LOCUS       sequence-430533-        7046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU2 backbone with LacI(YQR) and P(O1)_nanoluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH101
FEATURES             Location/Qualifiers
     source          1..7046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1251..2333
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1270..1289)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(5490..5558,5559..6350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5708..5727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6481..6590
                     /note="incP origin of transfer"
     rep_origin      join(6761..7046,1..103)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggta caaagaaaat tcgacaaact gttatttttc tatctattta tttgaattgt
      181 gagcggataa caattacctt tgtcggcaat tgtgagcgga taacaattaa ataaagatat
      241 tctcgtcaaa caaatataaa taatataaac atggttttta ctctggaaga ttttgttggc
      301 gattggcgtc agaccgcggg ttataatttg gatcaagtcc tggaacaggg tggcgtaagc
      361 tctctgttcc agaacctggg tgtgagcgtg acgccgattc agcgcatcgt tctgtccggc
      421 gagaacggtc tgaaaattga tattcatgtg atcatcccgt acgaaggcct gagcggtgac
      481 caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc cagttgacga tcaccacttc
      541 aaggttatct tgcattacgg tacgctggtg attgatggtg tgaccccgaa tatgattgac
      601 tatttcggcc gtccgtatga aggcattgcc gtttttgacg gtaaaaagat caccgtcacc
      661 ggtaccctgt ggaatggcaa taagattatt gacgagcgtc tgattaaccc ggacggcagc
      721 ctgctgttcc gcgtgaccat caacggtgtc acgggttggc gtctgtgcga gcgcatcctg
      781 gcataatgaa ctgcacttgc tttgataatt aatgataaac aatctaaaag cactctaatc
      841 gttatcggag tgcttttaga ttactaatca aattgcttct actaattgcc tatcttccag
      901 tgatggaaca gcatttgtgc attggctgca acaatcagcc ttgatctgga agaagcaatg
      961 aaagctgctg ttaagtctcc gaatcaggta ttgttcctga caggtgtatt cccatccggt
     1021 aaacgcggat actttgcagt tgatctgact caggaataaa ttataaatta aggtaagaag
     1081 attgtaggat aagctaatga aatagaaaaa ggatgccgtc acacaacttg tcggcattct
     1141 tttttgtttt attagttgaa aatatagtga aaaagttgcc taaatatgta tgttaacaaa
     1201 ttatttgtcg taactttgca ctccaaatct gtttttaaca tatggcacta gtgaaaccag
     1261 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     1321 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     1381 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     1441 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     1501 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     1561 tcgaagcctg taaaacggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     1621 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     1681 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     1741 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     1801 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     1861 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     1921 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     1981 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     2041 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     2101 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     2161 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     2221 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2281 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagctttcc
     2341 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
     2401 tacttgtgcc tgttctattt ccgaaccgac cgcttgtatg aatgcatcaa aattcgtttt
     2461 ctctacgttg gattccttgt tgctcatatt gtgatgataa tttctacaaa tatagtcatt
     2521 ggtaactatc tatgaaactg tttgatactt ttatagttga ttaaacttgt tcatggcatt
     2581 tgccttaata tcatccgcta tgtcaatgta gggtttcata gctttgtagt cgctgtgtcc
     2641 cgtccatttc atgaccacct gtgccgggat tccgagagcc agcgcattgc agatgaatgt
     2701 cctttttcct gcatgggtac tgagcaaagc gtatttgggt gtgacttcat caatacgttc
     2761 atttcccttg tagtaggttt cccgtacagg ctcgttgatt tctgccagtt cacccagctc
     2821 tttcaggtaa tcgttcatct tctggttgct gatgacgggc agagccatgt aattctcgaa
     2881 atggatgtcc ttgtatttgt ccagtatggc tttgctgtat ttgttcagtt caatcgtcag
     2941 gctgtcggca gtcttgactg tggttatttc gatgtggtcg gacttcacat cgcttctttt
     3001 cagattgcga acatccgaat accgcaaact cgtaaagcag cagaacagga aaacatcacg
     3061 cacacgttcc aggtattgct tatccttggg tatctggtag tctttcagct tgttcagttc
     3121 atcccaagtc aggaagatta cttttttcga ggtggttttc agtttcggtt tgaacgtatc
     3181 gtatgcaatg ttctgatgat gtcctttctt gaagctccag cgcaggaacc atttgaggaa
