LOCUS       sequence-430545-        5216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU2 backbone with BsmBI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH001
FEATURES             Location/Qualifiers
     source          1..5216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(150..155,156..173,174..180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    188..204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     193..215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     212..228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     212..228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             224..547
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    241..297
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(298..315)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(298..314)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(307..329)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(523..542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             join(3660..3728,3729..4520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3878..3897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4651..4760
                     /note="incP origin of transfer"
     rep_origin      join(4931..5216,1..103)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggcc cttgagacgg catcttccat ctacacttta tgcttccggc tcgtatgttg
      181 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      241 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
      301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      421 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      481 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcataggat ctgatgcatc gtctcaccga tacttgtgcc tgttctattt ccgaaccgac
      601 cgcttgtatg aatgcatcaa aattcgtttt ctctacgttg gattccttgt tgctcatatt
      661 gtgatgataa tttctacaaa tatagtcatt ggtaactatc tatgaaactg tttgatactt
      721 ttatagttga ttaaacttgt tcatggcatt tgccttaata tcatccgcta tgtcaatgta
      781 gggtttcata gctttgtagt cgctgtgtcc cgtccatttc atgaccacct gtgccgggat
      841 tccgagagcc agcgcattgc agatgaatgt cctttttcct gcatgggtac tgagcaaagc
      901 gtatttgggt gtgacttcat caatacgttc atttcccttg tagtaggttt cccgtacagg
      961 ctcgttgatt tctgccagtt cacccagctc tttcaggtaa tcgttcatct tctggttgct
     1021 gatgacgggc agagccatgt aattctcgaa atggatgtcc ttgtatttgt ccagtatggc
     1081 tttgctgtat ttgttcagtt caatcgtcag gctgtcggca gtcttgactg tggttatttc
     1141 gatgtggtcg gacttcacat cgcttctttt cagattgcga acatccgaat accgcaaact
     1201 cgtaaagcag cagaacagga aaacatcacg cacacgttcc aggtattgct tatccttggg
     1261 tatctggtag tctttcagct tgttcagttc atcccaagtc aggaagatta cttttttcga
     1321 ggtggttttc agtttcggtt tgaacgtatc gtatgcaatg ttctgatgat gtcctttctt
     1381 gaagctccag cgcaggaacc atttgaggaa tcccatttgc ttgccgatgg tgctgtttct
     1441 catatccttg gtgtcacgca ggaagttgac gtattcgttc aatccaaact cgttgaaata
     1501 gttgaacgtt gcatcctcct tgaactcttt gaggtggttc ctcactgctg caaatttttc
     1561 ataggtggat gccgtccagt tattctggtt accgcactct tttacaaact catcgaacac
     1621 ctcccaaaag ctgacagggg cttcttccgg ctgttcttca ctggtgtctt tcattctcat
     1681 gttgaaagct tccttcaact gttgggtcgt tggcatgacc tcctgcacct caaattcctt
     1741 gaaaatattc tggatttcgg catagtattt cagcaagtcc gtattgattt cggctgcact
     1801 ttgctttagc ttgttggtac atccgttctt tacccgctgc ttatctacat cccatttggc
     1861 tacgtcaatc cggtagcccg ttgtaaactc gatacgttgg ctggcaaaga tgacacgcat
     1921 acggatgggt acgttctcta cgattggcac accgttcttt ttccggctct ccaatgcaaa
     1981 aatgatgttg cgcttgatat tcataattgg gtgcgtttga aattctacac ccaaatatac
     2041 acccaattat tgagatagca aaagacattt agaaacattt acttttactc tatattgtaa
     2101 tttacacttg attatcagtc gtttgcagtt ttatgatatt ctgtgaaagt ataagttcga
     2161 gagcctgtct ctccgcaaaa aacgctgaaa atcagcagat tgcaaaacaa acaccctgtt
     2221 ttacacccaa gaatgtaaag tcgggtgttt ttgttttatt taagataata caaccactac
     2281 ataataaaag agtagcgata ttaaaagaat ccgatgagaa aagactaata tttatctatc
     2341 cattcagttt gatttctcag gactttacat cgtcctgaaa gtatttgttg gtaccgagga
     2401 cgcgtaaaca tttacagttg catgtggcct attgttttta gccgttaaat attttataac
     2461 tattaaatag cgatacaaat tgttcgaaac taatattgtt tatatcatat attctcgcat
     2521 gttttaaagc tttattaaat tgattttttg taaacagttt ttcgtactct ttgttaaccc
     2581 atttcattac aaaagtttca tatttttttc tctctttaaa tgccattttt gctggctttc
     2641 tttttaatac aattaatgtg ctatccactt taggttttgg atggaaataa tacctaggaa
     2701 tttttgctaa tatagaaata tctacctctg ccattaacag caatgctagt gatctgtttg
     2761 tatctaataa cattttagca aaaccatatt ccactattaa ataacttatt gtggctgaac
     2821 tttcaaaaac aatttttcga attatatttg tgcttatgtt gtaaggtatg ctgccaaata
     2881 ttttatatgg attgtggcta ggaaatgtaa atttcagtat atcatcattt actatttgat
     2941 agttaggata atttaagagc ttattacgag ttacctcaca taatttagaa tcaatttcta
     3001 tcgccgttac aaaattacat ctctttacca atccagcagt aaaatgacct ttccctgcac
     3061 ctatttcaaa gatgttatct ttttcatcta aacttatgca attcattatt ttttctatgt
     3121 gatattttga agtaataaaa ttttgactat cttttatatt tactttgttc attataacct
     3181 ctccttaatt tattgcatct cttttcgaat atttatgttt tttgagaaaa gaacgtactc
     3241 atggttcatc ccgatatgcg tatcggtctg tatatcagca actttctatg tgtttcaact
     3301 acaatagtca tctattctca tctttctgag tccaccccct gcaaagcccc tctttacgac
     3361 ataaaaattc ggtcggaaaa ggtatgcaaa agatgtttct ctctttaaga gaaactcttc
     3421 gggatgcaaa aatatgaaaa taactccaat tcaccaaatt atatagcgac ttttttacaa
     3481 aatgctaaaa tttgttgatt tccgtcaagc aattgttgag caaaaatgtc ttttacgata
     3541 aaatgatacc tcaatatcaa ctgtttagca aaacgatatt tctcttaaag agagaaacac
     3601 ctttttgttc accaatcccc gacttttaat cccgcggcca tgattgaaaa aggaagagta
     3661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3901 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3961 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4021 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4081 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4141 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4201 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4261 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4321 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4381 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4441 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4501 cactgattaa gcattggtaa ctgtcagacc aagtttactc ataacgcgtc aattcgaggg
     4561 ggatcaattc cgtgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc
     4621 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
     4681 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     4741 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     4801 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     4861 taatgacccc gaagcagggt tatgcagcgg aaaacggaat tgatccggcc acgatgcgtc
     4921 cggcgtagag gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca
     4981 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
     5041 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
     5101 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
     5161 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaag
//
