LOCUS       sequence-42724-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM
  TITLE     A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit
            protein translation depending upon cell context.
  JOURNAL   Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
  PUBMED    21518959
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-06"; volume: "31(12)"; pages: "2513-27. Epub 
            2011 Apr 25"
COMMENT     Sequence Label: pMIR-Report-Luc-KLF4-FL
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..266)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(92..112)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        267..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(691..710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(692..710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(722..740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(723..740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(760..776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(760..776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(773..795)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    784..800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(808..814,815..832,833..838))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    853..874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggatccacg gttcactaaa
       61 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      121 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      181 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      241 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
      301 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
      361 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
      421 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
      481 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
      541 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
      601 gaccccgtaa ttgattacta ttaataacta agatctggta ccttgaattc atgcttctcc
      661 tccctttagt gagggtaatt ctctctctct ccctatagtg agtcgtatta attccttctc
      721 ttctatagtg tcacctaaat cgttgcaatt cgtaatcatg tcatagctgt ttcctgtgtg
      781 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      841 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      901 ccagtcggga aaacctgtcg
//
