LOCUS       sequence-430270-        7338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRB1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..7338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    416..537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(427..446)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     481..500
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    641..1035
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1049..1423)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        2016..2319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2399..2987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2479..2498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3095..3143
                     /note="synthetic polyadenylation signal"
     misc_feature    3154..3245
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3194..3213
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3282..3493
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3506..3774
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     3824..3883
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             3884..3910
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5018..5038
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7016..7039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
       61 caaagccaaa gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga
      121 gatgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
      181 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
      241 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
      301 gcagcagggg aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac
      361 gcagaagagc ctctccctgt ctccgggtaa atgagtgcta gctggccaga catgataaga
      421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
      541 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
      601 agcaagtaaa acctctacaa atgtggtatg gaattaattc taaaatacag catagcaaaa
      661 ctttaacctc caaatcaagc ctctacttga atccttttct gagggatgaa taaggcatag
      721 gcatcagggg ctgttgccaa tgtgcattag ctgtttgcag cctcaccttc tttcatggag
      781 tttaagatat agtgtatttt cccaaggttt gaactagctc ttcatttctt tatgttttaa
      841 atgcactgac ctcccacatt ccctttttag taaaatattc agaaataatt taaatacatc
      901 attgcaatga aaataaatgt tttttattag gcagaatcca gatgctcaag gcccttcata
      961 atatccccca gtttagtagt tggacttagg gaacaaagga acctttaata gaaattggac
     1021 agcaagaaag cgagcttcta gcttatcctc agtcctgctc ctctgccaca aagtgcacgc
     1081 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     1141 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     1201 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     1261 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     1321 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     1381 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catgatggct cctcctgtca
     1441 ggagaggaaa gagaagaagg ttagtacaat tgctatagtg agttgtatta tactatgcag
     1501 atatactatg ccaatgatta attgtcaaac tagggctgca gggttcatag tgccactttt
     1561 cctgcactgc cccatctcct gcccaccctt tcccaggcat agacagtcag tgacttacca
     1621 aactcacagg agggagaagg cagaagcttg agacagaccc gcgggaccgc cgaactgcga
     1681 ggggacgtgg ctagggcggc ttcttttatg gtgcgccggc cctcggaggc agggcgctcg
     1741 gggaggccta gcggccaatc tgcggtggca ggaggcgggg ccgaaggccg tgcctgacca
     1801 atccggagca cataggagtc tcagcccccc gccccaaagc aaggggaagt cacgcgcctg
     1861 tagcgccagc gtgttgtgaa atgggggctt gggggggttg gggccctgac tagtcaaaac
     1921 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
     1981 ccacgcccat tgatgtactg ccaaaaccgc atcatcatgg taatagcgat gactaatacg
     2041 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     2101 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     2161 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     2221 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     2281 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ctgcaggtta attaagaaca
     2341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3061 tggctagtta attaacattt aaatcagcgg ccgcaataaa atatctttat tttcattaca
     3121 tctgtgtgtt ggttttttgt gtgaatcgta actaacatac gctctccatc aaaacaaaac
     3181 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
     3241 tctctatcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3301 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     3361 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3421 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3481 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     3541 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     3601 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     3661 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     3721 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     3781 gtgaccggcg cctacctgag atcaccggcg aaggagggcc accatgtaca ggatgcaact
