LOCUS       sequence-430243-        9801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site
            for Cre excision.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP
FEATURES             Location/Qualifiers
     source          1..9801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3406..3415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3415..3480
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3484..3508
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3505..3514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5027..5051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5062..5460
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             5475..6176
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6195..6783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6248..6268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6666..6677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    6919..6952
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6979..7159
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7206..7224)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7292..7396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7397..7465,7466..8257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7615..7634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8428..9016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9170..9187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9427..9448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9463..9468,9469..9486,9487..9493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9501..9517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9506..9528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9525..9541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9525..9541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9591..9610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catggactac
     3421 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3481 cttacaagtt tgtacaaaaa agttgccacc atggatcccg ggcagcagcc gccgcctcaa
     3541 ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg
     3601 tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc
     3661 gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc gctcttcaac
     3721 gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag
     3781 ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccagtact
     3841 gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcattc ctctccagct
     3901 tctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt agtctctggc
     3961 ccagcagcta cacccacagc tcagcatctt cgacagtctt cttttgagat acctgatgat
     4021 gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag atacttctta
     4081 aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct gtcccagatg
     4141 aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc ggcttcaggt
     4201 cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta ctatataaac
     4261 cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt tgccatgaac
     4321 cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc ccagagccca
     4381 cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg actgcagcaa
     4441 ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg gcaggcaatg
     4501 cggaatatca atcccagcac agcaaattct ccaaaatgtc aggagttagc cctgcgtagc
     4561 cagttaccaa cactggagca ggatggtggg actcaaaatc cagtgtcttc tcccgggatg
     4621 tctcaggaat tgagaacaat gacgaccaat agctcagatc ctttccttaa cagtggcacc
     4681 tatcactctc gagatgagag tacagacagt ggactaagca tgagcagcta cagtgtccct
     4741 cgaaccccag atgacttcct gaacagtgtg gatgagatgg atacaggtga tactatcaac
     4801 caaagcaccc tgccctcaca gcagaaccgt ttcccagact accttgaagc cattcctggg
     4861 acaaatgtgg accttggaac actggaagga gatggaatga acatagaagg agaggagctg
     4921 atgccaagtc tgcaggaagc tttgagttct gacatcctta atgacatgga gtctgttttg
     4981 gctgccacca agctagataa agaaagcttt cttacatggt tataataccc aactttcttg
     5041 tacaaagtgg tgtgaagatc cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     5101 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     5161 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     5221 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     5281 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     5341 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     5401 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     5461 ccgcccatat ggccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     5521 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     5581 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     5641 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     5701 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     5761 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5821 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5881 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5941 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     6001 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     6061 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     6121 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaaagga
     6181 tatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6601 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6781 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6841 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tcacctgcag
     6901 gacaggcgcg caggtaccat aacttcgtat aatgtatgct atacgaagtt atggcgcgcc
     6961 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7141 gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg ctagatcatt cttgaagacg
     7201 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7261 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7621 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9181 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9241 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9361 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9421 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9481 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9541 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9601 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9661 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     9721 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9781 atgtactaca aacttagtag t
//
