LOCUS       sequence-42748-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 1014 pcDNA3 T7 Akt1 K179M T308A S473A
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gctagcatga ctggtggaca gcaaatggga
      121 tccatgagcg acgtggctat tgtgaaggag ggttggctgc acaaacgagg ggagtacatc
      181 aagacctggc ggccacgcta cttcctcctc aagaatgatg gcaccttcat tggctacaag
      241 gagcggccgc aggatgtgga ccaacgtgag gctcccctca acaacttctc tgtggcgcag
      301 tgccagctga tgaagacgga gcggccccgg cccaacacct tcatcatccg ctgcctgcag
      361 tggaccactg tcatcgaacg caccttccat gtggagactc ctgaggagcg ggaggagtgg
      421 acaaccgcca tccagactgt ggctgacggc ctcaagaagc aggaggagga ggagatggac
      481 ttccggtcgg gctcacccag tgacaactca ggggctgaag agatggaggt gtccctggcc
      541 aagcccaagc accgcgtgac catgaacgag tttgagtacc tgaagctgct gggcaagggc
      601 actttcggca aggtgatcct ggtgaaggag aaggccacag gccgctacta cgccatgatg
      661 atcctcaaga aggaagtcat cgtagccaag gacgaagtgg cccacacact caccgagaac
      721 cgcgtcctgc agaactccag gcaccccttc ctcacagccc tgaagtactc tttccagacc
      781 cacgaccgcc tctgctttgt catggagtac gccaacgggg gcgagctgtt cttccacctg
      841 tcccgggagc gtgtgttctc cgaggaccgg gcccgc
//