     3841 cctgtcttgc attgcactaa gtcttgcact tgtcacgaat tcgtacccat acgatgttcc
     3901 agattacgct ggatccaaaa ttgaagaagg caaactggtg atttggatta acggcgataa
     3961 aggctataac ggcctggcgg aagtgggcaa aaaatttgaa aaagataccg gcattaaagt
     4021 gaccgtggaa catccggata aactggaaga aaaatttccg caggtggcgg cgaccggcga
     4081 tggcccggat attatttttt gggcgcatga tcgctttggc ggctatgcgc agagcggcct
     4141 gctggcggaa attaccccgg ataaagcgtt tcaggataaa ctgtatccgt ttacctggga
     4201 tgcggtgcgc tataacggca aactgattgc gtatccgatt gcggtggaag cgctgagcct
     4261 gatttataac aaagatctgc tgccgaaccc gccgaaaacc tgggaagaaa ttccggcgct
     4321 ggataaagaa ctgaaagcga aaggcaaaag cgcgctgatg tttaacctgc aggaaccgta
     4381 ttttacctgg ccgctgattg cggcggatgg cggctatgcg tttaaatatg aaaacggcaa
     4441 atatgatatt aaagatgtgg gcgtggataa cgcgggcgcg aaagcgggcc tgacctttct
     4501 ggtggatctg attaaaaaca aacatatgaa cgcggatacc gattatagca ttgcggaagc
     4561 ggcgtttaac aaaggcgaaa ccgcgatgac cattaacggc ccgtgggcgt ggagcaacat
     4621 tgataccagc aaagtgaact atggcgtgac cgtgctgccg acctttaaag gccagccgag
     4681 caaaccgttt gtgggcgtgc tgagcgcggg cattaacgcg gcgagcccga acaaagaact
     4741 ggcgaaagaa tttctggaaa actatctgct gaccgatgaa ggcctggaag cggtgaacaa
     4801 agataaaccg ctgggcgcgg tggcgctgaa aagctatgaa gaagaactgg cgaaagatcc
     4861 gcgcattgcg gcgaccatgg aaaacgcgca gaaaggcgaa attatgccga acattccgca
     4921 gatgagcgcg ttttggtatg cggtgcgcac cgcggtgatt aacgcggcga gcggccgcca
     4981 gaccgtggat gaagcgctga aagatgcgca gaccaacgag aacctttact tccaaagtac
     5041 gttcgcgatc ctggcgcagc tgagcgccga tgctaacatg gagaaggcta cactgcccag
     5101 tgtcacactg atcgtgggtt gtggagtgag ctctctcacg ctcctgatgc tggtcatcat
     5161 ttatgtgagc gtgtggcgct atattcgcag tgaacgcagc gtcattctga tcaacttttg
     5221 ccttagcatt atctcaagca acgcgctgat cctcatcggc caaacgcaga cacgtaacaa
     5281 ggttgtatgt acactggtgg ccgcattcct ccatttcttt ttcctgtctt ccttctgttg
     5341 ggttttgact gaggcctggc aatcctacat ggctgtgacc gggcatctgc gtaaccgttt
     5401 gatccggaag cggtttctgt gcttgggatg gggattgccc gctctggtcg tggctattag
     5461 tgtaggtttc acaaaggcga agggttatag caccatgaac tattgctggc tgtctctcga
     5521 aggaggcttg ctgtatgctt ttgtgggacc cgctgccgct gttgtcctgg tcaacatggt
     5581 gattggtatt ctggtgttta ataagctcgt atctaaggac ggcattaccg acaaaaagct
     5641 gaaggaacgc gctggagcca gcctctggtc cagttgcgtc gtgctcccac ttctcgcact
     5701 cacgtggatg agcgccgtcc tcgccgtgac cgaccggcgc tccgccctgt ttcaaattct
     5761 gttcgcagtg tttgactctc tggagggctt cgttatcgtg atggtccact gtatccttag
     5821 gcgtgaggtg caggatgctg tgaagtgccg tgtcgtagac aggcaggaag agggtaatgg
     5881 cgattccggt ggctctttcc agaacggtca cgcccagctt atgacagact tcgagaagga
     5941 cgtggatctt gcgtgccgct ctgtgctcaa caaggacatc gcagcgtgtc ggactgcaac
     6001 catcaccggc accctgaaaa gacctagcct cccggaagag gaaaagctga agctggctca
     6061 tgcgaaggga ccgcccacaa attttaattc tctgcctgct aatgtcagta agctccacct
     6121 gcacggcagc ccacgctacc ccggtggccc tctccccgat tttcctaacc actcactgac
     6181 cctgaaacgg gataaggctc ccaaaagctc tttcgtggga gacggtgaca tctttaaaaa
     6241 gctcgatagc gagctgagcc gtgcccaaga gaaggccctg gatacatctt acgtgattct
     6301 cccaacagcc acagccacgc tgcgcccaaa gcctaaggaa gagccaaaat actctattca
     6361 catcgatcag atgccccaga ccagacttat ccacctgtcc actgcccctg aggcaagtct
     6421 gcccgcgcgc agcccacctt ctagacagcc gcccagtggt ggcccgcctg aagcaccgcc
     6481 agcgcaaccg ccaccgcctc cacctccgcc tccaccgcct ccgcagcaac cactgccacc
     6541 tccgccaaac cttgaacctg cccctccgtc cctgggtgat cccggagagc cggcggccca
     6601 ccccggtccc tcaaccggac cctccaccaa gaatgagaac gttgccacct tgtccgtttc
     6661 ctcattggaa aggagaaagt ctagatatgc cgaactcgac tttgagaaga ttatgcacac
     6721 ccggaagcgc catcaggaca tgttccagga cctgaaccgg aaactccagc acgccgctga
     6781 aaaagacaaa gaggtgctgg gtccggactc aaagccagag aaacagcaaa ctcccaataa
     6841 gaggccttgg gagtccctgc gcaaggcaca tggaacccca acctgggtta aaaaggagct
     6901 tgagcctctt cagccaagcc cactcgaact gcgctccgtc gagtgggaga gatctggcgc
     6961 gactatccca ctcgtcggcc aggatattat cgacctgcaa acagaggtgg gttctgacta
     7021 caaagacgat gacgacaagt gaagatctga caaaactcac acatgcccac cgtgcccagc
     7081 acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct
     7141 catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc
     7201 tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc
     7261 gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca
     7321 ggactggctg aatggcaa
//
