LOCUS       sequence-429398-       10243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral overexpression of human MTHFD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Flickinger KM, Wilson KM, Rossiter NJ, Hunger AL, Vishwasrao PV, Lee
            TD, Mellado Fritz CA, Richards RM, Hall MD, Cantor JR
  TITLE     Conditional lethality profiling reveals anticancer mechanisms of 
            action and drug-nutrient interactions.
  JOURNAL   Sci Adv. 2024 Oct 4;10(40):eadq3591. doi: 10.1126/sciadv.adq3591. 
            Epub 2024 Oct 4.
  PUBMED    39365851
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-10-4"; volume: "10(40):eadq3591. doi"; pages: " 
            10.1126/sciadv.adq3591. Epub 2024 Oct 4"
COMMENT     Sequence Label: pLJC2-MTHFD1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6281..6346
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6394..6511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6560..7070
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6989..7007
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7092..7691
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7092..7111)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7588..7608
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7819..8052
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8124..8245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8161..8180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8215..8234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8285..8420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8440..8459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8441..8459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8469..8486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8469..8485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8478..8500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8627..9082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8714..8733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8924..8945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9108..9212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9213..9281,9282..10073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9431..9450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcagcagcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc gctaccggtt taattaaaaa ggccatggcg ccagcagaaa tcctgaacgg
     3481 gaaggagatc tccgcgcaaa taagggcgag actgaaaaat caagtcactc agttgaagga
     3541 gcaagtacct ggtttcacac cacgcctggc aatattacag gttggcaaca gagatgattc
     3601 caatctttat ataaatgtga agctgaaggc tgctgaagag attgggatca aagccactca
     3661 cattaagtta ccaagaacaa ccacagaatc tgaggtgatg aagtacatta catctttgaa
     3721 tgaagactct actgtacatg ggttcttagt gcagctacct ttagattcag agaattccat
     3781 taacactgaa gaagtgatca atgctattgc acccgagaag gatgtggatg gattgactag
     3841 catcaatgct gggaaacttg ctagaggtga cctcaatgac tgtttcattc cttgtacgcc
     3901 taagggatgc ttggaactca tcaaagagac aggggtgccg attgccggaa ggcatgctgt
     3961 ggtggttggg cgcagtaaaa tagttggggc cccgatgcat gacttgcttc tgtggaacaa
     4021 tgccacagtg accacctgcc actccaagac tgcccatctg gatgaggagg taaataaagg
     4081 tgacatcctg gtggttgcaa ctggtcagcc tgaaatggtt aaaggggagt ggatcaaacc
     4141 tggggcaata gtcatcgact gtggaatcaa ttatgtccca gatgataaaa aaccaaatgg
     4201 gagaaaagtt gtgggtgatg tggcatacga cgaggccaaa gagagggcga gcttcatcac
     4261 tcctgttcct ggcggcgtag ggcccatgac agttgcaatg ctcatgcaga gcacagtaga
     4321 gagtgccaag cgtttcctgg agaaatttaa gccaggaaag tggatgattc agtataacaa
     4381 ccttaacctc aagacacctg ttccaagtga cattgatata tcacgatctt gtaaaccgaa
     4441 gcccattggt aagctggctc gagaaattgg tctgctgtct gaagaggtag aattatatgg
     4501 tgaaacaaag gccaaagttc tgctgtcagc actagaacgc ctgaagcacc ggcctgatgg
     4561 gaaatacgtg gtggtgactg gaataactcc aacacccctg ggagaaggga aaagcacaac
     4621 tacaatcggg ctagtgcaag cccttggtgc ccatctctac cagaatgtct ttgcgtgtgt
     4681 gcgacagcct tctcagggcc ccacctttgg aataaaaggt ggcgctgcag gaggcggcta
     4741 ctcccaggtc attcctatgg aagagtttaa tctccacctc acaggtgaca tccatgccat
     4801 cactgcagct aataacctcg ttgctgcggc cattgatgct cggatatttc atgaactgac
     4861 ccagacagac aaggctctct ttaatcgttt ggtgccatca gtaaatggag tgagaaggtt
     4921 ctctgacatc caaatccgaa ggttaaagag actaggcatt gaaaagactg accctaccac
     4981 actgacagat gaagagataa acagatttgc aagattggac attgatccag aaaccataac
     5041 ttggcaaaga gtgttggata ccaatgatag attcctgagg aagatcacga ttggacaggc
     5101 tccaacggag aagggtcaca cacggacggc ccagtttgat atctctgtgg ccagtgaaat
     5161 tatggctgtc ctggctctca ccacttctct agaagacatg agagagagac tgggcaaaat
     5221 ggtggtggca tccagtaaga aaggagagcc cgtcagtgcc gaagatctgg gggtgagtgg
     5281 tgcactgaca gtgcttatga aggacgcaat caagcccaat ctcatgcaga cactggaggg
     5341 cactccagtg tttgtccatg ctggcccgtt tgccaacatc gcacatggca attcctccat
     5401 cattgcagac cggatcgcac tcaagcttgt tggcccagaa gggtttgtag tgacggaagc
     5461 aggatttgga gcagacattg gaatggaaaa gttttttaac atcaaatgcc ggtattccgg
     5521 cctctgcccc cacgtggtgg tgcttgttgc cactgtcagg gctctcaaga tgcacggggg
     5581 cggccccacg gtcactgctg gactgcctct tcccaaggct tacatacagg agaacctgga
     5641 gctggttgaa aaaggcttca gtaacttgaa gaaacaaatt gaaaatgcca gaatgtttgg
     5701 aattccagta gtagtggccg tgaatgcatt caagacggat acagagtctg agctggacct
     5761 catcagccgc ctttccagag aacatggggc ttttgatgcc gtgaagtgca ctcactgggc
     5821 agaagggggc aagggtgcct tagccctggc tcaggccgtc cagagagcag cacaagcacc
     5881 cagcagcttc cagctccttt atgacctcaa gctcccagtt gaggataaaa tcaggatcat
     5941 tgcacagaag atctatggag cagatgacat tgaattactt cccgaagctc aacacaaagc
     6001 tgaagtctac acgaagcagg gctttgggaa tctccccatc tgcatggcta aaacacactt
     6061 gtctttgtct cacaacccag agcaaaaagg tgtccctaca ggcttcattc tgcccattcg
     6121 cgacatccgc gccagcgttg gggctggttt tctgtacccc ttagtaggaa cgatgagcac
     6181 aatgcctgga ctccccaccc ggccctgttt ttatgatatt gatttggacc ctgaaacaga
     6241 acaggtgaat ggattattcg gaggcagcgc ggccgcaggc gactacaaag accatgacgg
     6301 tgattataaa gatcatgaca ttgattacaa ggatgacgat gacaagtaat aagaattctc
     6361 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     6421 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     6481 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     6541 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     6601 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     6661 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     6721 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     6781 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     6841 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     6901 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     6961 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     7021 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
     7081 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     7141 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7201 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7261 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7321 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7381 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7441 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7501 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7561 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7621 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7681 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     7741 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7801 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7861 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7921 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7981 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8041 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8101 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8221 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8281 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8341 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8401 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     8461 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8521 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8581 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8761 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     9001 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9061 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    10021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    10081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    10141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    10201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
