LOCUS       sequence-42765-e         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Dinkins RD, Majee SM, Nayak NR, Martin D, Xu Q, Belcastro MP, Houtz 
            RL, Beach CM, Downie AB
  TITLE     Changing transcriptional initiation sites and alternative 5'- and 
            3'-splice site selection of the first intron deploys Arabidopsis 
            protein isoaspartyl methyltransferase2 variants to different 
            subcellular compartments.
  JOURNAL   Plant J. 2008 Jul;55(1):1-13.
  PUBMED    18318686
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2008-07"; volume: "55(1)"; pages: "1-13"
COMMENT     Sequence Label: His-PIMT
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..82
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             89..133
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             149..163
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 aatttttgtt taactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtctggtg ccacgcggtt ctggtatgaa agaaaccgct gctgctaaat tcgaacgcca
      121 gcacatggac agcccagatc tgggtaccga cgacgacgac aaggccatgg cctggaaatc
      181 cggcggcgcc agccactcgg agctaatcca caatctccgc aaaaatggaa tcatcaagac
      241 agataaagta tttgaagtga tgctggctac agaccgctcc cactatgcaa aatgtaaccc
      301 atacatggat tctccacaat caataggttt ccaagcaaca atcagtgctc cacacatgca
      361 tgcatatgcg ctagaacttc tatttgatca gttgcatgaa ggagctaaag ctcttgatgt
      421 aggatctgga agtggaatcc ttactgcatg ttttgcacgt atggttggat gtactggaaa
      481 agtcatagga attgatcaca ttaaagagct agtagatgac tcagtaaata atgtcaggaa
      541 ggacgatcca acacttctgt cttcagggag
//
