LOCUS       sequence-42859-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V678 pDNR MCS SA ES - Donor
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        118..563
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 gatccataac ttcgtatagc atacattata cgaagttatg cggccggccg cgacgtccac
      121 atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt tctgaattgt
      181 gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca gagaatgaaa
      241 agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt ttatgatttt
      301 ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct aatagaatga
      361 ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc taaaatgtgt
      421 aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt tttttattgt
      481 gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt
      541 acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat
      601 taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga
      661 accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc
      721 aaatccctga acagcaaaaa aatgaaaaat ataaagttcc tgagttcgat tcgtccacaa
      781 ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaaca
      841 ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgc
//
