LOCUS       sequence-427371-       13665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic BiFC vector  (Gateway compatible).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 13665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-C-GW-P2A:mTagBFP2
FEATURES             Location/Qualifiers
     source          1..13665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1166..1417
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1354..1375
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1418..1444
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1463..1587
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2016..2321)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2118..2137)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2641..3324)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     3239..3258
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3325..3427)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3605..3729)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3737..3793
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3794..3814
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3815..3835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3836..3856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4587..4839
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4872..4888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4872..4888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4885..4907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4896..4912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4920..4926,4927..4944,4945..4950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4965..4986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5022..5274)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5425..6450)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6502..6681)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7187..7211
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7732..8523
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8769..9357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9258..9277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9511..9528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9543..9683
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9609..9628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9769..9791
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10027..10221
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10287..11360)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11789..12418)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggacaa gcagaagaac ggcatcaagg ccaacttcaa
     1201 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     1261 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1321 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1381 cgccgggatc actctcggca tggacgagct gtacaagtac ccatacgatg ttccagatta
     1441 cgctgggccc aggcctacta gtacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     1501 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     1561 taaaacacaa catatccagt cactatgaat caactactta gatggtatta gtgacctgta
     1621 gtcgaccgac agccttccaa atgttcttcg ggtgatgctg ccaacttagt cgaccgacag
     1681 ccttccaaat gttcttctca aacggaatcg tcgtatccag cctactcgct attgtcctca
     1741 atgccgtatt aaatcataaa aagaaataag aaaaagaggt gcgagcctct tttttgtgtg
     1801 acaaaataaa aacatctacc tattcatata cgctagtgtc atagtcctga aaatcatctg
     1861 catcaagaac aatttcacaa ctcttatact tttctcttac aagtcgttcg gcttcatctg
     1921 gattttcagc ctctatactt actaaacgtg ataaagtttc tgtaatttct actgtatcga
     1981 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
     2041 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
     2101 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
     2161 accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg
     2221 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
     2281 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
     2341 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
     2401 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
     2461 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
     2521 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
     2581 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt
     2641 ttagtaagcc ggatccacgc ggcgtttacg ccccccctgc cactcatcgc agtactgttg
     2701 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2761 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2821 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2881 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2941 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     3001 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     3061 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     3121 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     3181 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     3241 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     3301 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     3361 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     3421 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     3481 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     3541 tgcgtcgggt gatgctgcca acttagtcga ctacaggtca ctaataccat ctaagtagtt
     3601 gattcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     3661 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     3721 caaagtggtt ctcgaggcaa ccaactttag cctgctcaag caagcaggag atgttgagga
     3781 aaatcctggc ccccctaaga aaaagagaaa ggttccgaaa aagaaaagaa aggtgccaaa
     3841 aaaaaagaga aaggtaatgg tgagcaaggg cgaggagctt atcaaagaga atatgcacat
     3901 gaagttgtat atggagggta ctgtggataa ccatcacttt aaatgtacat cagaaggaga
     3961 gggaaagcct tacgagggta ctcaaacaat gagaattaag gttgtggagg gtggtcctct
     4021 tccatttgct ttcgatatct tggcaacatc ttttctctac ggttcaaaaa ctttcattaa
     4081 ccatacacag ggtattccag atttcttcaa gcagtctttt ccagagggat tcacttggga
     4141 aagagttact acttatgagg atggtggtgt tcttactgca actcaagata catctcttca
     4201 ggatggatgc ttgatctata acgttaagat cagaggagtt aatttcactt caaacggacc
     4261 tgttatgcaa aagaaaacat tgggttggga ggcttttact gagacattgt acccagcaga
     4321 tggaggtttg gaaggaagaa acgatatggc tcttaaattg gttggaggtt ctcatctcat
     4381 cgcaaatgct aaaactacat atagatcaaa gaaacctgct aagaacctca aaatgccagg
     4441 agtttattac gtggattaca gacttgaaag aattaaagag gcaaacaacg aaacttatgt
     4501 tgaacagcat gaggttgctg tggcaagata ctgtgatctt ccttctaaac tggggcacaa
     4561 acttaattaa gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     4621 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     4681 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4741 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4801 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa
     4861 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4981 acattaggct gaattaggcg cgcctatttc tattaattcc cgatctagta acatagatga
     5041 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     5101 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     5161 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     5221 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
     5281 attcggggga tcgatcccgc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt
     5341 tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca
     5401 gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg
     5461 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     5521 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     5581 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     5641 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     5701 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     5761 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     5821 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     5881 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     5941 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     6001 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     6061 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     6121 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     6181 gcaataggtc aggctctcgc tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc
     6241 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     6301 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     6361 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
     6421 agacgtcgcg gtgagttcag gctttttcat atcttattgc cccccgggat cagatctgga
     6481 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     6541 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     6601 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     6661 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     6721 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     6781 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     6841 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     6901 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     6961 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     7021 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     7081 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     7141 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     7201 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     7261 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     7321 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     7381 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     7441 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     7501 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     7561 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     7621 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     7681 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     7741 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     7801 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7861 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     7921 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     7981 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     8041 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     8101 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     8161 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     8221 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     8281 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     8341 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     8401 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     8461 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     8521 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     8581 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     8641 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     8701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9541 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     9601 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     9661 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     9721 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9781 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9841 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     9901 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     9961 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
    10021 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
    10081 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
    10141 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
    10201 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
    10261 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
    10321 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
    10381 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
    10441 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    10501 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    10561 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    10621 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    10681 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    10741 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    10801 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10861 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    10921 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    10981 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    11041 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    11101 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    11161 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    11221 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    11281 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    11341 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    11401 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    11461 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    11521 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    11581 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    11641 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    11701 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    11761 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    11821 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    11881 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    11941 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    12001 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    12061 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    12121 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    12181 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    12241 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    12301 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    12361 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    12421 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    12481 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    12541 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    12601 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    12661 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    12721 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    12781 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    12841 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    12901 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    12961 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    13021 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    13081 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    13141 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    13201 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    13261 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    13321 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    13381 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    13441 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    13501 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    13561 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    13621 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