     3241 tcccatttgc ttgccgatgg tgctgtttct catatccttg gtgtcacgca ggaagttgac
     3301 gtattcgttc aatccaaact cgttgaaata gttgaacgtt gcatcctcct tgaactcttt
     3361 gaggtggttc ctcactgctg caaatttttc ataggtggat gccgtccagt tattctggtt
     3421 accgcactct tttacaaact catcgaacac ctcccaaaag ctgacagggg cttcttccgg
     3481 ctgttcttca ctggtgtctt tcattctcat gttgaaagct tccttcaact gttgggtcgt
     3541 tggcatgacc tcctgcacct caaattcctt gaaaatattc tggatttcgg catagtattt
     3601 cagcaagtcc gtattgattt cggctgcact ttgctttagc ttgttggtac atccgttctt
     3661 tacccgctgc ttatctacat cccatttggc tacgtcaatc cggtagcccg ttgtaaactc
     3721 gatacgttgg ctggcaaaga tgacacgcat acggatgggt acgttctcta cgattggcac
     3781 accgttcttt ttccggctct ccaatgcaaa aatgatgttg cgcttgatat tcataattgg
     3841 gtgcgtttga aattctacac ccaaatatac acccaattat tgagatagca aaagacattt
     3901 agaaacattt acttttactc tatattgtaa tttacacttg attatcagtc gtttgcagtt
     3961 ttatgatatt ctgtgaaagt ataagttcga gagcctgtct ctccgcaaaa aacgctgaaa
     4021 atcagcagat tgcaaaacaa acaccctgtt ttacacccaa gaatgtaaag tcgggtgttt
     4081 ttgttttatt taagataata caaccactac ataataaaag agtagcgata ttaaaagaat
     4141 ccgatgagaa aagactaata tttatctatc cattcagttt gatttctcag gactttacat
     4201 cgtcctgaaa gtatttgttg gtaccgagga cgcgtaaaca tttacagttg catgtggcct
     4261 attgttttta gccgttaaat attttataac tattaaatag cgatacaaat tgttcgaaac
     4321 taatattgtt tatatcatat attctcgcat gttttaaagc tttattaaat tgattttttg
     4381 taaacagttt ttcgtactct ttgttaaccc atttcattac aaaagtttca tatttttttc
     4441 tctctttaaa tgccattttt gctggctttc tttttaatac aattaatgtg ctatccactt
     4501 taggttttgg atggaaataa tacctaggaa tttttgctaa tatagaaata tctacctctg
     4561 ccattaacag caatgctagt gatctgtttg tatctaataa cattttagca aaaccatatt
     4621 ccactattaa ataacttatt gtggctgaac tttcaaaaac aatttttcga attatatttg
     4681 tgcttatgtt gtaaggtatg ctgccaaata ttttatatgg attgtggcta ggaaatgtaa
     4741 atttcagtat atcatcattt actatttgat agttaggata atttaagagc ttattacgag
     4801 ttacctcaca taatttagaa tcaatttcta tcgccgttac aaaattacat ctctttacca
     4861 atccagcagt aaaatgacct ttccctgcac ctatttcaaa gatgttatct ttttcatcta
     4921 aacttatgca attcattatt ttttctatgt gatattttga agtaataaaa ttttgactat
     4981 cttttatatt tactttgttc attataacct ctccttaatt tattgcatct cttttcgaat
     5041 atttatgttt tttgagaaaa gaacgtactc atggttcatc ccgatatgcg tatcggtctg
     5101 tatatcagca actttctatg tgtttcaact acaatagtca tctattctca tctttctgag
     5161 tccaccccct gcaaagcccc tctttacgac ataaaaattc ggtcggaaaa ggtatgcaaa
     5221 agatgtttct ctctttaaga gaaactcttc gggatgcaaa aatatgaaaa taactccaat
     5281 tcaccaaatt atatagcgac ttttttacaa aatgctaaaa tttgttgatt tccgtcaagc
     5341 aattgttgag caaaaatgtc ttttacgata aaatgatacc tcaatatcaa ctgtttagca
     5401 aaacgatatt tctcttaaag agagaaacac ctttttgttc accaatcccc gacttttaat
     5461 cccgcggcca tgattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5521 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5581 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5641 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5701 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5761 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5821 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5881 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5941 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6001 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6061 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6121 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6181 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6241 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6301 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6361 aagtttactc ataacgcgtc aattcgaggg ggatcaattc cgtgataggt gggctgccct
     6421 tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag
     6481 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
     6541 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
     6601 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     6661 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
     6721 aaaacggaat tgatccggcc acgatgcgtc cggcgtagag gatctgaaga tcagcagttc
     6781 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
     6841 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
     6901 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
     6961 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
     7021 tatataaggc ttttaaagct tttaag
//
