LOCUS       sequence-429983-       10902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian 
            expression and lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Kim JW, Yong AJH, Aisenberg EE, Lobel JH, Wang W, Dawson TM, Dawson 
            VL, Gao R, Jan YN, Bateup HS, Ingolia NT
  TITLE     Molecular recording of calcium signals via calcium-dependent 
            proximity labeling.
  JOURNAL   Nat Chem Biol. 2024 Jul;20(7):894-905. doi: 
            10.1038/s41589-024-01603-7. Epub 2024 Apr 24.
  PUBMED    38658655
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01603-7"; journalName: "Nat Chem Biol"; date: 
            "2024-07"; volume: "20"; issue: "7"; pages: "894-905"
COMMENT     Sequence Label: pNTI934
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(17..605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(97..116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(776..1567,1568..1636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1637..1741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1816..1835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2007..2386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2388..2586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2541..2561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2604..2784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2831..2956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3449..3682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3867..3911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4209..4326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4391..5602
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5439..5458
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5682..5738
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             join(5739..5747,5748..5762)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             7197..7238
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7363..7951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7416..7436)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7834..7845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7954..7970)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7955..7971)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8476..8656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8682..8699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8688..8912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8958..9386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9045..9064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9255..9276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9395..9415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9400..9729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9580..9715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9642..9661
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9777..9824
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9843..10217
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10347..10468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10384..10403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10438..10457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10517..10533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10517..10533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10530..10552)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10541..10557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10565..10571,10572..10589,10590..10595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10610..10631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10748..10765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
       61 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1741 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1861 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1921 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1981 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     2041 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2101 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2161 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2221 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2281 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2341 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2401 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2461 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2521 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2581 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2761 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2821 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2881 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2941 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3001 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3061 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3121 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3181 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3241 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3301 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3361 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3421 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3481 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3541 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3601 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3661 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3721 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3781 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3841 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3901 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3961 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     4021 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     4081 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4141 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     4201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4321 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     4381 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4441 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4501 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4561 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     4621 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     4681 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4741 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     4801 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     4861 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     4921 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     4981 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5041 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5101 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     5161 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     5221 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5281 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5341 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5401 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     5461 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     5521 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     5581 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatga
     5641 acgacatctt cgaggctcag cgtatcgaat ggcactcatc acgtcgtaag tggaataaga
     5701 caggtcacgc agtcagagct ataggtcggc tgagctcaga tctgtacgac gatgacgata
     5761 agctcgaggg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     5821 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     5881 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     5941 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     6001 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     6061 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     6121 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     6181 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     6241 ctctggccgc tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     6301 gcctggctcc atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga
     6361 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     6421 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     6481 ggtctgccga aaagctgcag ggcggtaccg gagggagcaa agacaatact gtgcctctga
     6541 agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg ggagaaaccc
     6601 tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac tggggcgtgg
     6661 acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg ctgctgaacg
     6721 ctaaacagat tctgggacag ctggacggcc tgccggacca actgactgaa gagcagatcg
     6781 cagaatttaa agaggctttc tccctatttg acaaggacgg ggatgggaca ataacaacca
     6841 aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca gagctgcagg
     6901 acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct gagttcctga
     6961 caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga gaagcgttcg
     7021 gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc cacgtgatga
     7081 caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg gaagcagaca
     7141 tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca gcgaagggca
     7201 agcccatccc caaccccctg ctgggcctgg acagcaccgg cagcggcagc aaaatgagta
     7261 aggatggaaa gaaaaagaaa aagaaaagca aaacaaagtg tgtaataatg tgaggatcca
     7321 ccggtgctag cagttaacga attcgatatc aagcttatcg ataatcaacc tctggattac
     7381 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7441 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7501 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7561 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7621 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7741 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7801 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7861 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7921 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     7981 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     8041 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     8101 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8161 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     8221 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     8281 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     8341 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     8401 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     8461 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8641 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     8701 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8761 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     8821 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     8881 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     8941 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9001 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9061 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9121 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9181 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9241 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9301 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9361 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     9421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9481 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9541 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9601 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9661 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9721 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     9781 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     9841 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     9901 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     9961 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
    10021 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
    10081 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
    10141 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
    10201 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    10261 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10321 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    10381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10441 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    10501 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10561 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10621 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10681 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa gg
//
