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     3541 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     3601 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     3661 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     3721 cgtctttgtt tcgttttctg ttctgcgccg ttacagatct aagctgtgac cggcgcctac
     3781 tctagagccg ccatgtctag actggacaag agcaaagtca taaactctgc tctggaatta
     3841 ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     3901 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     3961 atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga gtcatggcaa
     4021 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     4081 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     4141 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     4201 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     4261 gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc aattgagctg
     4321 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     4381 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac
     4441 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     4501 gctcttgacg attttgacct tgacatgctc cccggggagg gcagaggaag tcttctaaca
     4561 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     4621 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4681 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     4741 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4801 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4861 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4921 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     4981 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5041 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5101 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5161 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtggg
     5221 taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5341 cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag ctcatctggt
     5401 ctcccttccg ggggacaaga catccctgtt taatatttaa acagcagtgt tcccaaactg
     5461 ggttcttata tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac
     5521 gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg gattcctttt
     5581 ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca ggctcaaaga
     5641 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc
     5701 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc
     5761 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg
     5821 ggggctgtcc ctgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     5881 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     5941 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     6001 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     6061 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     6121 acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt
     6181 ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt
     6241 gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc
     6301 attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac
     6361 gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc
     6421 cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac
     6481 agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac
     6541 gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag
     6601 ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8761 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     8821 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8881 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8941 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     9001 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     9061 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     9121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     9181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     9241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9361 gttttcccag tcacgacgtt
//
