LOCUS       sequence-42722-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM
  TITLE     A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit
            protein translation depending upon cell context.
  JOURNAL   Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
  PUBMED    21518959
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-06"; volume: "31(12)"; pages: "2513-27. Epub 
            2011 Apr 25"
COMMENT     Sequence Label: pcDNA3.1 - HA-KLF4 FL
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             140..166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             140..166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccggcgag tctgacatgt acccctacga cgtgcccgac tacgccgctg tcagcgacgc
      181 gctgctccca tctttctcca cgttcgcgtc tggcccggcg ggaagagaga agacactgcg
      241 tcaagcaggt gccccgaata accgctggcg ggaggagctc tcccacatga agcgacttcc
      301 cccagtgctt cccggccgcc cctatgacct ggcggcggcg accgtggcca cagacctgga
      361 gagcggcgga gccggtgcgg cttgcggcgg tagcaacctg gcgcccctac ctcggagaga
      421 gaccgaggag ttcaacgatc tcctggacct ggactttatt ctctccaatt cgctgaccca
      481 tcctccggag tcagtggccg ccaccgtgtc ctcgtcagcg tcagcctcct cttcgtcgtc
      541 gccgtcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcacct atccgatccg
      601 ggccgggaac gacccgggcg tggcgccggg cggcacgggc ggaggcctcc tctatggcag
      661 ggagtccgct ccccctccga cggctccctt caacctggcg gacatcaacg acgtgagccc
      721 ctcgggcggc ttcgtggccg agctcctgcg gccagaattg gacccggtgt acattccgcc
      781 gcagcagccg cagccgccag gtggcgggct gatgggcaag ttcgtgctga aggcgtcgct
      841 gagcgcccct ggcagcgagt acggcagccc
//
