LOCUS       sequence-42751-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, 
            Heliot L, McNally JG
  TITLE     Concurrent fast and slow cycling of a transcriptional activator at 
            an endogenous promoter.
  JOURNAL   Science. 2008 Jan 25;319(5862):466-9.
  PUBMED    18218898
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2008-01-25"; volume: "319(5862)"; pages: "466-9"
COMMENT     Sequence Label: pTSK241
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..763)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccaatggacc agaactacct gtgaaattaa taacagacat ccgcggttat ttgtacaatt
       61 catccatacc atgggtaata ccagcagcag taacaaattc taacaagacc atgtggtctc
      121 tcttttcgtt tggatctttg gataaggcag attgagtgga taagtaatgg ttgtctggta
      181 acaagactgg accatcacca attggagtat tttgttgata atggtcagct aattgaacag
      241 aaccatcttc aatgttgtgt ctaattttga agttaacttt gataccattc ttttgtttgt
      301 cagccatgat gtaaacattg tgagagttat agttgtattc caatttgtga cctaaaatgt
      361 taccatcttc tttaaaatca atacctttta attcgattct attaactaag gtatcacctt
      421 caaacttgac ttcagctctg gtcttgtagt taccgtcatc tttgaaaaaa atagttcttt
      481 cttgaacata accttctggc atggcagact tgaaaaagtc atgttgtttc atatgatctg
      541 ggtatctcgc aaaacattga acaccataac cgaaagtagt gactaaggtt ggccatggaa
      601 ctggcaattt accagtagta caaataaatt ttaaggtcaa tttaccgtaa gtagcatcac
      661 cttcaccttc accggagaca gaaaatttgt gaccattaac atcaccatct aattcaacca
      721 aaattgggac aacaccagtg aataattctt cacctttaga cattctag
//
