LOCUS       sequence-427212-        1941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of improved GABA sensor (negative change in 
            fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Kolb I, Hasseman JP, Matsumoto A, Arthur BJ, Zhang Y, Tsang A, Reep 
            D, Tsegaye G, Zheng J, Patel R, Looger LL, Marvin JS, Korff WL, 
            Yonehara K, Turner GC
  TITLE     iGABASnFR2: Improved genetically encoded protein sensors of GABA
  JOURNAL   bioRxiv 2025.03.25.644953
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.25.644953"
COMMENT     Sequence Label: pGP-AAV-CAG-iGABASnFR2n-WPRE
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1107..1128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1231..1252)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             1759..1788
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1792..1938
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacagatctg aaagcatcaa tttcgtgagc tggggcggta gcacccagga tgcgcagaag
      121 caggcctggg ccgacccgtt cagcaaggcc agcggcatta ccgtggtcca ggatgggccc
      181 accgactacg gcaaactcaa ggccatggtc gaaagcggca acgtgcagtg ggacgtggtg
      241 gatgtagagg ccgacttcgc cttgcgcgcc gccgccgaag gcctgctcga acccctggat
      301 ttctcggtga tccagcgcga caagatcgat ccgcggttcg tgagtgacca tggagtgggg
      361 gccttcctgt tctctcacgt gctcggctac aacgaaggca agctcggagc cagcaagccc
      421 caggactgga ccgcgctgtt cgacaccaag acctaccccg gcaaacgcgc cctctacaaa
      481 tggccgagcc ctggcgtgct cgaactggcc ctgctcgccg acggcgtacc ggccgacaag
      541 ctctacccgc tggacctgga ccgcgccttc aagaaactcg acaccatcaa gaaagacatc
      601 gtctggtggg gcggcggtgc acagtcgcag cagctgctgg cctccggcga agtcagcatg
      661 ggccagttct ggaacggtcg catccacgcc ctgcaagaag acggcgcgcc agtgggcgtg
      721 agctggaagc agaacctggt gatggccgac atcctcgtcg tgcctaaagg cacgaagaac
      781 aaagccgccg cgatgaagtt cctggccagt gccagcagcg ccaaaggcca ggacgacttt
      841 tccgccctga ccgcctatgc tccagtgaac atcgacagtg tgcagcgcct cgaccttgcg
      901 caggtccgga tcaccgccga caagcagaag aacggcatca tggcgaactt caagatcccg
      961 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc
     1201 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1261 cacaagttca gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
     1321 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1381 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1441 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
     1501 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1561 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1621 tggaacgcca acctggcacc gaacctgccg accgcctacg tcaaggatca gatcaccctc
     1681 gatttcgcct actgggccaa gaacggtccg gccattgcga cacggtggaa tgaatggcta
     1741 gtcaaactgc aggtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc
     1801 caggacacgc aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc
     1861 tcagccatcc tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt
     1921 tggcagaaga agccacgtta a
//
