LOCUS       sequence-429986-        4197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector control plasmid containing a stop codon prior to the Fc
            encoding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: pFuse-STOP
FEATURES             Location/Qualifiers
     source          1..4197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     polyA_signal    1362..1483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1373..1392)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1427..1446
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    1587..1981
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1995..2369)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        2962..3265
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3345..3933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3425..3444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4041..4089
                     /note="synthetic polyadenylation signal"
     misc_feature    4100..4191
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4140..4159
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgtaaatatc ggccatggtt agatctgaca
      661 aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc
      721 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
      781 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
      841 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
      901 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
      961 aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc
     1021 agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg accaagaacc
     1081 aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg
     1141 agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg
     1201 gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg
     1261 tcttctcatg ctccgtgatg cacgaggctc tgcacaacca ctacacgcag aagagcctct
     1321 ccctgtctcc gggtaaatga gtgctagctg gccagacatg ataagataca ttgatgagtt
     1381 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     1441 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     1501 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     1561 ctacaaatgt ggtatggaat taattctaaa atacagcata gcaaaacttt aacctccaaa
     1621 tcaagcctct acttgaatcc ttttctgagg gatgaataag gcataggcat caggggctgt
     1681 tgccaatgtg cattagctgt ttgcagcctc accttctttc atggagttta agatatagtg
     1741 tattttccca aggtttgaac tagctcttca tttctttatg ttttaaatgc actgacctcc
     1801 cacattccct ttttagtaaa atattcagaa ataatttaaa tacatcattg caatgaaaat
     1861 aaatgttttt tattaggcag aatccagatg ctcaaggccc ttcataatat cccccagttt
     1921 agtagttgga cttagggaac aaaggaacct ttaatagaaa ttggacagca agaaagcgag
     1981 cttctagctt atcctcagtc ctgctcctct gccacaaagt gcacgcagtt gccggccggg
     2041 tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg
     2101 gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg
     2161 ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg
     2221 atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg
     2281 gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc
     2341 accggaacgg cactggtcaa cttggccatg atggctcctc ctgtcaggag aggaaagaga
     2401 agaaggttag tacaattgct atagtgagtt gtattatact atgcagatat actatgccaa
     2461 tgattaattg tcaaactagg gctgcagggt tcatagtgcc acttttcctg cactgcccca
     2521 tctcctgccc accctttccc aggcatagac agtcagtgac ttaccaaact cacaggaggg
     2581 agaaggcaga agcttgagac agacccgcgg gaccgccgaa ctgcgagggg acgtggctag
     2641 ggcggcttct tttatggtgc gccggccctc ggaggcaggg cgctcgggga ggcctagcgg
     2701 ccaatctgcg gtggcaggag gcggggccga aggccgtgcc tgaccaatcc ggagcacata
     2761 ggagtctcag ccccccgccc caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt
     2821 tgtgaaatgg gggcttgggg gggttggggc cctgactagt caaaacaaac tcccattgac
     2881 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     2941 gtactgccaa aaccgcatca tcatggtaat agcgatgact aatacgtaga tgtactgcca
     3001 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     3061 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     3121 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     3181 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     3241 gccatttacc gtaagttatg taacgcctgc aggttaatta agaacatgtg agcaaaaggc
     3301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatggc tagttaatta
     4021 acatttaaat cagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     4081 ttttgtgtga atcgtaacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     4141 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgaa
//
